Abstract
Enzymatic synthesis of acyglycerols directly from glycerol and an θ-3 fatty acid concentrate, prepared from seal blubber oil, in organic solvents was studied. Seven lipases were used as biocatalysts for esterification, Lipase LP-401-AS from Chromobacterium viscosum showed the highest activity for esterification. Effects of reaction parameters, namely, temperature, time course, type of solvent, water content, amount of glycerol, enzyme load and solvent volume, were followed with lipase IP-401-AS as the biocatalyst of choice. Optimal reaction conditions were established, and the maximal degree of acylglycerol synthesis reached was 94.3%. The concentrations of monoacylglycerols, diacylglycerols, and triacylglycerols were 13.8, 43.1, and 37.4%, respectively. Therefore, acylglycerols containing predominantly θ-3 fatty acid concentrates may be easily synthesized directly via their reaction with glycerol.
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References
Johnston, P., and J.E. Hunter, Perspectives on Omega-3 Fatty Acids, J. Am. Oil Chem. Soc. 64:716–717 (1987).
Stansby, M.E., Marine-Derived Fatty Acids or Fish Oils as Raw Material for Fatty Acids Manufacture, Ibid.:793A-796A (1979).
Ackman, R.G., Oils and Fats Group International Lecture: The Year of the Fish Oils, Chemistry and Industry 7:139–145 (1988).
Lawson, L.D., and B.G. Hughes, Human Absorption of Fish Oil Fatty Acids as Triacylglycerols, Free Acids, or Ethyl Esters, Biochem. Biophys. 152:328–335 (1988).
Ackman, R.G., and W.M.N. Ratnayake, Chemical and Analytical Aspects of Assuring an Effective Supply of Omega-3 Fatty Acids to the Consumer, in Omega-3 Fatty Acids in Health and Disease, edited by R.S. Lees and M. Karel, Marcel Dekker Inc., New York, 1990, pp. 215–234.
Ackman, R.G., n-s News, Unsaturated Fatty Acids and Health 14:1–3 (1986).
Osada, K., K. Takahashi, and M. Hatano, Modification of Sardine Oil by Immobilized Bacterial Lipase, J. Jpn. Oil Chem. Soc. 39:467–471 (1990).
Huang, K.-H., and C.C. Akoh, Lipase-Catalyzed Incorporation of n-3 Polyunsaturated Fatty Acids into Vegetable Oils, J. Am. Oil Chem. Soc. 71:1277–1280 (1994).
Wanasundara, U.N., and F. Shahidi, Storage Stability of Microencapsulated Seal Blubber Oil, J. Food Lipids 2:73–86 (1995).
Haagsma, N., C.M. Van Gent, J.B. Luten, R.W. De Jong, and E. Van Doorn, Preparation of an θ-3 Fatty Acids Concentrate from Cod Liver Oil, J. Am. Oil Chem. Soc. 59:117–118 (1982).
Li, Z.-Y., and D.P. Ward, Lipase-Catalyzed Esterification of Glycerol and n-3 Polyunsaturated Fatty Acid Concentrate in Organic Solvent, Ibid.:745–748 (1993).
Dordick, J.S., Enzymic Catalysis in Monophasic Organic Solvents, Enzyme Microb. Technol. 11:194–198 (1989).
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He, Y., Shahidi, F. Enzymatic esterification of θ-3 fatty acid concentrates from seal blubber oil with glycerol. J Amer Oil Chem Soc 74, 1133–1136 (1997). https://doi.org/10.1007/s11746-997-0036-x
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DOI: https://doi.org/10.1007/s11746-997-0036-x