Skip to main content
SpringerLink
Log in

Recovery of Natural Antioxidants from Olive Mill Wastewater Using Genapol-X080

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

The possibility of applying cloud point extraction (CPE) using Genapol X-080 as surfactant for the separation of phenolic compounds from olive mill wastewater was examined. The ability of the surfactant to recover individual and mixtures of polyphenols and tocopherols in various concentrations from aqueous solutions was tested before its application for the recovery of phenols from olive mill wastewater. Many of the examined individual polyphenols were recovered at high percentage. Especially, in the case of Luteolin, low surfactant concentrations were sufficient for quantitative removal. The recovery appeared proportional to the surfactant concentration. The complete recovery of tocopherols was also possible. Total phenol recovery by simple and successive CPE of olive mill wastewater with various concentrations (2, 5 and 20%, v/v) of Genapol X-080 was up to 89.5%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Antolovich M, Prenzler P, Robards K, Ryan D (2000) Sample preparation in the determination of phenolic compounds in fruits. Analyst 125:989–1009

    Article  CAS  Google Scholar 

  2. Rice-Evans AC, Miller JN, Paganda G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159

    Article  Google Scholar 

  3. Naczk M, Shahidi F (2004) Extraction and analysis of phenolics in food. J Chromatogr A 1054:95–111

    CAS  Google Scholar 

  4. Robards K (2003) Strategies for the determination of bioactive phenols in plants, fruits and vegetables. J Chromatogr A 1:2–29

    Google Scholar 

  5. Sosa Ferrera Z, Padron Sanz C, Mahugo Santana C, Santana-Rondriquez JJ (2004) The use of micellar systems in the extraction and pre-concentration of organic pollutants in environmental samples. Trends Analyt Chem 23:479–488

    Google Scholar 

  6. Mahugo Santana C, Sosa Ferrera Z, Santana Rodriguez JJ (2002) Use of non-ionic surfactant solutions for the extraction and preconcentration of phenolic compounds in water prior to their HPLC-UV detection. Analyst 127:1031–1037

    Article  CAS  Google Scholar 

  7. Katsoyannos E, Chatzilazarou A, Gortzi O, Lalas S, Konteles S, Tataridis P (2005) Application of cloud point extraction using surfactants in the isolation of physical antioxidants (phenols) from olive mill wastewater. Fresenius Environ Bull 15:1122–1125

    Google Scholar 

  8. Paleologos EK, Giokas DL, Karayannis MI (2005) Micelle-mediated separation and cloud-point extraction. Trends Analyt Chem 24:426–436

    Article  CAS  Google Scholar 

  9. Quina FH, Hinze WL (1999) Surfactant-mediated cloud point extractions: an environmentally benign alternative separation approach. Ind Eng Chem Res 38:4150–4168

    Article  CAS  Google Scholar 

  10. Carabias-Martinez R, Rondriquez-Gonzalo E, Moreno-Cordero B, Perez-Pavon JL, Garcia-Pinto C, Fernadez Laespada E (2000) Surfactant cloud point extraction and pre-concentration of organic compounds prior to chromatography and capillary electrophoresis. J Chromatogr A 902:251–265

    Article  CAS  Google Scholar 

  11. Frankewich RP, Hinze WL (1994) Evaluation and optimisation of the factors affecting non-ionic surfactant-mediated phase separations. Anal Chem 66:944–954

    Article  CAS  Google Scholar 

  12. Lindman B, Wennerstroem H (1991) Nonionic micelles grow with. increasing temperature. J Phys Chem 95:6053–6054

    Article  CAS  Google Scholar 

  13. Nilsson P, Wennerstroem H, Lindman B (1983) Structure of micellar solutions of nonionic surfactants. Nuclear magnetic resonance self-diffusion and proton relaxation studies of poly(ethylene oxide) alkyl ethers. J Phys Chem 87:1377–1385

    Article  CAS  Google Scholar 

  14. Materna K, Szymanowski J (2002) Separation of phenols from aqueous micellar solutions by cloud point extraction. J Colloid Interface Sci 255:195–201

    Article  CAS  Google Scholar 

  15. Merino F, Rubio S, Perez-Bendito D (2002) Acid-induced cloud point extraction and preconcentration of polycyclic aromatic hydrocarbons from environmental solid samples. J Chromatogr A 962:1–8

    Article  CAS  Google Scholar 

  16. Mantzavinos D, Kalogerakis N (2005) Treatment of olive mill effluents Part I. Organic matter degradation by chemical and biological processes-an overview. Environ Int 31:289–295

    Article  CAS  Google Scholar 

  17. Crognale S, D’Annibale A, Federici F, Fenice M, Quaratino D, Petruccioli M (2006) Olive oil mill wastewater valorisation by fungi. J Chem Technol Biotechnol 81:1547–1555

    Article  CAS  Google Scholar 

  18. Davies LC, Novais JM, Martins-Dias S (2004) Influence of salts and phenolic compounds on olive mill wastewater detoxification using superabsorbent polymers. Bioresource Technol 95:259–268

    Article  CAS  Google Scholar 

  19. Sirimanne SR, Patterson DG, Ma L, Justice JBJr (1998) Application of cloud-point extraction-reversed-phase HPLC. A preliminary study of the extraction and quantification of vitamins A and E in human serum and whole blood. J Chromatogr B Analyt Technol Biomed Life Sci 716:129–137

    Article  CAS  Google Scholar 

  20. Vazquez Roncero A, Graciani C, Maestro Duran R (1974) Componentes fenolicos de la aceituna I. Polifenoles de la pulpa. Grasas Aceites 25:269–279

    CAS  Google Scholar 

  21. Tsaknis J, Lalas S, Gergis V, Dourtoglou V, Spiliotis V (1999) Characterization of Moringa oleifera variety Mbololo seed oil of Kenya. J Agric Food Chem 47:4495–4499

    Article  CAS  Google Scholar 

  22. Birve SJ, Selstam E, Johansson LBAI (1996) Secondary structure of NADPH:protochlorophyllide oxidoreductase examined by circular dichroism and prediction methods. Biochem J 317:549–555

    CAS  Google Scholar 

  23. Bhairi SM (2001) Detergents: a guide to the properties and uses of detergents in biological systems. Calbiochem-Novabiochem Corporation, California

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Technology, Technological Educational Institution (T.E.I.) of Larissa (Karditsa Annex), Terma N. Temponera str, 43100, Karditsa, Greece

    Olga Gortzi & Stavros Lalas

  2. School of Food Technology and Nutrition, Technological Educational Institution (T.E.I.) of Athens, Agiou Spiridonos str, 12210, Egaleo, Athens, Greece

    Arhontoula Chatzilazarou, Evangelos Katsoyannos, Spyros Papaconstandinou & Euthalia Dourtoglou

Authors
  1. Olga Gortzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stavros Lalas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arhontoula Chatzilazarou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Evangelos Katsoyannos
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Spyros Papaconstandinou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Euthalia Dourtoglou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stavros Lalas.

About this article

Cite this article

Gortzi, O., Lalas, S., Chatzilazarou, A. et al. Recovery of Natural Antioxidants from Olive Mill Wastewater Using Genapol-X080. J Am Oil Chem Soc 85, 133–140 (2008). https://doi.org/10.1007/s11746-007-1180-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-007-1180-z

Keywords

Search

Navigation