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EPG monitoring of the probing behaviour of the common brown leafhopper Orosius orientalis on artificial diet and selected host plants

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Abstract

The common brown leafhopper Orosius orientalis (Hemiptera: Cicadellidae) is a polyphagous vector of a range of economically important pathogens, including phytoplasmas and viruses, which infect a diverse range of crops. Studies on the plant penetration behaviour by O. orientalis were conducted using the electrical penetration graph (EPG) technique to assist in the characterisation of pathogen acquisition and transmission. EPG waveforms representing different probing activities were acquired from adult O. orientalis probing in planta, using two host species, tobacco Nicotiana tabacum and bean Phaseolus vulgaris, and in vitro using a simple sucrose-based artificial diet. Five waveforms (O1–O5) were evident when O. orientalis fed on bean, whereas only four waveforms (O1–O4) and three waveforms (O1–O3) were observed when the leafhopper fed on tobacco and on the artificial diet, respectively. Both the mean duration of each waveform and waveform type differed markedly depending on the food substrate. Waveform O4 was not observed on the artificial diet and occurred relatively rarely on tobacco plants when compared with bean plants. Waveform O5 was only observed with leafhoppers probing on beans. The attributes of the waveforms and comparative analyses with previously published Hemipteran data are presented and discussed, but further characterisation studies will be needed to confirm our suggestions.

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Acknowledgments

This research was funded by Horticulture Australia Limited and the Tobacco Research and Development Corporation with in-kind support from the Department of Primary Industries (DPI), Victoria, Australia. The assistance of Dr Kim Andrews (DPI Rutherglen), Gary Baxter (DPI Ovens) and Lucy Tran-Nguyen (Charles Darwin University, Australia) is gratefully acknowledged.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Queensland, GPO Box 2434, Brisbane, 4001, Australia

    Piotr Trębicki & Rob M. Harding

  2. Department of Primary Industries (DPI), Biosciences Research Division, RMB 1145, Chiltern Valley Road, Rutherglen, VIC, 3685, Australia

    Piotr Trębicki & Kevin S. Powell

  3. Laboratory of Entomology, Wageningen University, PO Box 8031, 6700 EH, Wageningen, The Netherlands

    W. Fred Tjallingii

  4. DPI, Private Bag 15, Ferntree Gully Delivery Centre, Knoxfield, VIC, 3180, Australia

    Brendan C. Rodoni

Authors
  1. Piotr Trębicki
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  2. W. Fred Tjallingii
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  3. Rob M. Harding
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  4. Brendan C. Rodoni
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  5. Kevin S. Powell
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Correspondence to Kevin S. Powell.

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Handling Editor: Guy Smagghe.

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Trębicki, P., Tjallingii, W.F., Harding, R.M. et al. EPG monitoring of the probing behaviour of the common brown leafhopper Orosius orientalis on artificial diet and selected host plants. Arthropod-Plant Interactions 6, 405–415 (2012). https://doi.org/10.1007/s11829-012-9192-5

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  • DOI: https://doi.org/10.1007/s11829-012-9192-5

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