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Production of 8,11-cis-eicosadienoic acid by a Δ5 and Δ12 desaturase-defective mutant derived from the arachidonic acid-producing fungus Mortierella alpina 1S-4

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Journal of the American Oil Chemists' Society

Abstract

The Δ5 and Δ12 desaturase (DS)-blocked mutants of an arachidonic acid-producing fungus, Mortierella alpina 1S-4, were obtained. These mutants accumulated 8,11-cis-eicosadienoic acid (20:2n-9). One of the mutants, M226-9, in which Δ5 and Δ12 DS are perfectly blocked, produced 1.68 mg of 20:2n-9 per mL of culture medium (101 mg/g dry mycelia) and no 5,8,11-cis-eicosatrienoic acid (20:3n-9) when grown in a medium containing 4% glucose and 1% yeast extract at 28°C for 2 d and then at 12°C for 12 d. The mycelial lipid comprised 77.4% triacylglycerol (TG) and 9.8% phosphatidylcholine (PC), among others. TG contained 69.0% of the total 20:2n-9, whose percentage in total TG fatty acids was 15.9%. The highest percentage (44.4%) of 20:2n-9 was found in PC. The addition of olive oil to the culture medium enhanced the production of 20:2n-9.

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Author notes

  1. Hiroshi Kawashima & Kengo Akimoto

    Present address: Consumer Health Products Development Department, Institute for Eundamental Research, Suntory, Ltd., Shimamoto-cho, Mishimagun, 618-0001, Osaka, Japan

Authors and Affiliations

  1. Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, 606-8502, Kyoto, Japan

    Nozomu Kamada, Hiroshi Kawashima, Eiji Sakuradani, Kengo Akimoto, Jun Ogawa & Sakayu Shimizu

Authors
  1. Nozomu Kamada
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  2. Hiroshi Kawashima
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  3. Eiji Sakuradani
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  4. Kengo Akimoto
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  5. Jun Ogawa
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  6. Sakayu Shimizu
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Correspondence to Sakayu Shimizu.

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Kamada, N., Kawashima, H., Sakuradani, E. et al. Production of 8,11-cis-eicosadienoic acid by a Δ5 and Δ12 desaturase-defective mutant derived from the arachidonic acid-producing fungus Mortierella alpina 1S-4. J Amer Oil Chem Soc 76, 1269–1274 (1999). https://doi.org/10.1007/s11746-999-0138-8

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  • DOI: https://doi.org/10.1007/s11746-999-0138-8

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