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Assessing Glycinin (11S) and β-Conglycinin (7S) Fractions of Soybean Storage Protein by Near-Infrared Spectroscopy

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

Soybean breeding programs underway today are addressing the goal of improving the protein profile to benefit the human diet as well as that of livestock. Glycinin, a globulin storage protein of the meal and designated as the 11S size fraction by ultracentrifugation, is desirable because of its relative abundance of sulfur-containing amino acids, such as methionine and cysteine. The current study examined the feasibility of near-infrared (NIR) measurement of glycinin and the other prevalent protein fraction, β-conglycinin (7S size fraction), as well as the electrophoretically separable sub fractions that comprise these two components. From a population of 101 F6-derived recombinant inbred lines in a field replicated trial, single whole soybeans were scanned in transmittance (800–1,798 nm, 24 beans/sample × 197 samples total). Additional scanning of the ground meal was performed in reflectance (1,100–2,498 nm). Partial least squares (PLS) calibrations were developed, using the 24-bean average log(1/T) spectrum for each sample, as well as the average spectrum from duplicate packs of log(1/R) spectra of the meal. The results indicate that NIR prediction of 11S and 7S, as well as the sub fractions thereof, is at best limited to screening purposes in soybean breeding programs for probable reasons of an inherent lack of spectral specificity of the protein fractions and a non-constant proportion of soluble-to-total protein.

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Notes

  1. Trade names are provided for the purpose of description only and does not imply endorsement by the U.S. Department of Agriculture.

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Acknowledgments

The authors give thanks to B.E. Stetzler (ARS, Beltsville) for NIR reflectance and combustion protein measurements.

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Authors and Affiliations

  1. Henry A. Wallace Beltsville Agricultural Research Center, Food Safety Laboratory, USDA-ARS, Building 303, BARC-East, 10300, Baltimore Ave., Beltsville, MD, 20705-2350, USA

    Stephen R. Delwiche & Lester O. Pordesimo

  2. Department of Plant Sciences, 252 Ellington Plant Sciences, The University of Tennessee, 2431 Joe Johnson Dr., Knoxville, TN, 37996, USA

    Dilip R. Panthee & Vincent R. Pantalone

Authors
  1. Stephen R. Delwiche
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  2. Lester O. Pordesimo
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  3. Dilip R. Panthee
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  4. Vincent R. Pantalone
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Correspondence to Stephen R. Delwiche.

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Delwiche, S.R., Pordesimo, L.O., Panthee, D.R. et al. Assessing Glycinin (11S) and β-Conglycinin (7S) Fractions of Soybean Storage Protein by Near-Infrared Spectroscopy. J Am Oil Chem Soc 84, 1107–1115 (2007). https://doi.org/10.1007/s11746-007-1144-3

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  • DOI: https://doi.org/10.1007/s11746-007-1144-3

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