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Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source

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Abstract

Trichosporon oleaginosus is one of the most promising hosts for microbial lipid production owing to its high-productivity; In an effort to develop an economical production process, we engineered T. oleaginosus towards high-lipid production from volatile fatty acids (VFA) derived from anaerobic fermentation of food waste. First, we established a method for labeling intracellular lipid with lipophilic BODIPY fluorescent dye. Next, a random library was constructed by treatment with a chemical mutagen, and high-lipid producers were screened using fluorescence-activated cell sorting. Subsequently, one clone, N14, was successfully isolated, which exhibited 3-fold higher lipid production (19.4%) in VFA (6 g/L) media than the wild-type strain, and also showed increased lipid production in higher concentrations of VFA (18 or 24 g/L). Based on fatty acid methyl ester (FAME) analysis, N14 contained higher stearic acid (C18:0) and oleic acid (C18:1) content compared with those of the wild-type strain.

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Author notes

  1. The first and second authors have identical collaboration in this paper.

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Kyungsoo Lee & Ki Jun Jeong

  2. Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Korea

    Yong Jae Lee

  3. Nurikkum Square R&D Center 1013-2, Lab to Market, Inc., 396 World Cup buk-ro, Mapo-gu, Seoul, 03925, Korea

    Ho Nam Chang

  4. Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Korea

    Ki Jun Jeong

Authors
  1. Kyungsoo Lee
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  2. Yong Jae Lee
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  3. Ho Nam Chang
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  4. Ki Jun Jeong
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Correspondence to Ki Jun Jeong.

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Lee, K., Lee, Y.J., Chang, H.N. et al. Engineering Trichosporon oleaginosus for enhanced production of lipid from volatile fatty acids as carbon source. Korean J. Chem. Eng. 36, 903–908 (2019). https://doi.org/10.1007/s11814-018-0229-7

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  • DOI: https://doi.org/10.1007/s11814-018-0229-7

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