Abstract
Background
Glycodelin plays an important role in normal and abnormal reproductive growth. The in vitro properties of glycodelin have been studied in K562 cells. The role of glycodelin in vivo in the peripheral circulation has not been determined.
Aim
To determine if circulating leukocytes in normal subjects express the glycodelin gene, with a view to understanding its contribution to various disease processes.
Method
RT-PCR analysis of mononuclear cells isolated from the blood of healthy volunteers was used to show glycodelin mRNA expression. Immunocytostaining was performed using anti-glycodelin antibody.
Results
PCR analysis illustrated the expression of the glycodelin gene in peripheral mononuclear cells. Glycodelin protein in these cells was confirmed with immunostaining.
Conclusion
The presence of glycodelin in peripheral white blood cells was demonstrated. The ability of glycodelin to stimulate angiogenesis and act as an immunosuppressive agent could be an indicator of the role of peripheral leukocytes in various disease states.
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References
Bohn H, Kraus W, Winckler W (1982) New soluble placental tissue proteins: their isolation, characterization, localization and quantification. Placenta 4(Suppl):67–81
Logdberg L, Wester L (2000) Immunocalins: a lipocalin subfamily that modulates immune and inflammatory responses. Biochem Biophys Acta 1482(1–2):284–297
Yaniv E, Borovsky Z, Mishan-Eisenberg G, Rachmilewitz J (2003) Placental protein 14 regulates selective B cell responses. Cell Immunol 222:156–163
Okamoto N, Uchida A, Takakura K et al (1991) Suppression by human placental protein 14 of natural killer cell activity. Am J Reprod Immunol 26(4):137–142
Oehninger S, Coddington CC, Hodgen GD, Seppala M (1995) Factors affecting fertilization: endometrial placental protein 14 reduces the capacity of human spermatozoa to bind to the human zona pellucida. Fertil Steril 63:377–383
Morrow DM, Xiong N, Getty RR et al (1994) Hematopoietic placental protein 14, an immunosuppressive factor in cells of the megakaryocytic lineage. Am J Pathol 145:1485–1495
Bolton AE, Pockley AG, Clough KJ et al (1987) Identification of placental protein14 as an immunosuppressive factor in human reproduction. Lancet 1:593–595
Pockley AG, Barrett CL, Bolton AE (1989) Placental protein 14(PP14) content and immunosuppressive activity of human cervical mucus. Symp Soc Exp Biol 43:317–323
Song M, Ramaswamy S, Ramachandran S et al (2001) Angiogenic role for glycodelin in tumorigenesis. PNAS 98(16):9265–9270
Horowitz IR, Cho CH, Song MQ et al (2001) Plasma glycodelin levels in gynecological malignancies. Int J Gynecol Cancer 11:173–179
Podder AS, Kim JG, Gill KP et al (1998) Generation and characterization of a polyclonal antipeptide antibody to human glycodelin. Fertil Steril 69:543–548
Ramachandran S, Ramaswamy S, Cho CH, Parthasarathy S (1998) Lysophosphatidic acid induces glycodelin gene expression in cancer cells. Cancer Lett 177(2):197–202
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Lang, E.E., Venkatraman, G. Glycodelin gene expression in human peripheral white blood cells. Ir J Med Sci 176, 101–104 (2007). https://doi.org/10.1007/s11845-007-0019-9
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DOI: https://doi.org/10.1007/s11845-007-0019-9