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Molecularly imprinted polymers for selective extraction of crystal violet from natural seawater coupled with high-performance liquid chromatographic determination

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Abstract

Molecularly imprinted polymers (MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Systematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high affinity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0–200 μg L−1 (R 2 > 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation (n=3) being in the range of 2.89%–5.96%.

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Authors and Affiliations

  1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, P. R. China

    Ziru Lian & Jiangtao Wang

  2. Marine College, Shandong University at Weihai, Weihai, 264209, P. R. China

    Ziru Lian

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  1. Ziru Lian
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  2. Jiangtao Wang
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Correspondence to Jiangtao Wang.

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Lian, Z., Wang, J. Molecularly imprinted polymers for selective extraction of crystal violet from natural seawater coupled with high-performance liquid chromatographic determination. J. Ocean Univ. China 13, 236–242 (2014). https://doi.org/10.1007/s11802-014-2087-6

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  • DOI: https://doi.org/10.1007/s11802-014-2087-6

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