Abstract
Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supernatant of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50×104. Its maximum activity appears at pH 5 and 55 °C. The chitinase is stable at pH 4.0–7.5 and below 45 °C.
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Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China
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Xia, Jl., Xiong, J., Xu, T. et al. Purification and characterization of extracellular chitinase from a novel strain Aspergillus fumigatus CS-01. J. Cent. South Univ. Technol. 16, 552–557 (2009). https://doi.org/10.1007/s11771-009-0092-5
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DOI: https://doi.org/10.1007/s11771-009-0092-5