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Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c

  • Materials (Organic, Inorganic, Electronic, Thin Films)
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Abstract

Silver nanoplates (AgNPs) were used as a colorimetric indicator of pH-induced conformational changes in the protein, cytochorme c (cyt c). Cyt c was covalently bound to the surface of AgNPs, and UV-vis spectroscopy was used to identify changes in the optical properties of the resulting cyt c-AgNP conjugates in colloidal solution over pH, with values ranging from 3.1 to 10.6. Transmission electron microscopy (TEM) results indicated that the morphological changes in cyt c-AgNP conjugates were dependent on pH. Moreover, pH-induced conformational changes in cyt c could be detected by visual inspection of the color changes in cyt c-AgNPs, which occurred as a result of the coupling effect of localized surface plasmon resonance (LSPR) by aggregated AgNPs.

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Authors and Affiliations

  1. Department of Chemical Engineering, Kwangwoon University, Wolgye-dong, Nowon-gu, Seoul, 139-701, Korea

    Junsu Park, Do-Young Yoon & Younghun Kim

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  1. Junsu Park
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  2. Do-Young Yoon
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  3. Younghun Kim
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Correspondence to Younghun Kim.

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Park, J., Yoon, DY. & Kim, Y. Applications of silver nanoplates as colorimetric indicators of pH-induced conformational changes in cytochrome c. Korean J. Chem. Eng. 26, 258–260 (2009). https://doi.org/10.1007/s11814-009-0043-8

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  • DOI: https://doi.org/10.1007/s11814-009-0043-8

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