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Expression of the B-cell lymphoma/leukemia 11A gene in malignant hematological cell lines through quantitative reverse transcription polymerase chain reaction

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Clinical Oncology and Cancer Research

Abstract

Objective

The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Methods

The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRTPCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydrogenase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA.

Results

The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia (P < 0.05). Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38.

Conclusion

The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.

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Authors and Affiliations

  1. Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, Guangdong Province, China

    Yang-jun Gao, Dong-mei He, Shao-hua Chen, Xiao-juan Yan, Xiao-mao Hu & Yang-qiu Li

  2. Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, Guangdong Province, China

    Yang-qiu Li

Authors
  1. Yang-jun Gao
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  2. Dong-mei He
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  3. Shao-hua Chen
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  4. Xiao-juan Yan
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  5. Xiao-mao Hu
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  6. Yang-qiu Li
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Corresponding author

Correspondence to Dong-mei He.

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Gao, Yj., He, Dm., Chen, Sh. et al. Expression of the B-cell lymphoma/leukemia 11A gene in malignant hematological cell lines through quantitative reverse transcription polymerase chain reaction. Clin. Oncol. Cancer Res. 8, 242–246 (2011). https://doi.org/10.1007/s11805-011-0588-2

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  • DOI: https://doi.org/10.1007/s11805-011-0588-2

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