Abstract
Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitin-inducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.
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Abbreviations
- Bar:
-
Phosphinothricin acetyltransferase
- PPT:
-
Phosphinothricin
- RT-PCR:
-
Reverse transcriptase polymerase chain reaction
- T3 :
-
Transgenic third generation
- ZjCIGR1 :
-
Zoysia japonica chitin-inducible gibberellin-responsive 1 gene
- 35S:
-
CaMV 35S promoter
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Acknowledgements
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A1A03012862), and by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bioindustry Technology Development Project, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA; Grant Number: 315025-3). The grantors had not played any role in writing this report or the decision to submit this article for publication.
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Kim, YJ., Yang, DH., Park, MY. et al. Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass. Plant Biotechnol Rep 14, 21–31 (2020). https://doi.org/10.1007/s11816-019-00570-z
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DOI: https://doi.org/10.1007/s11816-019-00570-z