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Distribution of α-and γ-tocopherols in Atlantic salmon (Salmo salar) tissues

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Lipids

Abstract

Groups of Atlantic salmon parr (mean initial weight 9.5 g) were fed three diets, the first containing no tocopherol supplement, the others supplemented with either all-rac-α-tocopherol (A-T) or RRR-γ-tocopherol (G-T). Tocopherol concentrations in the liver, serum, testes, kidney, brain, gill, muscle, and perivisceral fat were measured after 36 wk. Despite a higher dietary intake of G-T, compared to A-T, deposition of γ-tocopherol (γT) was less efficient than of α-tocopherol (αT) in most tissues except in the perivisceral fat, an adipose tissue. In fish fed the G-T diet, the γT/αT ratio was highest in the perivisceral fat and lowest in the liver, indicating that the liver is the most discriminatory organ for retaining αT as compared to γT, and the perivisceral fat is more suitable for the storage of γT. A negative correlation (P<0.01) was observed between the γT/αT ratio and the corresponding tissue phospholipid content, suggesting that γT is less efficiently deposited compared to αT in the phospholipid-rich membranes which are presumed to be the functional site for lipid antioxidants in vivo. During restricted intake of αT, the liver and muscle exhibited the greatest reduction of this tocopherol among the tissues analyzed. The presence of minimal αT in the muscle from fish fed the tocopherol-unsupplemented diet led to greater susceptibility to lipid peroxidation after frozen storage than was the case for muscle containing higher concentrations of either αT or γT. However, both αT and γT were effective stabilizers of salmon muscle lipids during frozen storage.

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Abbreviations

A-T:

all-rac-α-tocopherol-supplemented diet

G-T:

RRR-γ-tocopherol-supplemented diet

5-NS:

5-doxylstearate

16-NS:

16-doxylstearate

TBARS:

2-thiobarbituric acid-reactive substances

no-T:

nontocopherol-supplemented diet

αT:

α-tocopherol

γT:

γ-tocopherol

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Author information

Author notes

  1. Santosh P. Lall

    Present address: Institute for Marine Biosciences, National Research Council, 1411 Oxford St., B3H 3Z1, Halifax, Nova Scotia, Canada

  2. John D. Castell

    Present address: Department of Fisheries and Oceans, Brandy Cove Rd., E0G 2X0, St. Andrews, New Brunswick, Canada

Authors and Affiliations

  1. Department of Fisheries and Oceans, B3J 2S7, Halifax, Nova Scotia, Canada

    Santosh P. Lall & John D. Castell

  2. Canadian Institute of Fisheries Technology, DalTech, Dalhousie University, P.O. Box 1000, B3J 2X4, Halifax, Nova Scotia, Canada

    Monina P. M. Parazo & Robert G. Ackman

Authors
  1. Monina P. M. Parazo
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  2. Santosh P. Lall
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  3. John D. Castell
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  4. Robert G. Ackman
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Corresponding author

Correspondence to Robert G. Ackman.

Additional information

Presented in part at the Annual Meeting of the American Oil Chemists' Society, San Antonio, Texas, May 1995.

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Parazo, M.P.M., Lall, S.P., Castell, J.D. et al. Distribution of α-and γ-tocopherols in Atlantic salmon (Salmo salar) tissues. Lipids 33, 697–704 (1998). https://doi.org/10.1007/s11745-998-0259-x

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  • DOI: https://doi.org/10.1007/s11745-998-0259-x

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