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Isolation and characterization of a Δ--desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4

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Journal of the American Oil Chemists' Society

Abstract

A mutant, considered to be defective in Δ9-desaturation, of an arachidonic acid-producing fungus, Mortierella alpina 1S-4, was isolated after treating the wild-type spores with N-methyl-N′-nitro-N-nitrosoguanidine. The mycelial FA of this mutant included 38% stearic acid, the level being only 5% for the wild type. Upon growth at 24–28°C, its mycelial lipids included a high level (up to 50 mol% of total mycelial lipids) of FFA, of which about 90 mol% was stearic acid. However, the level of FFA was markedly decreased with a concomitant increase in the IAG level when the mutant was grown at 20°C or lower, or when it was grown in a culture medium supplemented with PUFA.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, 606-8502, Kyoto, Japan

    Saeree Jareonkitmongkol, Eiji Sakuradani & Sakayu Shimizu

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  1. Saeree Jareonkitmongkol
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  2. Eiji Sakuradani
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  3. Sakayu Shimizu
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Correspondence to Sakayu Shimizu.

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Jareonkitmongkol, S., Sakuradani, E. & Shimizu, S. Isolation and characterization of a Δ--desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J Amer Oil Chem Soc 79, 1021–1026 (2002). https://doi.org/10.1007/s11746-002-0596-1

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  • DOI: https://doi.org/10.1007/s11746-002-0596-1

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