Abstract
A bacterial strain that was capable of degrading organic sulfur (dibenzothiophene) was isolated by enrichment techniques from the petroleum-contaminated soil collected from Zhongyuan Oil Field. The strain is named ZYX and is gram-positive. This strain undergoes bacilus-coccus morphological change, and forms yellow-pigment glossy circular colonies with 1.5 mm in diameter on average after 2 d incubation on Luria-Bertani(LB) plates. The full-length of 16S rDNA sequence of strain ZYX was determined and analyzed. Strain ZYX is found most relative with the genus of Arthrobacter. The similarity values between ZYX and Arthrobacter sp. P2 is 99.53%. The main morphological, biochemical and physiological features of strain ZYX accord with those of Arthrobacter. It is found that the optimal initial pH for growth is about 7.0, and the optimal concentration of dibenzothiophene(DBT) for growth is 0.10 g/L. Additionally, the results show that the best carbon source and nitrogen source are glycerol and glutamine, respectively.
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WANG Zhi-xuan, PAN Li, PENG Jun. Analysis of current status, cost and policies of power sector SO2 emission control[J]. Research of Environmental Sciences, 2005, 18(4): 11–20. (in Chinese)
LEI Fang-ming. Review of technology on fuel coal sulphur removal[J]. Energy Conservation, 2002, 243(10): 16–18. (in Chinese)
QIU Jian-hui, DI Jin-shen, LI Ying-jie. Progress of biodesulfurization[J]. Acta Microbiologica Sinica, 2001, 41(5): 650–653. (in Chinese)
DEL OLMO C H, SANTOS V E, ALCON A, et al. Production of a Rhodococcus erythropolis IGTS8 biocatalyst for DBT biodesulfurization: Influence of operational conditions[J]. Biochemical Engineering Journal, 2005, 22: 229–237.
SUN Li-mei, SHAN Zhong-jian. Research progress of coal desulfurization at host combustion in domestic and abroad[J]. Clean Coal Technology, 2005, 11: 55–59. (in Chinese)
ZHOU Zhi-fu, WEI De-zhou, WANG Ying-min, et al. The progress of the research on microbial pretreatment and floatation desulphurization in coal[J]. Industrial Safety and Environmental Protection, 2002, 28(2): 3–7. (in Chinese)
CHENG Sheng-gao, WANG Yan-lin. Stored-up status of organic sulfur in high sulfuric coal and desulfurized mechanism with microbe[J]. China Environmental Protection Industry, 1996, 12: 34–35. (in Chinese)
DAHLBERG M D, ROHRER R L, FAUTH D J, et al. Biodesulfurization of dibenzothiophene sulfone by Arthrobacter sp. and studies with oxidized Illinois[J]. 1993, 72: 1645–1649.
HOU Zhong-xuan, LIU Hui-zhou, LUO Ming-fang, et al. Isolation and characterization of a desulfurization bacterium[J]. Science in China (B), 2002, 32(5): 397–405. (in Chinese)
SHEN Ping, FAN Xiu-rong, LI Guang-wu, et al. Microbiological Experiments[M]. The Third Edition. Beijing: Higher Education Press, 1999.
XU Wei-chang. Application of Biotechnology in Nuclear Industry[M]. Hunan: National University of Defense Technology Press, 2002. (in Chinese)
THOMPSON J D, GIBSON T J, PLEWNIAK F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research, 1997, 24: 4876–4882.
KEDDIE R M, COLLINS D, JONES D, SNEATH P H A, MAIR N S, SHARPE M E, Holt J G (eds). (1986a) Genus Arthrobacter. Bergey’s Manual of Systematic Bacteriology(Vol.2)[M]. Baltimore: Williams & Wilkins, 1986: 1288–1301.
Microorganism Institute, The Chinese Academy of Sciences. Common Identification Technique of Normal Bacteria[M]. Beijing: Science Press, 1984. (in Chinese)
CAROLINA H D, ALMUDENA A, VICTORIA E S, et al. Modeling the production of a Rhodococcus erythropolis IGTS8 biocatalyst for DBT biodesulfurization: Influence of media composition[J]. Enzyme and Microbial Technology, 2005, 37: 157–166.
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Foundation item: Project(50621063) supported by the National Natural Science Foundation of China; Project(05JJ30023) supportecd by Natural Science Foundation of Hunan Province, China
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Yang, Y., Diao, Mx., Shi, Wy. et al. Isolation and characterization of organic-sulfur degradation bacterial strain. J Cent. South Univ. Technol. 14, 324–329 (2007). https://doi.org/10.1007/s11771-007-0064-6
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DOI: https://doi.org/10.1007/s11771-007-0064-6