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Isolation and characterization of collagen from squid (Ommastrephes bartrami) skin

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Abstract

Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized. The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type I collagen, containing α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations confirmed the existence of helical arrangements in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts) of PSC were 29.4°C and 52.8°C, respectively.

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China

    Mingyan Yan, Bafang Li & Xue Zhao

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  1. Mingyan Yan
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  2. Bafang Li
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  3. Xue Zhao
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Correspondence to Bafang Li.

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Yan, M., Li, B. & Zhao, X. Isolation and characterization of collagen from squid (Ommastrephes bartrami) skin. J. Ocean Univ. China 8, 191–196 (2009). https://doi.org/10.1007/s11802-009-0191-9

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  • DOI: https://doi.org/10.1007/s11802-009-0191-9

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