Abstract
In this study, some physiological and biochemical parameters related to oxidative stress and the concentration of heavy metals were analyzed in leaves of pine trees (Pinus sylvestris L.) collected from regions suspected of air pollution. Leaves were collected from plants at five different locations near Erzincan, Turkey including the city center and a control area. All leaves were divided into two main parts after a washing procedure. After all pre-procedures in the laboratory, heavy metal analysis was conducted using inductively coupled plasma-optical emission spectrometer (ICP-OES). Data were analyzed statistically, and significant differences were found for plants collected among the different locations. Additionally, electrolyte leakage and physiological parameters such as lipid peroxidation, H2O2 content, chlorophyll content, and activity of the antioxidant enzyme superoxide dismutase were determined as indicators of plant cell stress. Results indicated significant differences between plants at experimental locations vs. the control area in terms of stress parameters; these differences were associated with heavy metal concentrations in plants.
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Ananieva EA, Alexieva VS, Popova LP (2002) Treatment with salicylic acid decreases the effects of paraquat on photosynthesis. J Plant Physiol 159:685–693
Anonymous (2014) Erzincan İli 2013 Yılı Çevre Durum Raporu. Erzincan page 16
Anonymous (2015) http://www.erzincan.gov.tr/erzincan/cografi-yapisi/
Aksoy A, Leblebici Z, Prasad MNV (2015) Metal-accumulating plants from serpentine habitats of Kızıldaag, Konya Province, Turkey. Aust J Bot 63(4):372–378
Bramryd T (2013) Long-term effects of sewage sludge application on the heavy metal concentrations in acid pine (Pinus sylvestris L.) forests in a climatic gradient in Sweden. Forest Eco Manage 289:434–444
Baycu G, Tolunay D, Ozden H, Gunebakan S (2006) Ecophysiological and seasonal variations in Cd, Pb, Zn, and Ni concentrations in the leaves of urban deciduous trees in Istanbul. Environ Poll 143:545–554
Darrall NM (1989) The effect of air pollutants on physiological processses in plants. Plant Cell Environ 12(1):1–30
Demirezen D, Aksoy A (2006) Heavy metal levels in vegetables in Turkey is within safe limits for Cu, Zn, Ni and exceeded for Cd and Pb. J Food Qua 29:252–265
De Vos CHR, Schat H, Waal MAM, Vooijs R, Ernst WHO (1991) Incresed resistance to copper induced damage of the root cell plasmalemma in copper tolerant Siline cucubalus. Physiol Plant 82:523–528
Dhindsa RA, Pand PD, Thorpe TA (1981) Leaf senescence correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 126:93–101
Esim N, Atici O, Mutlu S (2014) Effects of exogenous nitric oxide in wheat seedlings 22 under chilling stress. Toxicol Ind Health 30:268–274
Erdal S (2012) Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress. Plant Physiol Bioche 57:1–7
Griffith M, Ala P, Yang DSC, Hon WC, Moffatt BA (1992) Antifreeze protein produced endogenously in winter rye leaves. Plant Physiol 100:593–596
He YL, Liu YL, Cao WX, Huai MF, Xu BG, Huang BG (2005) Effects of salicylic acid on heat tolerance associated with antioxidant metabolism in Kentucky bluegrass. Crop Sci 45:988–995
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Kabata-Pendias A, Pendias H (1986) Trace Elements in Soils and Plants. CRC Press Inc, Boca Raton, Florida
Karakoyun G, Osma E (2015) Accumulation of heavy metals in Scots pine (Pinus sylvestris L. var. hamata Steven.) depending on air pollution in Erzincan. GUSTIJ 5(2):67–77
Lee EH (2000) Early detection mechanisms of tolerance and amelioration of ozone stress in crop plants. Agrowall, S.B. Environmental Pollution and Plant Responses. CRC Press LLC. 45–81.
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: measurementand characterization by UV-VIS spectroscopy. In: Wrolstad RE (ed) Current Protocols in Food Analytical Chemistry. John Wiley and Sons, New York, pp F4.3.1–F4.3.8
Mingorance MD, Oliva SR (2006) Heavy metals content in N. oleander leaves as urban pollution assessment. Environ Mon Assess 119:57–68
Mutlu S, Atici O, Nalbantoglu B (2009) Effects of salicylic acid and salinity on apoplastic antioxidant enzymes in two wheat cultivars differing in salt tolerance. Bio Plantarum 53:334–338
Mutlu S, Atici O, Esim N, Mete E (2011) Essential oils of catmint (Nepeta meyeri Benth.) induce oxidative stress in early seedlings of various weed species. Acta Physiol Plantarum 33:943–951
Nanos GD, Ilias F (2007) Effects of inert dust on olive (Olea europaea L.) leaf physiological parameters. Environ Sci Pollut Res Int 14(3):212–4
Onder S, Dursun S (2006) Airborne heavy metal pollution of Cedrus libani (A. Rich.) in the city centre of Konya (Turkey). Atmos Environ 40:1122–1133
Osma E, İlhan V, Yalçın İE (2014) Heavy metals accumulation causes toxicological effects in aquatic Typha Domingensis Pers. Braz J Bot 37(4):461–467
Papa S, Bartoli G, Nacca F, D’Abrosca B, Cembrola E, Pellegrino A, Fiorentino A, Fuggi A, Fioretto A (2012) Trace metals, peroxidase activity, PAHs contents and ecophysiological changes in Quercus ilex leaves in the urban area of Caserta (Italy). J Environ Manage 113:501–509
Pietrzykowski M, Socha J, van Doorn NS (2014) Linking heavy metal bioavailability (Cd, Cu, Zn and Pb) in Scots pine needles to soil properties in reclaimed mine areas. Sci Tot Environ 470–471:501–510
Pulcinelli P, Anselmi N, Bragaloni M (1998) Peroxidase: usablemarkers of airpollution in treesfrom urban environments. Chemosphere 36(4-5):889–894
Raajasubramanian D, Sundaramoorthy P, Baskaran L (2011) Cement dust pollution on growth and yield attributes of groundnut (Arachis hypogaea L.). Inter J Mult Res App 1(1):31–36
Reimann C, Koller F, Kashulina G, Niskavaara H, Englmaier E (2001) Influence of extreme pollution on the inorganic chemical composition of some plants. Environ Poll 115:239–252
Ross SM (1994) Sources and forms of potentially toxic metals in soil–plant systems. In: Ross SM (ed) Toxic Metals in Soil–Plant Systems. Wiley, London, pp 3–26
Shanker AK, Cervantes C, Loza-Tavera H, Avudainayagam S (2005) Chromium toxicity in plants. Environ Int 31:739–753
Ots K, Mandre M (2012) Monitoring of heavy metals uptake and allocation in Pinus sylvestris organs in alkalised soil. Environ Monit Assess 184:4105–4117
Taylor GE, Pitelka LF (1992) Genetic diversity of plant populations and the role of air pollution. In: Barker JR, Tingey DT (eds) Air pollution effects on biodiversty. Van Nostrand Reinhold, New York, p 111
Thounaojam TC, Pandaa P, Mazumdar P, Kumar D, Sharma GD, Sahoo L, Panda SK (2012) Excess copper induced oxidative stress and response of antioxidants in rice. Plant Physiol Bioche 53:33–39
Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growin grice plants. Plant Sci 164:645–655
Wahsha M, Bini C, Fontana S, Wahsha A, Ziliolia D (2012) Toxicity assessment of contaminated soils from a miningarea in Northeast Italy by using lipid peroxidation assay. J Geo Expl 113:112–117
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This study was supported by the Erzincan University BAP (FEN-C-YLP-220114-0061).
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Osma, E., Elveren, M. & Karakoyun, G. Heavy metal accumulation affects growth of Scots pine by causing oxidative damage. Air Qual Atmos Health 10, 85–92 (2017). https://doi.org/10.1007/s11869-016-0410-7
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DOI: https://doi.org/10.1007/s11869-016-0410-7