Abstract
In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.
Similar content being viewed by others
References
Bitter, T., and Muir, H. M., 1962. A modified uronic acid carbazole reaction. Anal. Chem., 237: 75–80.
Dodgson, K. S., and Price, R. G., 1962. A note on the determination of the ester sulfate content of sulfated polysaccharides. Biochem. J., 84: 106–110.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F., 1956. Colorimetric method for determination of sugers and related substances. Anal. Chem., 28: 350–356.
Feeney, R. E., and Yin, Y., 1998. Antifreeze proteins: Current status and possible food uses. Trends Food Sci. Technol., 9: 102–106.
Filicori, M., Fazleabas, A. T., Huhtaniemi, I., Licht, P., Rao, C., Tesarik, J., et al., 2005. Novel concepts of human chorionic gonadotropin: reproductive system interactions and potential in the management of infertility. Fertil. Steril., 84: 275–284.
Kishino, S., and Miyazaki, K., 2004. Strategy for analysis and screening of bioactive compounds in traditional Chinese medicines. J. Chromatogr. B, 812: 71–84.
Konozy, E. H. E., Bernardes, E. S., Rosa, C., Faca, V., Greene, L. J., and Ward, R. J., 2003. Isolation, purification, and physicochemical characterization of a D-galactose-banding lectin from seeds of Erythrina speciosa. Arch. Biochem. Biophys., 410: 222–229.
Li, C., Yu, H., Liu, S., Xing, R., Guo, Z., and Li, P., 2005. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15: 5370–5374.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951. Protein measurement with the folin-phenol reagent. J. Biol. Chem., 193: 265–275.
Oldfield, D. J., Taylor, M. W., and Singh, H., 2005. Effect of preheating and other process parameters on whey protein reactions during skim milk powder manufacture. Int. Dairy J., 15: 501–511.
Patruce, L. E. P., Mohamed, Z., Hiam, A., and Marie-therese, M., 2000. A glycoprotein isolated from the sponge, pachymatisma johnstionii, has anti-leishmanial activity. Cell Biol. Int., 24: 51–56.
Ryosuke, I., Jun, K., and Masatoshi, Y., 2003. A novel antimicrobial peptide from the sea hare Dolabella auricularia. Dev. Comp. Immunol., 27: 305–311.
Satoshi, K., and Katsumi, M., 1997. Separation methods for glycoprotein analysis and preparation. J. Chromatogr. B, 699: 371–381.
Stumpe, M., Miller, C., Morton, N. S., Bell, G., and Watson, D. G., 2006. High performance liquid chromatography determination of α1-acid glycoprotein in small volumes of plasma from neonates. J. Chromatogr. B., 831: 81–84.
Sun, R. C., Sun, X. F., and Ma, X. H., 2002. Effect of ultrasound on the structural and physiochemical properties of organosolv soluble hemicelluloses from wheat straw. Ultrason. Sonochem., 9: 95–101.
Sun, T., Xu, Z. M., and Godber, J. S., 2006. Ultrasound assisted extraction in quantifying lutein from chicken liver using high-performance liquid chromatography. J. Chromatogr. B., 830: 158–160.
Wakabayashi, H., Yamauchi, K., and Takase, M., 2006. Lactoferrin research, technology and applications. Int. Dairy J., 16: 1241–1251.
Yu, H. H., Li, C. P., Li, R. G., Xing, R. E., Liu, S., and Li, P. C., 2007a. Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye. Food Chem. Toxicol., 45: 1173–1178.
Yu, H. H., Liu, X. G., Dong, X. L., Li, C. P., Xing, R., Liu, S., et al., 2005a. Insecticidal activity of proteinous venom from tentacle of jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15: 4949–4952.
Yu, H. H., Liu, X. G., Xing, R. E., Lui, S., Li, C. P., and Li, P. C., 2005b. Radical scavenging activity of protein from tentacles of jellyfish Rhopilema esculentum. Bioorg. Med. Chem. Lett., 15: 2659–2664.
Yu, H. H., Xing, R. E, Liu, S., Li, C. P., Guo, Z. Y., and Li, P. C., 2007b. Studies on the hemolytic activity of tentacle extracts of jellyfish Rhopilema esculentum Kishinouye: Application of orthogonal test. Int. J. Biol. Macromol., 40: 276–280.
Zhang, W. J., 1999. Glycoconjugates Biochemical Research Technology. Zhejiang University Press, Hangzhou, 362–368.
Zhu, K. X., and Zhou, H. M., 2005. Purification and characterization of a novel glycoprotein from wheat germ water-soluble extracts. Proc. Biochem., 40: 1469–1474.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ren, G., Li, B., Zhao, X. et al. Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography. J. Ocean Univ. China 8, 83–88 (2009). https://doi.org/10.1007/s11802-009-0083-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11802-009-0083-z