Abstract
The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families involved in transporting auxin out of the cell and regulating intracellular auxin homeostasis. However, the tissue-specific expression profiles of OsPIN genes in rice (Oryza sativa) and their expression patterns under abiotic stress and exogenous hormone treatments remain unclear. In this study, a comprehensive tissue-specific expression analysis of OsPIN genes was performed, and the responses of OsPIN genes to diverse abiotic stress (drought, salt and cold) and hormone treatments (abscisic acid, ABA; gibberellin, GA; methyl jasmonate, MeJA and salicylic acid, SA) were further investigated. Six OsPIN genes (OsPIN1a, OsPIN1b, OsPIN5a, OsPIN5b, OsPIN9, and OsPIN10a) showed ubiquitous expression at the seedling and filling stage. Two OsPIN genes (OsPIN2 and OsPIN5c) were expressed predominantly in seedling roots, four OsPIN genes (OsPIN1c, OsPIN1d, OsPIN8 and OsPIN10b) were higher expressed in root-shoot junctions, indicating that OsPIN genes are functionally diverse. OsPIN5b and OsPIN9 were found to respond to all abiotic stress and hormone treatments dramatically in rice seedling roots, indicating that they are potentially involved in the regulation of abiotic stress and hormone signaling to balance plant growth and various exogenous stimuli by guiding auxin flux. Our results provide fundamental insights into the potential regulatory role of OsPIN genes in different tissues and responses to abiotic stress and hormones.
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Data availability
All data generated or analyzed during this study are included in this published article.
Abbreviations
- PAT:
-
Polar auxin transport
- PIN:
-
PIN-FORMED
- PM:
-
Plasma membrane
- ER:
-
Endoplasmic reticulum
- RT-qPCR:
-
quantitative RT-PCR
- RPKM:
-
Reads/Kb/Million
- ABA:
-
Abscisic acid
- GA:
-
Gibberellin
- MeJA:
-
Methyl jasmonate
- SA:
-
Salicylic acid
- IAA:
-
Indole-3-acetic acid
- 6-BA:
-
6-benzylaminopurine
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- JA:
-
Jasmonic acid
- BR:
-
Brassinolide
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This research was funded by the National Natural Science Foundation of China (grant number 3110019), the Natural Science Foundation of Henan Province in China (grant number 182300410012 and 202300410151) and the State Key Laboratory of Cotton Biology Open Fund (grant number CB2020A24).
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Conceptualization: Xu Huawei and Hou Dianyun; Investigation: Zhang Yanwen, Yang Xiaoyi and Wang Huihui; Writing-original draft preparation: Xu Huawei; Writing-review and editing: Xu Huawei and Hou Dianyun. All authors have read and agreed to the published version of the manuscript.
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Fig. S1
Gene structures of OsPIN genes. (PNG 110 kb)
Fig. S2
Prediction of the transmembrane helices in OsPIN proteins with TMHMM Server v. 2.0. (PNG 98 kb)
Fig. S3
Relative expression of OsPIN genes in the same tissue. (PNG 178 kb)
Fig. S4
Cis-element analysis of putative OsPIN promoters related tostress and hormone responses. Cis-elements response to the same stimuli are displayed in the same colour. (PNG 178 kb)
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Xu, H., Zhang, Y., Yang, X. et al. Tissue specificity and responses to abiotic stresses and hormones of PIN genes in rice. Biologia 77, 1459–1470 (2022). https://doi.org/10.1007/s11756-022-01031-9
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DOI: https://doi.org/10.1007/s11756-022-01031-9