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Simultaneous Enzymatic Transesterification and Esterification of an Acid Oil Using Fermented Solid as Biocatalyst

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

This work studied the enzymatic synthesis of fatty acid ethyl esters (FAEE) for potential use as biodiesel via simultaneous esterification and transesterification of acid oil from macaúba in a solvent-free system. A fermented and dry babassu cake with lipase activity from Rhizomucor miehei was used as biocatalyst. FAEE content above 85% was achieved after 96 h of reaction with enzyme loading of 13 U per g of oil, 120 mmol of hydrous ethanol (95% ethanol and 5% water)/20 mmol of oil (molar ratio ethanol:oil of 6:1), at 40 °C. After two consecutive enzymatic reactions, 90.8 wt% FAEE content was obtained. These results demonstrate a promising transesterification/esterification method for FAEE production from an acid and low-cost oil and the process has potential to decrease the costs of enzymatic biodiesel production.

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Acknowledgements

This research received financial support from FAPERJ, CNPq, ANP, and PETROBRAS. The authors are also grateful to Dr. Donato Alexandre Gomes Aranda, of the “Laboratory of Green Technologies (GREENTEC)” located in Federal University of Rio de Janeiro, to kindly provide his laboratory for biodiesel analyses.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Rio De Janeiro, RJ, CEP 21949-909, Brazil

    Erika C. G. Aguieiras, Elisa D. Cavalcanti-Oliveira & Denise M. G. Freire

  2. PETROBRAS/CENPES/PDEDS/Biotecnologia, Ilha do Fundão, Av. Horácio Macedo, 950, Rio De Janeiro, RJ, CEP 21941-915, Brazil

    Aline M. de Castro

  3. Departamento de Química Analítica, Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524,PHLC, sl. 310, Rio De Janeiro, RJ, CEP 20550-013, Brazil

    Marta A. P. Langone

Authors
  1. Erika C. G. Aguieiras
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  2. Elisa D. Cavalcanti-Oliveira
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  3. Aline M. de Castro
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  4. Marta A. P. Langone
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  5. Denise M. G. Freire
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Corresponding authors

Correspondence to Erika C. G. Aguieiras or Denise M. G. Freire.

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Aguieiras, E.C.G., Cavalcanti-Oliveira, E.D., de Castro, A.M. et al. Simultaneous Enzymatic Transesterification and Esterification of an Acid Oil Using Fermented Solid as Biocatalyst. J Am Oil Chem Soc 94, 551–558 (2017). https://doi.org/10.1007/s11746-017-2964-4

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  • DOI: https://doi.org/10.1007/s11746-017-2964-4

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