Abstract
The efficacy of ultraviolet (UV) disinfection has been analyzed and validated by numerous studies using culture-based methods, yet the discovery of the viable but nonculturable state necessitates the investigation of UV disinfection based on viability parameters. Paired regulators of the SOS response system, recA-lexA, and the programmed cell death system, mazEF, in Escherichia coli were chosen as the target genes, and the effect of UV irradiation on the mRNAs of the four genes was studied. This research showed that, after UV irradiation, the responses of the mRNAs were highly consistent, with reduction percentages of approximately 60% at 20 mJ/cm2, 70% at 40 mJ/cm2, and 90% at 80 mJ/cm2, and these reductions were believed to be the result of direct UV damage to nucleic acids. After 24 h of dark incubation, recA and lexA were both upregulated but to a lesser extent for repressor lexA; and mazE and mazF were both downregulated. This result implies that UV irradiation induces the dark repair system more actively, and the cells will proceed to death at a rate similar to that associated with natural decay.
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Acknowledgements
This research was supported by the Chinese Major National R&D Project (Nos. 2017ZX07108-003 and 2017ZX07502003).We thank Professor Karl Linden and his group from University of Colorado, Boulder for providing enormous help in experimental support and technique instruction. We thank Professor Wenjun Liu from Tsinghua University who, although no longer with us, had inspired and guided us the students to dedicate to this study and the career that he loved in public health.
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Yang, C., Sun, W. & Ao, X. Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of E. coli. Front. Environ. Sci. Eng. 13, 26 (2019). https://doi.org/10.1007/s11783-019-1111-x
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DOI: https://doi.org/10.1007/s11783-019-1111-x