Abstract
Purpose
The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC).
Materials and methods
In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinoma patients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p.
Results
We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCC patients’ plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro.
Conclusion
In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCC patient plasma compared to that in normal individuals.
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Acknowledgments
This work was supported by the Natural Science Foundation of Hubei (No. 2011cdb513).
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The authors have no conflict of interest.
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W.-B. Zhang and Z.-Q. Pan contributed equally to this work.
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Zhang, W.B., Pan, Z.Q., Yang, Q.S. et al. Tumor suppressive miR-509-5p contributes to cell migration, proliferation and antiapoptosis in renal cell carcinoma. Ir J Med Sci 182, 621–627 (2013). https://doi.org/10.1007/s11845-013-0941-y
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DOI: https://doi.org/10.1007/s11845-013-0941-y