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Extracting long-chain fatty acids from a fermentation medium

  • Published:
Journal of the American Oil Chemists' Society

Abstract

Several solvents were evaluated for extracting free long-chain FA (LCFA) from a fermentation medium. Chloroform, chloroform/methanol (1∶1), hexane, and hexane/methyl tert-butyl ether (MTBE) (1∶1) were evaluated as alternative extraction solvents. Parameters considered for optimizing LCFA recoveries included pH and ionic strength. Maximal LCFA recoveries were obtained by adding 2 mL of the hexane/MTBE (1∶1) solvent mixture, 80 μL of 50% H2SO4, and 0.05 g NaCl to 1 mL of the aqueous sample and mixing for 15 min at 200 rpm. This method quantified saturated LCFA [capric acid (C10∶0) to stearic acid (C18∶0)] and unsaturated LCFA with 18 carbons [linoleic acid (C18∶2) and oleic acid (C18∶1)] with a 98 to 100% recovery. Caproic (C6∶0) and caprylic (C8∶0) acids were characterized by 27 and 76% recoveries, respectively.

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Authors and Affiliations

  1. Department of Civil Engineering, University of Toronto, M5S 1A4, Toronto, Ontario, Canada

    Jerald A. Lalman & David M. Bagley

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  1. Jerald A. Lalman
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  2. David M. Bagley
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Correspondence to Jerald A. Lalman.

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Lalman, J.A., Bagley, D.M. Extracting long-chain fatty acids from a fermentation medium. J Amer Oil Chem Soc 81, 105–110 (2004). https://doi.org/10.1007/s11746-004-0866-y

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  • DOI: https://doi.org/10.1007/s11746-004-0866-y

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