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Neutral Lipid Characterization of Non-Water-Soluble Fractions of Carica Papaya Latex

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

The non-water-soluble fraction of Carica papaya latex (CPL) constitutes a waste material from papain production; very little information exists regarding its chemical composition. The non-water-soluble fraction of CPL was fractionated by liquid chromatography into neutral lipids, glycolipids and phospholipids. The most abundant compounds were found to be the polar lipids, accounting for 79.2% (w/w) of the total extractible matter, while the total amount of neutral lipids was only around 20%. It was composed of free fatty acids, sterols and triterpenic alcohols, but no glycerides were detected. A high content of saturated fatty acids was measured; these saturated fatty acids were represented by very long chains with C24:0, C26:0 and C28:0 accounting for 6.3, 11.0 and 6.3%, respectively, in the total extractible matter and 7.3, 9.0 and 3.9% in the FFA fraction. The monounsaturated fatty acids were about 23–25% in both samples, with oleic acid (C18:1) being the most abundant. The polyunsaturated fatty acids that were 25.1% in the total matter and 21.6% in the FFA fraction were mainly represented by linoleic acid (C18:2n-6). Finally, a very interesting characteristic of the FA composition of this latex concerns the presence of odd-numbered fatty acids in significant amounts (around 22% in the total extract and 24.3% in the FFA fraction).

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Acknowledgments

We are grateful to Peter Van Cauwenberghe, the Managing Director of Biohainaut, for his continuous support, and Christophe Collet (ANVAR) and Pierre Michaud (OSEO ANVAR, France) for their help in setting up this project. This study was performed in the framework of the European EUREKA LIPLANT E!3818 Project (2007–2009) with the financial support of Agence Nationale de Recherche (ANR) in France and the “Direction Générale Opérationnelle-Economie, Emploi et Recherche (DGO6)” in Belgium. We also thank Frédéric Bonfils for his help in the analysis of polyisoprene and Laurent Vaysse for our helpful discussion regarding lipids naturally found in Hevea latex. Gisèle Héral Limoux is also thanked for the nitrogen content analysis.

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Authors and Affiliations

  1. CIRAD Dept PERSYST, Ingénierie des Agro-Polymères et Technologies Emergentes, Montpellier, France

    Nathalie Barouh, Michel Pina & Pierre Villeneuve

  2. CNRS, Aix-Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse, Marseille, France

    Slim Abdelkafi & Frédéric Carrière

  3. Biohainaut, Z A E de Ghislenghien, Ghislenghien, Belgium

    Benjamin Fouquet & Frantz Scheirlinckx

Authors
  1. Nathalie Barouh
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  2. Slim Abdelkafi
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  3. Benjamin Fouquet
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  4. Michel Pina
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  5. Frantz Scheirlinckx
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  6. Frédéric Carrière
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  7. Pierre Villeneuve
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Correspondence to Pierre Villeneuve.

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Barouh, N., Abdelkafi, S., Fouquet, B. et al. Neutral Lipid Characterization of Non-Water-Soluble Fractions of Carica Papaya Latex. J Am Oil Chem Soc 87, 987–995 (2010). https://doi.org/10.1007/s11746-010-1582-1

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  • DOI: https://doi.org/10.1007/s11746-010-1582-1

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