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Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production

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Abstract

Recombinant E. coli strains with 1–3 copies of lacZ genes on their chromosomes were constructed and their β-galactosidase (β-gal) expressions were examined. Serial dilution cultures were used to analyze the long-term genetic stability of the recombinant lacZ genes of the chromosomal or plasmid expression system. The strain with a 3-copy lacZ on the chromosome has a sustainable β-gal expression through 60 hours. However, the β-gal activity of the plasmid expression system lasted less than 36 hours under a no selection condition. Obviously, the genetic stability of the chromosomal expression system demonstrated in this study is better than that of the plasmid expression system under nonselective condition, such as a medium without antibiotics. The results demonstrated that the strains with a multiple-copy-gene on the chromosome are useful for protein production in industrial repeated fed-batch fermentation.

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Authors and Affiliations

  1. Institute of Biotechnology, Department of Chemical Engineering, National Taipei University of Technology, No.1, Sec. 3, Chung-Hsiao E. Rd, Taipei, 10608, Taiwan, China

    Hsing-Ta Chen, Ming-Shu Lin & Shao-Yi Hou

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  1. Hsing-Ta Chen
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  2. Ming-Shu Lin
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  3. Shao-Yi Hou
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Correspondence to Shao-Yi Hou.

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Chen, HT., Lin, MS. & Hou, SY. Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production. Korean J. Chem. Eng. 25, 1082–1087 (2008). https://doi.org/10.1007/s11814-008-0177-0

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  • DOI: https://doi.org/10.1007/s11814-008-0177-0

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