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A simple and rapid procedure for phytate determination in soybeans and soy products

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Journal of the American Oil Chemists' Society

Abstract

A rapid and simple analytical procedure to determine the phytate (myo-inositol hexaphosphate) content in soybeans and soy products is described. Minimal sample preparation and automated HPLC analysis were achieved for large numbers of samples. Determination of the phytate content in a variety of soybean samples, including animal feeds, demonstrated the simplicity, reliability, and economy of the procedure compared with other time-consuming, complicated, and laborious methods.

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References

  1. Okubo, K., G.A. Waldrop, G.A. Iacobucci, and D.V. Myers, Preparation of Low-Phytate Soybean Isolate and Concentrate by Ultracentrifugation, Cereal Chem. 52:263–271 (1975).

    CAS  Google Scholar 

  2. Hartman, G.H., Jr., Removal of Phytate from Soy Protein, J. Am. Oil Chem. Soc. 56:731–735 (1979).

    CAS  Google Scholar 

  3. Brooks, J.R., and C.V. Morr, Phytate Removal from Soy Protein Isolates Using Ion-Exchange Processing Treatments, J. Food Sci. 47:1280–1282 (1982).

    Article  CAS  Google Scholar 

  4. Brooks, J.R., and C.V. Moore, Phosphorus and Phytate Content of Soybean Protein Components, J. Agric. Food Chem. 32:674–676 (1984).

    Article  Google Scholar 

  5. Erdman, J.W., Jr., Oilseed Phytates—Nutritional Implications, J. Am. Oil Chem. Soc. 56:736–741 (1979).

    CAS  Google Scholar 

  6. Latta, M., and M. Eskin, A Simple and Rapid Colorimetric Method for Phytate Determination, J. Agric. Food Chem. 28:1313–1315 (1980).

    Article  CAS  Google Scholar 

  7. Tangendjaja, B., K.A. Buckle, and M. Wootton, Analysis of Phytic Acid by High-Performance Liquid Chromatography, J. Chromatogr. 197:274–277 (1980).

    Article  CAS  Google Scholar 

  8. Graf, E., and F.R. Dintzis, High-Performance Chromatographic Method for the Determination of Phytate, Anal. Biochem. 119:413–417 (1982).

    Article  CAS  Google Scholar 

  9. Rounds, M.A., and S.S. Nielsen, Anion-Exchange High-Performance Liquid Chromatography with Post-column Detection for the Analysis of Phytic Acid and Other Inositol Phosphates, J. Chromatogr. 653:148–152 (1993).

    Article  CAS  Google Scholar 

  10. Skoglund, E., N.-G. Carlsson, and A.-S. Sandberg, High-Performance Chromatographic Separation of Inositol Phosphate Isomers on Strong Anion Exchange Columns, J. Agric. Food Chem. 46:1877–1882 (1998).

    Article  CAS  Google Scholar 

  11. Carlsson, N.-G., E.-L. Bergman, E. Skoglund, K. Hasselblad, and A.-S. Sandberg, Rapid Analysis of Inositol Phosphates, J. Agric. Food Chem. 49:1695–1701 (2001).

    Article  CAS  Google Scholar 

  12. Wade, H.E., and D.M. Morgan, Fractionation of Phosphates by Paper Ionophoresis and Chromatography, Biochem. J. 60:264–270 (1955).

    CAS  Google Scholar 

  13. Harland, B.F., and D. Oberleas, Modified Method for Phytate Analysis Using an Ion-Exchange Procedure—Application to Textured Vegetable Proteins, Cereal Chem. 54:827–832 (1977).

    CAS  Google Scholar 

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Correspondence to Prachuab Kwanyuen.

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Kwanyuen, P., Burton, J.W. A simple and rapid procedure for phytate determination in soybeans and soy products. J Amer Oil Chem Soc 82, 81–85 (2005). https://doi.org/10.1007/s11746-005-1046-9

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  • DOI: https://doi.org/10.1007/s11746-005-1046-9

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