Abstract
Enzymatic hydrolysis and fermentation methods were evaluated on alkaline peroxide pretreated shea tree sawdust conversion to ethanol. Optimum pretreatment conditions of 120 °C reaction temperature, 30 min reaction time, and 20 mL L−1 of water hydrogen peroxide concentration (1%(v/v)H2O2) solubilized 679 g kg−1 of hemicellulose and 172 g kg−1 of lignin. 617 g kg−1 cellulose was retained in the solid fraction. The maximum yield of reducing sugar with optimized enzyme loadings by two enzyme preparations (cellulase and β-glucosidase) was 165 g kg−1 of dry biomass. The ethanol yield was 7.35 g L−1 after 72 h incubation period under the following conditions: 2% cellulose loading, enzyme concentration was 25 FPU (g cellulose)−1 loading, yeast inoculums was 10% (v/v), 32 oC, and pH 4.8. The pretreatments gave information about the hindrances caused by lignin presence in lignocellulosic materials and that hemicelluloses are better hydrolyzed than lignin, thereby enhancing enzymatic digestibility of the sawdust material.
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H. Monique, A. Faaij, R. van den Broek, G. Berndes, D. Gielen and W. Turkenburg, Biomass Bioenergy, 25, 119 (2003).
Y. Lin and S. Tanaka, Appl. Microbiol. Biotechnol., 69, 627 (2006).
J. R. Mielenz, Curr. Opin. Microbiol., 4, 324 (2001).
O. J. Sánchez and C. A. Cardona, Bioresour. Technol., 99, 5270 (2008).
G. Sallé, J. Boussim, A. Raynal-Roques and F. Brunck, Bois et Foréts des Tropiques, 228, 11 (1991).
W. E. Kaar and M.T. Holtzapple, Biomass Bioenerg., 18, 189 (1999).
J.M. Gould, Biotechnol. Bioeng., 24, 46 (1984).
J.M. Gould, Biotechnol. Bioeng., 27, 225 (1985).
B. C. Saha and M. A. Cotta, Biotechnol. Prog., 22, 449 (2006).
B.C. Saha and M. A. Cotta, Enzyme Microbiol. Technol., 41, 528 (2007).
L. Dawson and R. Boopathy, Bioresour. Technol., 98, 1695 (2006).
A. O. Ayeni, S. Banerjee, J. A. Omoleye, F. K. Hymore, B. S. Giri, S.C. Deskmukh, R. A. Pandey and S. N. Mudliar, Biomass Bioenerg., 48, 130 (2013).
A. O. Ayeni, F. K. Hymore, S. N. Mudliar, S. C. Deskmukh, D. B. Satpute, J. A. Omoleye and R. A. Pandey, Fuel, 106, 187 (2013).
D. C. Montgomery, Design and analysis of experiments, Wiley, New York (2001).
P. Mathews, Design of experiments with MINITAB, Pearson Education Publications, New-Delhi (2005).
S. Kim and M. T. Holtzapple, Bioresour. Technol., 96, 1994 (2005).
V. S. Chang, M. Nagwani, C. Kim and M. T. Holtzapple, Appl. Biochem. Biotechnol., 94, 1 (2001).
C. D. Blasi, G. Signorelli, C.D. Russo and G. Rea, Ind. Eng. Chem. Res., 38, 2216 (1999).
S. Li, S. Xu, S. Liu, C. Yang and Q. Lu, Fuel Process Technol., 85, 1201 (2004).
L. Lin, R. Yan, Y. Liu and W. Jiang, Bioresour. Technol., 101, 8217 (2010).
A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter and D. Templeton, US NREL Report No.: TP-510-42618 Contract No.: DE-AC36-99-G010337 (2008).
M. Falls and M. T. Holtzapple, Appl. Biochem. Biotechnol., 165, 506 (2011).
N. Dowe and J. McMillan J, US NREL Report No.: TP-510-42630 Contract No.: DE-AC36-99-G010337 (2008).
G. L. Miller, Anal. Chem., 31, 426 (1959).
F. Ma, N. Yang, C. Xu, H. Yu, J. Wu and X. Zhang, Bioresour. Technol., 101, 9600 (2010).
C. Bennet, American J. Med. Technol., 37, 217 (1971).
M. Nagwani, M, M.S. thesis, Texas A&M University, College Station, Texas (1992).
N. Silanikove, Bioresour. Technol., 48, 71 (1994).
V. S. Chang and M. T. Holtzapple, Appl. Biochem. Biotechnol., 84- 86, 5 (2000).
R. Sutcliffe and J. N. Saddler, Biotechnol. Bioeng. Symp., 17, 749 (1986).
H. Palonen, A. B. Thomsen, M. Tenkanen, A. S. Schmidt and L. Viikari, Appl. Biochem. Biotechnol., 117, 1 (2004).
D. J. Gregg, A. Boussaid and J. N. Saddler, Bioresour. Technol., 63, 7 (1998).
C. Tengborg, M. Galbe and G. Zacchi, Biotechnol. Prog., 17, 110 (2001).
S. K. Sharma, K. L. Kalra and H. S. Grewal, Biomass Bioenergy, 23, 237 (2002).
R. C. Kuhad, R. Gupta, Y. P. Khasa and A. Singh, Bioresour. Technol., 101, 8348 (2010).
F. Ramon-portugal, H. Pingaud and P. Strehaiano, Biotechnol. Lett., 26, 1671 (2004).
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Ayeni, A.O., Omoleye, J.A., Mudliar, S. et al. Utilization of lignocellulosic waste for ethanol production : Enzymatic digestibility and fermentation of pretreated shea tree sawdust. Korean J. Chem. Eng. 31, 1180–1186 (2014). https://doi.org/10.1007/s11814-014-0026-2
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DOI: https://doi.org/10.1007/s11814-014-0026-2