Abstract
Acephate is an organophosphorus pesticide used against sucking and biting insects. Although it is not recommended, its residues are found in berries at the time of harvest. The study aimed to explore and identify the bacteria from the grape berry surface which could be applied in the bioremediation of acephate. Accordingly, bacteria AC3 was isolated from the grape berry surface. The bacterium was identified as Kocuria sediminis after 16 S rDNA gene area sequencing. The LC-MS/MS analysis showed that K. sediminis degraded acephate by 61.6% at the concentration of 1ppm, in 7 days. It also showed effective plant growth-promoting activity. The productivity of indole acetic acid (IAA) by K. sediminis after four days of incubation was 57. 60 µg/mL. The present study is the first report that confirmed the role of epiphytic microbes, present on grape berry surface, in the detoxification of pesticides with plant growth-promoting activity.
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Abbreviations
- IAA:
-
Indole acetic acid.
- MSM:
-
Mineral salt medium.
- MSA:
-
Mineral salt agar.
- SDW:
-
Sterile distilled water.
- NB:
-
Nutrient broth.
- MIC:
-
Minimum Inhibitory Concentration.
- PGPR:
-
Plant growth-promoting rhizobacteria.
- CFU:
-
Colony-forming unit.
- MRM:
-
Multiple reaction monitoring.
- RF:
-
Retention factor.
- NCBI:
-
National Centre for Biotechnology Information.
- BEH:
-
Bridged ethylene hybrid.
- UHPLC:
-
Ultra-high-performance liquid chromatography.
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Acknowledgements
The authors would like to thank the Director, ICAR- National Research Centre for Grapes, the Director and Head of school, MIT School of Bioengineering Sciences and Research for their support during the study.
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Y.R and R.S carried out experimental work. Y.R, S.S, M.C, I.S, and P.P jointly prepared the manuscript. Y.R, S.S, M.C, I.S, R.S, and P.P actively participated in the discussion and revision of the manuscript.
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Ranade, Y.H., Saha, S., Chandrashekar, M. et al. Functionality of fructosphere bacteria Kocuria sediminis strain AC3 in plant growth promotion and biodegradation of the acephate in grape berries. Biologia 77, 3255–3263 (2022). https://doi.org/10.1007/s11756-022-01171-y
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DOI: https://doi.org/10.1007/s11756-022-01171-y