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Toward increasing erucic acid content in oilseed rape (Brassica napus L.) through the combination with genes for high oleic acid

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Journal of the American Oil Chemists' Society

Abstract

Erucic acid (22∶1) is a valuable renewable resource that has several applications in the oleochemical industry. High 22∶1 rapeseed (HEAR) contains around 50% 22∶1. For its technical use it is desirable to increase the 22∶1 content and to decrease the eicosenoic acid (20∶1), PUFA (18∶2+18∶3), and saturated FA (16∶0+18∶0) contents. In the present experiment, HEAR was crossed to high oleic acid rapeseed (ca. 85% 18∶1) with the hypothesis that a combination of the involved genes should lead to a reduced 18∶1 desaturation and to an increased availability of oleoyl-CoA, which should result in enhanced 22∶1 synthesis. A NIR spectroscopic calibration for 22∶1 was developed for single seeds, and the calibration was used to select, in a nondestructive manner, F2 seeds high in 22∶1. Selected F2 seeds were sown in the field and F3 seeds were harvested. The results of the FA analysis showed recombinant genotypes with increased total monounsaturated FA (22∶1+20∶1+18∶1) of up to 89% and decreased PUFA (<8%) and saturated FA content (<3.5%). There was no significant difference in 22∶1 content, but a 3 to 5% increase in 20∶1 content was observed in comparison to the HEAR parental cv. Maplus. Results were confirmed following cultivation of selected plant material a second year in the field. The present study revealed that there are other biochemical limitations than the pool of available oleoyl-CoA that restrict FA elongation to 22∶1 in rapeseed. The generated high 22∶1 plant material with an increased 18∶1 content may be useful in further studies to identify these limitations.

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Author notes

  1. Nurtjahjo Dwi Sasongko

    Present address: Faculty of Biology, The University of Jenderal Soedirman-Indonesia, Jl. dr. Soeparno-Kr-Wangkal-Purwokerto, Indonesia

Authors and Affiliations

  1. Institute for Agronomy and Plant Breeding, Georg-August University Göttingen, Von-Siebold-Str. 8, 37075, Göttingen, Germany

    Nurtjahjo Dwi Sasongko & Christian Möllers

Authors
  1. Nurtjahjo Dwi Sasongko
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  2. Christian Möllers
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Correspondence to Christian Möllers.

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Sasongko, N.D., Möllers, C. Toward increasing erucic acid content in oilseed rape (Brassica napus L.) through the combination with genes for high oleic acid. J Amer Oil Chem Soc 82, 445–449 (2005). https://doi.org/10.1007/s11746-005-1091-4

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  • DOI: https://doi.org/10.1007/s11746-005-1091-4

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