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Characterization of Fatty Oil of Zizyphi spinosi semen Obtained by Supercritical Fluid Extraction

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

Zizyphi spinosi semen (ZSS) has been widely used for treatment of insomnia in oriental countries. The aim of this study is to characterize the fatty oil of ZSS obtained by supercritical fluid extraction in terms of chemical composition and physicochemical properties. The chemical composition, including fatty acids and unsaponifiable constituents, was analyzed by gas chromatography–mass spectrometer (GC–MS). The results revealed that 9-octadecenoic acid (43.38 ± 0.03%) and 9,12-octadecadienoic acid (40.58 ± 0.03%) were the main fatty acids, and β-sitosterol (37.39 ± 0.02%) and squalene (30.79 ± 0.01%) were the key unsaponifiables. Furthermore, four indexes were assayed according to Chinese Pharmacopeia (2005) to reflect the physicochemical properties of ZSS oil, their values being determined as follows: acid value (10.3 ± 0.1 mg KOH/g), peroxide value (0.05 ± 0.01 g/100 g), saponification value (194.4 ± 0.5 mg KOH/g) and iodine value (109.7 ± 0.8 g I/100 g). The basic information obtained provides data support for quality evaluation and efficacy research of ZSS oil, and suggests its prospects for development in pharmaceutical and food industries.

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References

  1. Han YN, Hwang KH, Han BH (2005) Inhibition of calmodulin-dependent protein kinase II by cyclic and linear peptide alkaloids from Zizyphus species. Arch Pharmacal Res 28:159–163

    Article  CAS  Google Scholar 

  2. Park JH, Lee HJ, Koh SB, Ban JY, Seong YH (2004) Protection of NMDA-induced neuronal cell damage bymethanol extract of Zizyphi Spinosi Semen in cultured rat cerebellar granule cells. J Ethnopharmacol 95:39–45

    Article  Google Scholar 

  3. Zhao J, Li SP, Yang FQ, Li P, Wang YT (2006) Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction. J Chromatogr A 1108:188–194

    Article  CAS  Google Scholar 

  4. Ha NY, Kim SW, Park JK (2009) Pharmaceutical composition for use as health food for treating vascular dementia and alzheimer dementia, comprises ginsenoside-F2, ginsenoside-CK as main component and component of ginseng. KR2009083780-A

  5. Kim WY (2004) Health supplementary food effective for removal of stress and production method. KR2004006705-A

  6. Jo CW, Jo JS, Cho HS, Kim KH (2005) Health supplementary food for preventing and lowing hypertension containing several medicinal herbs having effect of controlling blood pressure. KR2005121815-A

  7. Zhang ST (2005) Health food for promoting sleep contains Zizyphi Spinosi Semen, Biota orientalis, Albizia julibrissin skin, Polygonum multiflorum, Valeriana and/or Menyanthes trifoliate as raw material. JP2005060357-A

  8. Zhao QX, Wang QL, Huang JH (1995) Effect of Zizyphi Spinosi Semen oil on the central nervous system of mice. J Xi’an Med Univ 16:432–434

    Google Scholar 

  9. Heo HJ, Park YJ, Suh YM (2003) Effects of oleamide on choline acetyltransferase and cognitive activities. Biosci Biotechnol Biochem 67:1284–1291

    Article  CAS  Google Scholar 

  10. Wu SL, Yuan BX, Ma ZY (2001) Effect of the long-time use of Zizyphi Spinosi Semen oil to hypnotic effect to mice. Xibei Pharm Mag 16:114–115

    Google Scholar 

  11. Porto CD, Decorti D, Kikic I (2009) Flavour compounds of Lavandula angustifolia L. to use in food manufacturing: comparison of three different extraction methods. Food Chem 112:1072–1078

    Article  Google Scholar 

  12. Chan KW, Ismail M (2009) Supercritical carbon dioxide fluid extraction of Hibiscus cannabinus seed oil: a potential solvent-free and high antioxidative edible oil. Food Chem 114:970–975

    Article  CAS  Google Scholar 

  13. Bernardo-Gil MG, Casquilho M, Esquivel MM, Ribeiro MA (2009) Supercritical fluid extraction of fig leaf gourd seeds oil: fatty acids composition and extraction kinetics. J Supercrit Fluids 49:32–36

    Article  CAS  Google Scholar 

  14. Seal CE, Kranner I, Pritchard HW (2008) Quantification of seed oil from species with varying oil content using supercritical fluid extraction. Phytochem Ana 19:493–498

    Article  CAS  Google Scholar 

  15. Shao P, Sun PL, Ying YJ (2008) Response surface optimization of wheat germ oil yield by supercritical carbon dioxide extraction. Food Bioprod Process 86:227–231

    Article  CAS  Google Scholar 

  16. Kou XY, Yu GP (2006) Researches on the methyl method of fat and fatty acid. Food Res Dev 26:46–47

    Google Scholar 

  17. Shi QR, Zhou G, Zhou P, Zhang WD (2004) Research summary on the traditional Chinese herb Zizyphi Spinosi Semen. J Pharm Pract 22:94–97

    Google Scholar 

  18. Simopoulos AP (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56:365–379

    Article  CAS  Google Scholar 

  19. Schierf G, Oster P, Heuck CC, Raetzer H, Schellenberg B (1978) Sitosterol in juvenile type II hyperlipoproteinemia. Atherosclerosis 30:245–248

    Article  Google Scholar 

  20. Nieminen P, Polonen I, Mustonen AM (2010) Increased reproductive success in the white American mink (Neovison vison) with chronic dietary β-sitosterol supplement. Anim Reprod Sci 119:287–292

    Article  CAS  Google Scholar 

  21. Aioi A, Shimizu T, Kuriyama K (1995) Effect of squalene on superoxide anion generation induced by a skin irritant, lauroylsarcosine. Intl J Pharm 113:159–164

    Article  CAS  Google Scholar 

  22. Owen RW, Giacosa A, Hull WE et al (2000) Olive-oil consumption and health: the possible role of antioxidants. Lancet Oncol 1:107–112

    Article  CAS  Google Scholar 

  23. Das B, Yeger H, Baruchel H et al (2003) In vitro cytoprotective activity of squalene on a bone marrow versus neuroblastoma model of cisplatin-induced toxicity: Implications in cancer chemotherapy. Eur J Cancer 39:2556–2565

    Article  CAS  Google Scholar 

  24. Navarro-García G, Ramirez-Suarez JC, Ortega-García J, García-Camarena R et al (2009) Lipid composition, natural antioxidants and physicochemical characteristics in liver oil from rajiforms from the Gulf of Mexico. J Am Oil Chem Soc 86:323–328

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chinese Medicine Sciences and Engineering, Zhejiang University, Hangzhou, 310058, China

    Feng Wang, Xuesong Liu, Yong Chen & Longhu Wang

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  1. Feng Wang
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  2. Xuesong Liu
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  3. Yong Chen
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  4. Longhu Wang
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Correspondence to Longhu Wang.

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Wang, F., Liu, X., Chen, Y. et al. Characterization of Fatty Oil of Zizyphi spinosi semen Obtained by Supercritical Fluid Extraction. J Am Oil Chem Soc 88, 467–472 (2011). https://doi.org/10.1007/s11746-010-1700-0

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  • DOI: https://doi.org/10.1007/s11746-010-1700-0

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