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Development study on some digestive enzymes of Takifugu rubripes larvae and juvenile

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Abstract

The activities of some digestive enzymes are studied for Takifugu rubripes larvae and juvenile from the first feeding to 27d after hatching at selected stages of development. The homogenate of whole larvae body is used for enzymatic determination. Activity of acid protease decreases notably during the beginning days after the commencement of completely exogenous feeding and the days before the beginning of the juvenile stage. Alkaline protease specific activity also decreases at metamorphosis. The activities are associated with the morphology of the developing digestive tract. Amylase activity increases before the first feeding, followed by a decreasing and then a rather constant level. Lipase activity remains low during the larvae and juvenile periods. Alkaline phosphatase activity increases gradually. This reflects the development of brush border membranes of enterocytes.

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References

  • Baglole, C.J., G. P. Goff, and G. M. Wrightet, 1998. Distribution and ontogeny of digestive enzymes in larval yellowtail and winter flounder. Journal of Fish Biology, 53: 767–784.

    Article  Google Scholar 

  • Biochemistry Department of Beijing Normal University, 1995. Elementary Biochemistry Experimentation. Higher Education Publishing Company, Beijing, 99–101.

    Google Scholar 

  • Bong, G.K., S. Divakaran, C. K. Brown, and A. C. Ostrowski, 2001. Comparative digestive enzyme ontogeny in two marine larval fishes: Pacific threadfin, Polydactylus sexfilis and bluefin trevally, Caranx melampygus. Fish Physiology and Biochemistry, 24: 225–241.

    Article  Google Scholar 

  • Brown, C.L., and B.G. Kim, 1995. Combined application of cortisol and triiodothyronine in marine finfish culture. Aquaculture, 135(1–3): 79–86.

    Article  Google Scholar 

  • Cahu, C. L., and J. L. Zambonino-Infante, 1994. Early weaning of sea bass (Dicentrarchus labrax) larvae with a compound diet: effect on digestive enzymes. Comp. Biochem Physiol, A109: 213–222.

    Article  Google Scholar 

  • Cahu, C.L., J.L. Zambonino-Infante, A. Peres, P. Quazuguel, and M. M. Le-Gall, 1998. Algal addition in sea bass (Dicentrarchus labrax) larvae rearing: effect on digestive enzymes. Aquaculture, 161: 479–489.

    Article  Google Scholar 

  • Cai, K. X., Wang, C. G., Chen, P. J., and Xie, Y. J., 2000. Ontogenetic development of digestive enzyme activities in larval and juvenile Plectorhynchus cinctus. Journal of Oceanography in Taiwan Strait, 19: 201–204.

    Google Scholar 

  • Cheng, Q.T., Wang, C. X., Tian, M. C., Li, Y.G., and Wang, Y.G., 1975. Studies on the Chinese tetraodonoid fishes of the genus Fugu. Ada Zool Sinica, 21: 359–378.

    Google Scholar 

  • Chen, P.J., Wang, C.G., Huang, C.N., Gu, Y., and Lu, H., 1997. Variation of digestive enzyme activity in different development periods of Pagrosomus major. Journal of Oceanography in Taiwan Strait, 16(3): 245–248.

    Google Scholar 

  • Chen, P.J., and Wang, C.G., 1998. Digestive enzyme activities in larval, juvenile and young Pagrosomus major. Journal of Oceanography in Taiwan Strait, 17(sup): 23–28.

    Google Scholar 

  • Cousin, J. C. B., F. Baudin-Laurencin, and J. Gabaudan, 1987. Ontogeny of enzymatic activities in fed and fasting turbot, Scophthalmus maximus L. Journal of Fish Biology, 30: 13–33.

    Article  Google Scholar 

  • Cong, J.R., Zhang, Z.H., and Yao, S.C., 1998. A study of the histology and morphology of the digestive tract of the juvenile globefish, Fugu rubripes. Article, 4: 53–58.

    Google Scholar 

  • Cuvier-Péres, A., and P. Kestemont, 2002. Development of some digestive enzymes in Eurasian perch larvae Perca fluviatilis. Fish Physiology and Biochemistry, 24: 279–285.

    Article  Google Scholar 

  • Gawlicka, A., L. Mclaughlin, S. S. O. Hung, and J. De La-Noue, 1996. Limitations of carrageenan microbound diets for feeding white sturgeon, Acipenser transmontanus, larvae. Aquaculture, 141: 245–265.

    Article  Google Scholar 

  • Henning, S. T., 1987. Functional development of the gastrointestinal tract. In: Physiology of the Gastrointestinal Tract. 2nd edition. L. R. Johnson, ed., Raven Press, New York, 285–300.

    Google Scholar 

  • Lei, Q.L., Chen, C., Xu, Y. K., Liu, Z. Q., Wang, G. W., et al., 1992. Studies on productive scale seedling rearing of tiger puffer (Fugu rubripes). Marine Fisheries Research, 13: 63–71.

    Google Scholar 

  • Liu, Z. S., Zhou, H. T., Yu, K. K., Sheng, X. Z., and Ma, Y.J., 1994. Studies on the nutrient requirements and mixed feeding of red seabream Pagrus major. Transactions of Oceanology and Limnology, 4: 48–55.

    Google Scholar 

  • Lojda, Z., R. Gossrau, and T.H. Schiebler, 1979. Enzyme Histochemistry:. A Laboratory Mannal. Springer-Verlag Berlin Heidelberg, New York, 399 pp.

    Google Scholar 

  • Moyano, F. J., M. Diaz, F. J. Alarcon, and M. C. Sarasquete, 1996. Characterization of digestive enzyme activity during larval development of gilthe seabream (Spams aurata). Fish Physical. Biochem, 15: 121–130.

    Article  Google Scholar 

  • Oozeki, Y., and K.M. Bailey, 1995. Ontogenetic development of digestive enzyme activities in larval walleye Pollock, Theragra chalcqgramma. Marine Biology, 122: 177–186.

    Google Scholar 

  • Pan, L. Q., and Wang, K. X., 1997. The experimental studies on activities of digestive enzyme in the larvae Penaeus chinensis. Journal of Fisheries of China, 21(1): 26–31.

    Google Scholar 

  • Ribeiro, L., J. L. Zambonino-Infante, C. Cahu, and M. T. Dinis, 1999. Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858. Aquaculture, 179: 465–473.

    Article  Google Scholar 

  • Segner, H., R. Rosch, H. Schmidt, and K.J. Von Poeppinghausen, 1989. Digestive enzymes in larval Coregonus lavaretus L. Journal of Fish Biology, 35: 249–263.

    Article  Google Scholar 

  • Segner, H., V. Storch, M. Reinecke, W. Kloas, and W. Hanke, 1994. The development of functional digestive and metabolic organs in turbot Scophthalmus maximus. Mar. Biol., 119: 471–486.

    Article  Google Scholar 

  • Sun, Z. Z., 2002. Studies on the Biological characteristic and artificial seed-rearing technique of Fugu rubripes. Shangdong Fisheries, 19(8): 44–46.

    Google Scholar 

  • Tabeta, O., 1997. Fisheries and Stock Management of Ocellate Puffer Takifugu rubripes in Japan. Koseisa, Tokyo, 16–17.

    Google Scholar 

  • Walford, J., and T.J. Lam, 1993. Development of digestive tract and proteolytic enzyme activity in sea bass (Late calcarifer) larvae and juveniles. Aquaculture, 109: 187–205.

    Article  Google Scholar 

  • Wang, K.Q., Chen, M., and Gao, T.X., 2001. Study on taxonomy and fauna of Takifugu. Journal of Ocean University of Qingdao, 31(6): 855–860.

    Google Scholar 

  • Zambonino-Infante, J.L., and C.L. Cahu, 2001. Ontogeny of the gastrointestinal tract of marine fish larvae. Comparative Biochemistry and Physiology Part C, 130: 477–487.

    Article  Google Scholar 

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Correspondence to Gao Tianxiang.

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Wan, Z., Gao, T., Zhang, X. et al. Development study on some digestive enzymes of Takifugu rubripes larvae and juvenile. J Ocean Univ. China 3, 175–178 (2004). https://doi.org/10.1007/s11802-004-0031-x

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  • DOI: https://doi.org/10.1007/s11802-004-0031-x

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