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Carob Fruit Polyphenols Reduce Tocopherol Loss, Triacylglycerol Polymerization and Oxidation in Heated Sunflower Oil

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Journal of the American Oil Chemists' Society

Abstract

Heated oils may contain potentially toxic altered compounds. A denatured carob fiber, very rich in non-extractable tannins (Exxenterol®), exhibits antioxidant activities in in vitro experiments. The present study was designed to evaluate in sunflower oil (SO) heated to frying temperature, the protective effect on oil thermal oxidation and polymerization of adding 10 mg Exxenterol/kg oil (SO-10) and 50 mg Exxenterol/kg oil (SO-50). After 2, 8 and 16 h at 180 °C, SO displayed a relevant increase in triacylglycerol-derived polar material (PM) and polymer contents and a decrease in α-tocopherol concentrations. Thermal oxidation changes were significantly checked in SO-50 throughout the 16-h heating, while SO-10 only displayed protection from thermal oxidation during the first 2 h of heating. Oil frying-life was doubled because formation of PM and polymers was inhibited by more than 50%. Results clearly show that this non-extractable tannin-rich fiber can be successfully employed as an additive to significantly prolong sunflower oil frying-life, and thus decrease the potential toxicity of the heated oil.

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References

  1. López-Varela S, Sánchez-Muniz FJ, Cuesta C (1995) Decreased food efficiency ratio, growth retardation and changes in liver fatty acid composition in rats consuming thermally oxidized and polymerized sunflower oil used for frying. Food Chem Toxicol 33:181–189

    Article  Google Scholar 

  2. Aruna K, Rukkumani R, Sureshvarma P, Menon VP (2004) Role of an aminothiazole derivative on ethanol- and thermally oxidized sunflower oil-induced toxicity. Pol J Pharmacol 56:233–240

    CAS  Google Scholar 

  3. Romero A, Bastida S, Sánchez-Muniz FJ (2006) Cyclic fatty acid monomer formation in domestic of frozen food in sunflower oil and high oleic acid sunflower oil without oil replenishment. Food Chem Toxicol 44:1674–1681

    Article  CAS  Google Scholar 

  4. Hochgraf E, Mokady S, Cogan U (1997) Dietary oxidized linoleic acid modifies lipid composition of rat liver microsomes and increases their fluidity. J Nutr 127:681–686

    CAS  Google Scholar 

  5. Quiles JL, Huertas JR, Battino M, Ramírez-Tortosa MC, Cassinello M, Mataix J, Lopez-Frias M, Mañas M (2002) The intake of fried virgin olive or sunflower oils differentially induces oxidative stress in rat liver microsomes. Br J Nutr 88:57–65

    Article  CAS  Google Scholar 

  6. Mataix J, Barbancho FJ (2006) Olive oil in Mediterranean food. In: Quiles JL, Ramírez-Tortosa MC, Yaqoob P (eds) Olive oil and health. CAB International, Oxfordshire, pp 1–44

    Google Scholar 

  7. Sánchez-Muniz FJ, Bastida S, Márquez-Ruiz G, Dobarganes D (2007) Effect of heating and frying on oil and food fatty acids. In: Chow CK(Ed) Fatty Acids in Foods and their Health Implications. CR Press, Boca Raton, pp 511–543

    Google Scholar 

  8. Garrido-Polonio MC, Sánchez-Muniz FJ, Arroyo R, Cuesta C (1994) Small scale frying of potatoes in sunflower oil: thermoxidative alteration of the fat content in the fried product. Z Ernahrungswiss 33:267–276

    Article  CAS  Google Scholar 

  9. Dobarganes MC, Márquez-Ruiz G, Berdeaux O, Velasco J (1999) Determination of oxidation compounds and oligomers by chromatographic techniques. In: Boskou D, Elmadfa I (eds) Frying of food. oxidation, nutrient and non-nutrient antioxidants, biologically active compounds and high temperatures. Technomic Publishing Co Inc., Lancaster, pp 143–161

    Google Scholar 

  10. Sánchez-Muniz FJ (2006) Oils and fats: changes due to culinary and industrial processes. Int J Vitam Nutr Res 76:230–237

    Article  Google Scholar 

  11. Dobarganes MC, Pérez-Camino MC (1988) Fatty acid composition: a useful tool for the determination of alteration level in heated fats. Rev Fr Corps Gras 35:67–70

    CAS  Google Scholar 

  12. Varela G, Ruiz Roso B (2000) Some nutritional aspects in olive oil. In: Harwood J, Aparicio R (eds) Handbook of olive oil, analysis and properties. Aspen Publisher Inc., Gaithersburg, pp 565–580

    Google Scholar 

  13. Sánchez-Muniz FJ, Bastida S (2006) Effect of frying and thermal oxidation on olive oil and food quality. In: Quiles JL, Ramírez-Tortosa MC, Yaqoob P (eds) Olive oil and health. CAB International, Oxfordsshire, pp 74–108

    Google Scholar 

  14. Carlson BL, Tabacchi MH (1986) Frying oil deterioration and vitamin loss during food service operation. J Food Sci 51:218–221

    Article  CAS  Google Scholar 

  15. Gordon MH, Kourimska L (1995) Effect of antioxidants on losses of tocopherols during deep-fat frying. Food Chem 52:175–177

    Article  CAS  Google Scholar 

  16. Andrikopoulos NK, Dedoussis GV, Falirea A, Kalogeropoulos N, Hatzinikola HS (2002) Deterioration of natural antioxidant species of vegetable edible oils during the domestic deep-frying and pan-frying of potatoes. Inter J Food Sci Nutr 53:351–363

    Article  CAS  Google Scholar 

  17. Boskou D (1999) Non-nutrient antioxidants and stability of frying oils. In: Boskou D, Elmadfa I (eds) Frying of food. oxidation, nutrient and non-nutrient antioxidants, biologically active compounds and high temperatures. Technomic Publishing Co Inc., Lancaster, pp 183–204

    Google Scholar 

  18. Fine AM (2000) Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications. Altern Med Rev 5:144–151

    CAS  Google Scholar 

  19. Bagchi D, Krohn RL, Bagchi M, Tran MX, Stohs SJ (1997) Oxygen free radical scavenging abilities of vitamin C and E, and a grape seed proanthocyanidin extract in vitro. Res Comm Mol Pathol Pharmacol 95:179–189

    CAS  Google Scholar 

  20. Pérez-Olleros L, García-Cuevas M, Ruiz-Roso B, Requejo A (1999) Comparative study of natural carob fiber and psyllium husk in rats. Influence on some aspects of nutritional utilization and lipidaemia. J Sci Food Agric 79:173–178

    Article  Google Scholar 

  21. Pérez-Olleros L, García Cuevas M, Ruiz-Roso B (1999) Influence of pulp and natural carob fibre on some aspects of nutritional utilization and cholesterolemia in rats. Food Sci Tech Inter 5:425–430

    Article  Google Scholar 

  22. Vaquero MP, Pérez-Olleros L, García-Cuevas M, Veldhuizen M, Ruiz-Roso B, Requejo A (2000) Mineral absorption of diets containing natural carob fiber compared to cellulose, pectin and various combinations of these fibers. Food Sci Tech Inter 6:463–471

    Article  CAS  Google Scholar 

  23. Zunft HJF, Lueder W, Harde A, Haber B, Graubaum HJ, Gruenwald J (2003) Carob pulp preparation rich in insoluble fibre lowers total and LDL cholesterol in hypercholesterolemic patients. Eur J Nutr 42:235–242

    Article  CAS  Google Scholar 

  24. Ruiz-Roso B, Pérez-Olleros L, Requejo A (2003) El Exxenterol®, un extracto de fibra vegetal con un potente efecto reductor del colesterol. Schironia 2:5–9

    Google Scholar 

  25. Ruiz-Roso B, Requejo A, Haya J, Pérez-Olleros L (2008) Efectos del Exxenterol sobre los lípidos séricos de un grupo de sujetos hipercolesterolémicos. Schironia 7:21–25

    Google Scholar 

  26. Ruiz-Roso B, Pérez-Olleros L, Requejo A, Hueso JA (2007) Product of vegetal origin comprising proanthocyanidines and its preparation process. ES2245610 B1, US2008063733 A1

  27. Dobarganes MC, Velasco J, Dieffenbacher A (2000) Determination of polar compounds polymerized and oxidized triacylglycerols, and diacylglycerols in oils and fats. Results of collaborative studies and the standardized method (Technical report). Pure Appl Chem 72:1563–1575

    Article  CAS  Google Scholar 

  28. IUPAC (1992) Method 2.432. In: International union of pure and applied chemistry (ed) Standard methods for the analysis of oils, fats and derivatives. 1st supplement to the 7th edn, Pergamon Press, Oxford

  29. Sánchez-Muniz FJ, Bastida S (2003) Frying oil discarding: polar content vs. oligomer content determination. Forum Nutr 56:345–347

    Google Scholar 

  30. Lumley ID (1998) Polar compounds in heated oils. In: Varela G, Bender AE, Morton ID (eds) Frying of food, principles, changes, new approaches. Ellis Horwood, Chichester, pp 166–173

    Google Scholar 

  31. Romero A, Bastida S, Sánchez-Muniz FJ (2007) Cyclic fatty acid monomer in sunflower oils during frying of frozen foods with oil replenishment. Eur J Lipid Sci Technol 109:165–173

    Article  CAS  Google Scholar 

  32. Firestone D (1996) Regulation of frying fat and oil. In: Perkins EG, Erickson MD (eds) Deep frying. chemistry, nutrition, and practical applications. AOCS Press, Champaign, pp 323–324

    Google Scholar 

  33. DGF (German Society for Fat Research) (2000) In: Proceedings of the 3rd international symposium of deep-fat frying. Final recommendations. Eur J Lipid Sci Technol 102:594

    Google Scholar 

  34. Márquez-Ruiz G, Martín-Polvillo M, Dobarganes MC (1996) Quantitation of oxidized triglyceride monomers and dimers as a useful measurement for early and advanced stages of oxidation. Grasas y Aceites 47:48–53

    Google Scholar 

  35. Marmesat S, Velasco L, Ruiz-Méndez MV, Fernández-Martínez JM, Dobarganes C (2008) Thermostability of genetically modified sunflower oils differing in fatty acid and tocopherol compositions. Eur J Lipid Sci Technol 110:776–782

    Article  CAS  Google Scholar 

  36. Wagner KH, Elmadfa I (1999) Nutrient antioxidants and stability of frying oils: tocochromanols, β-carotene, phylloquinone, ubiquinone 50. In: Boskou D, Elmadfa I (eds) Frying of food. oxidation, nutrient and non-nutrient antioxidants, biologically active compounds and high temperatures. Technomic, Lancaster, pp 163–182

    Google Scholar 

  37. Papagiannopoulos M, Wollseifen HR, Mellenthin A, Haber B, Galensa R (2004) Identification and quantification of polyphenols in carob fruits (Ceratonia siliqua L.) and derived products by HPLC–UV–ESI/MS. J Agric Food Chem 52:3784–3791

    Article  CAS  Google Scholar 

  38. González-Muñoz MJ, Bastida S, Sánchez-Muniz FJ (1998) Short term in vivo digestibility of triacylglycerol polymers, dimmers and monomers of thermoxidized palm olein used in deep-frying. J Agric Food Chem 46:5158–5193

    Article  Google Scholar 

  39. Sánchez-Muniz FJ, Bastida S, González-Muñoz MJ (1999) Column and high performance size exclusion chromatography application to the in vivo digestibility study of a thermoxidized and polymerized olive oil. Lipids 34:1187–1192

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the Banco de Santander (Brasil) for the fellowship awarded to Daniele Zulim Botega. Financial support for this investigation was provided by the Spanish Ministerio de Educación y Ciencia Project AGL-2008-04892-C03-02, CSIC-EDOCUSA project, and Consolider-Ingenio 2010, reference CSD2007-00016.

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Authors and Affiliations

  1. Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, 28040, Madrid, Spain

    Daniele Zulim Botega, Sara Bastida, Lourdes Pérez-Olleros, Baltasar Ruiz-Roso & Francisco J. Sánchez-Muniz

  2. Instituto de la Grasa y sus Derivados, CSIC, Padre García Tejero 4, 41012, Sevilla, Spain

    Susana Marmesat

Authors
  1. Daniele Zulim Botega
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  2. Sara Bastida
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  3. Susana Marmesat
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  4. Lourdes Pérez-Olleros
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  5. Baltasar Ruiz-Roso
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  6. Francisco J. Sánchez-Muniz
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Corresponding author

Correspondence to Francisco J. Sánchez-Muniz.

Additional information

Daniele Zulim Botega is a visiting scientist from de University of Campinas, Brasil.

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Zulim Botega, D., Bastida, S., Marmesat, S. et al. Carob Fruit Polyphenols Reduce Tocopherol Loss, Triacylglycerol Polymerization and Oxidation in Heated Sunflower Oil. J Am Oil Chem Soc 86, 419–425 (2009). https://doi.org/10.1007/s11746-009-1368-5

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  • DOI: https://doi.org/10.1007/s11746-009-1368-5

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