Skip to main content
Log in

Characterization of Oxidative Stability of Fish Oil- and Plant Oil-Enriched Skimmed Milk

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

The objective of this research was to determine the oxidative stability of fish oil blended with crude plant oils rich in naturally occurring antioxidants, camelina oil and oat oil, respectively, in bulk and after supplementation of 1 wt% of oil blends to skimmed milk emulsions. Ability of crude oat oil and camelina oil to protect fish oil in bulk and as fish oil-enriched skimmed milk emulsions was evaluated. Results of oxidative stability of bulk oils and blends assessed by the Schaal oven weight gain test and by the rancimat method showed significant increase in oxidative stability when oat oil was added to fish oil in only 5 and 10 %, whereas no protective effect of camelina oil was observed when evaluated by these methods. Moreover, fish oil blended with oat oil conferred the lowest PV and lower amounts of volatile compounds during the storage period of 14 days at 4 °C. Surprisingly, skimmed milk supplemented with fish-oat oil blend gave the highest scores for off-flavors in the sensory evaluation, demonstrating that several methods, including sensory analysis, should be combined to illustrate the complete picture of lipid oxidation in emulsions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  1. Simopoulos AP (1999) Essential fatty acids in health and chronic disease. Am J Clin Nutr 70:560–569

    Google Scholar 

  2. Ruxton CHS, Reed SC, Simpson MJA, Millington KJ (2007) The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence. J Nutr Diet 20:275–285

    Article  CAS  Google Scholar 

  3. Trautwein EA (2001) n-3 Fatty acids—physiological and technical aspects for their use in food. Eur J Lipid Sci Technol 103:45–55

    Article  CAS  Google Scholar 

  4. Frankel EN (2005) Lipid oxidation, 2nd edn. The oily press, England

    Google Scholar 

  5. Coupland JN, McClements DJ (1996) Lipid oxidation in food emulsions. Trends Food Sci Technol 7:83–91

    Article  CAS  Google Scholar 

  6. Mei L, McClements DJ, Decker EA (1999) Lipid oxidation in emulsions as affected by charge status of antioxidants and emulsion droplets. J Agric Food Chem 47:2267–2273

    Article  CAS  Google Scholar 

  7. Nielsen NS, Klein A, Jacobsen C (2009) Effect of ingredients on oxidative stability of fish oil-enriched drinking yoghurt. Eur J Lipid Sci Technol 111:337–345

    Article  CAS  Google Scholar 

  8. Nicovska K (2010) Oxidative stability and rheological properties of oil-in-water emulsions with walnut oil. Adv J Food Sci Technol 2:172–177

    Google Scholar 

  9. Dauqan EMA, Abdullah A, Sani AH (2011) Natural antioxidants, lipid profile, lipid peroxidation, antioxidant enzymes of different vegetable oils. Adv J Food Sci Technol 3:308–316

    CAS  Google Scholar 

  10. Abramovic H, Butinar B, Nikolicˇ V (2007) Changes occurring in phenolic content, tocopherol composition and oxidative stability of camelina sativa oil during storage. Food Chem 104:903–909

    Article  CAS  Google Scholar 

  11. Peterson DM (2001) Oat antioxidants. J Cer Sci 33:115–129

    Article  CAS  Google Scholar 

  12. Padely FB, Freeman, IP, Polman, RG, vanLookern GJ (1989) European patent 304 115

  13. Eidhin D, O’Beirne D (2010) Oxidative stability and acceptability of camelina oil blended with selected fish oils. Eur J Lipid Sci Technol 112:878–886

    Article  CAS  Google Scholar 

  14. American Oil Chemists’ Society (AOCS) (2009) Official methods and recommended practices of the American oil chemists’ society. Method Cd 8b–90. AOCS Press, Champaign

    Google Scholar 

  15. American Oil Chemists’ Society (AOCS) (1997) Official methods and recommended practices of the American oil chemists’ society. Method Ch 1–91. AOCS Press, Champaign

    Google Scholar 

  16. American Oil Chemists’ Society (AOCS) (1992) Official methods and recommended practices of the American oil chemists’ society. Method Ce 1b–89. AOCS Press, Champaign

    Google Scholar 

  17. Panfili G, Fratianni A, Irano M (2003) Normal phase high-performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereals. J Agric Food Chem 51:3940–3944

    Article  CAS  Google Scholar 

  18. American Oil Chemists’ Society (AOCS) (1993) Official methods and recommended practices of the American oil chemists’ society. Method Cd 12b–92. AOCS Press, Champaign

    Google Scholar 

  19. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–991

    Article  CAS  Google Scholar 

  20. Iverson SJ, Lang SLC, Cooper MH (2001) Comparison of the Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue. Lipids 36:1283–1287

    Article  CAS  Google Scholar 

  21. International Dairy Federation (1991) International IDF Standards, IDF-Square Vergote 41, Brussels, Belgium. section 74A

  22. Timm-Heinrich M, Xu XB, Nielsen NS, Jacobsen C (2003) Oxidative stability of milk drinks containing structured lipids produced from sunflower oil and caprylic acid. Eur J Lipid Sci Technol 2003(105):459–470

    Article  Google Scholar 

  23. Hartvigsen K (2000) Dynamic headspace gas chromatography/mass spectrometry characterization of volatiles produced in fish oil enriched mayonnaise during storage. J Agric Food Chem 48:4858–4867

    Article  CAS  Google Scholar 

  24. Let MB, Jacobsen C, Frankel EN, Meyer AS (2003) Oxidative flavour deterioration of fish oil enriched milk. Eur J Lipid Sci Technol 105:518–528

    Article  CAS  Google Scholar 

  25. Brindzova L, Certik M, Rapta P, Zalibera M, Mikulajova A, Takacsova M (2008) Antioxidant activity, beta-glucan and lipid contents of oat varieties. Czech J Food Sci 26:163–173

    CAS  Google Scholar 

  26. Forssell P, Kervinen R, Alkio M, Poutanan K (1992) Comparison of methods for separating polar lipids from oat oil. Fett Wiss Technol 94:355–358

    CAS  Google Scholar 

  27. Kamal-Eldin A, Appelqvist L (1996) The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 31:671–701

    Article  CAS  Google Scholar 

  28. Kaukonen O, Zock PL, Mensik RP (2011) Foaming of differently processed oats: role of nonpolar lipids and tryptophanin proteins. Cer Chem 88:239–244

    Article  CAS  Google Scholar 

  29. Méndez E, Sanhueza J, Speisky H, Valenzuela A (1996) Validation of the rancimat test for the assessment of the relative stability of fish oils. J Am Oil Chem Soc 73:1033–1037

    Article  Google Scholar 

  30. Let MB, Jacobsen C, Meyer AS (2004) Effects of fish oil type, lipid antioxidants and presence of rapeseed oil on oxidative flavour stability of fish oil enriched milk. Eur J Lipid Sci Technol 106:170–182

    Article  CAS  Google Scholar 

  31. Jacobsen C, Let MB, Nielsen NS, Meyer AS (2008) Antioxidant strategies for preventing oxidative flavour deterioration of foods enriched with n-3 polyunsaturated lipids: a comparative evaluation. Trends Food Sci Technol 19:76–93

    Article  CAS  Google Scholar 

  32. Heinio RL, Lehtinen P, Oksman-Caldentey KM, Poutanen K (2002) Differences between sensory profiles and development of rancidity during ling term storage of native and processed oat. Cereal Chem 79:367–375

    Article  CAS  Google Scholar 

  33. Saga LC, Rukke EO, Liland KH, Kirkhus B, Egelandsdal B, Karlsen J, Volden J (2011) Oxidative stability of polyunsaturated edible oils mixed with microcrystalline cellulose. J Am Oil Chem Soc 88:1883–1895

    Article  CAS  Google Scholar 

  34. Waraho T, Cardiena V, Rodrigues-Estrada MT, McClements DJ, Decker EA (2009) Prooxidant mechanisms of free fatty acids in stripped soybean oil-in-water emulsions. J Agric Food Chem 57:7112–7117

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Denomega A/S, Bioforsk and Swedish oat fiber AB for providing the oils used in this study.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Linda C. Saga.

About this article

Cite this article

Saga, L.C., Kristinova, V., Kirkhus, B. et al. Characterization of Oxidative Stability of Fish Oil- and Plant Oil-Enriched Skimmed Milk. J Am Oil Chem Soc 90, 113–122 (2013). https://doi.org/10.1007/s11746-012-2148-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-012-2148-1

Keywords

Navigation