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Dietary docosahexaenoic acid and immunocompetence in young healthy men

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Lipids

Abstract

The purpose of this study was to examine the effect of dietary docosahexaenoic acid (DHA), in the absence of eicosapentaenoic acid, on human immune response (IR). A 120-d study with 11 healthy men was conducted at the Metabolic Research Unit of the Western Human Nutrition Research Center. Four subjects (control group) were fed the stabilization or basal diet (15, 30, and 55% energy from protein, fat, and carbohydrate, respectively) throughout the study; the remaining seven subjects (DHA group) were fed the basal diet for the first 30 d, followed by 6 g DHA/d for the next 90 d. DHA replaced an equivalent amount of linoleic acid; the two diets were comparable in their total fat and all other nutrients. Both diets were supplemented with 20 mg d-α-tocopherol acetate per day. Indices of IR were examined on study day 22, 30, 78, 85, 106, and 113. Addition of DHA at moderately high levels did not alter the proliferation of peripheral blood mononuclear cells cultured with phytohemag-glutinin or concanavalin A, or the delayed hypersensitivity skin response. Also, additional DHA did not alter the number of T cells producing interleukin 2 (IL2), the ratio between the helper/suppressor T cells in circulation, or the serum concentrations of immunoglobulin G, C3, and interleukin 2 receptor (IL2R). DHA supplementation, however, caused a significant (P=0.0001) decrease in the number of circulating white blood cells which was mainly due to a decrease in the number of circulating granulocytes. The number of lymphocytes in peripheral circulation was not affected by Dietary DHA enrichment, but the percentage of lymphocytes in white blood cells increased because of a reduction in granulocyte numbers. None of these indices was changed in the control group. Our results show that when total fat intake is low and held constant, DHA consumption does not inhibit many of the lymphocyte functions which have been reported to be inhibited by fish oil consumption.

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Abbreviations

Bq:

Bacquerel

CD:

cluster designation

Con A:

concanavalin A

CTL:

cytotoxic T

DHA:

docosahexaenoic acid

DTH:

delayed type hypersensitivity skin response

EPA:

eicosapentaenoic acid

IL:

interleukin

IR:

immune response

LA:

linoleic acid

LTB4 :

leukotriene B4

PBMNC:

peripheral blood mononuclear cells

PHA:

phytohemagglutinin

PMN:

polymorphonuclear leukocytes

PUFA:

polyunsaturated fatty acid

WBC:

white blood cell

References

  1. Kelley, D.S., Branch, L.B., and Iacono, J.M. (1989) Nutritional Modulation of Human Immune Status, Nutr. Res. 9, 965–975.

    Article  CAS  Google Scholar 

  2. Kelley, D.S., Dougherty, R.M., Branch, L.B., Taylor, P.C., and Iacono, J.M. (1992) Concentration of Dietary n-6 Polyunsaturated Fatty Acids and Human Immune Status, Clin. Immunol. Immunopathol. 62, 240–244.

    Article  PubMed  CAS  Google Scholar 

  3. Meydani, S.N., Lichtenstein, A.H., Cornwall, S., Meydani, M., Goldin, B.R., Rasmussen, H., Dinarello, C.A., and Schaefer, E.J. (1993) Immunologic Effects of National Cholesterol Education Panel Step-2 Diets with and without Fish-Derived n-3 Fatty Acid Enrichment, J. Clin. Invest. 92, 105–113.

    PubMed  CAS  Google Scholar 

  4. Barone, J., Hebert, J.R., and Reddy, M.M. (1989) Dietary Fat and Natural Killer Cell Activity, Am. J. Clin. Nutr. 50, 861–867.

    PubMed  CAS  Google Scholar 

  5. Kelley, D.S., Taylor, P.C., Nelson, G.J., Schmidt, P.C., Mackey, B.E., and Kyle, D. (1997) Effects of Dietary Arachidonic Acid on Human Immune Response, Lipids 32, 449–456.

    PubMed  CAS  Google Scholar 

  6. Kelley, D.S., Branch, L.B., Love, J.E., Taylor, P.C., Rivera, Y.M., and Iacono, J.M. (1991) Dietary α-Linolenic Acid and Immunocompetence in Humans, Am. J. Clin. Nutr. 53, 40–46.

    PubMed  CAS  Google Scholar 

  7. Hughes, D.A., Pinder, A.C., Piper, Z., Johnson, I.T., and Lund, E.K. (1996) Fish Oil Supplementation Inhibits the Expression of Major Histocompatibility Complex Class II Molecules and Adhesion Molecules on Human Monocytes, Am. J. Clin. Nutr. 63, 267–272.

    PubMed  CAS  Google Scholar 

  8. Bjerve, K.S., Fischer, S., Wammer, F., and England, T. (1989) α-Linolenic Acid and Long-chain ω-3 Fatty Acid Supplementation in Three Patients with ω-3 Fatty Acid Deficiency, Am. J. Clin. Nutr. 49, 290–300.

    PubMed  CAS  Google Scholar 

  9. Caughey, G.E., Montzioris, E., Gibson, R.A., Cleland, L.G., and James, M.J. (1996) The Effect on Human Tumor Necrosis Factor α and Interleukin 1β Production of Diets Enriched in n-3 Fatty Acids from Vegetable Oil or from Fish Oil, Am. J. Clin. Nutr. 63, 116–122.

    PubMed  CAS  Google Scholar 

  10. Endres, S., Ghorbani, R., Kelley, V.E., Georgilis, K., Lonnemann, G., Van der Meer, J.W.M., Cannon, J.G., Rogers, T.S., Klempner, M.S., Weber, P.C., Schaefer, E.J., Wolff, S.M., and Dinarello, C.A. (1989) The Effect of Dietary Supplementation with n-3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells, N. Eng. J. Med. 320, 265–271.

    Article  CAS  Google Scholar 

  11. Endres, S., Meydeni, S.N., Ghorbani, R., Schindler, R., and Dinarello, C.A. (1993) Dietary Supplementation with n-3 Fatty Acids Suppresses Interleukin-2 Production and Mononuclear Cell Proliferation, J. Leukocyte Biolog. 54, 599–603.

    CAS  Google Scholar 

  12. Lee, T.H., Hoover, R.L., Williams, J.D., Sperling, R.I., Ravalese, J., Spur, B.W., Robinson, D.R., Corey, E.J., Lewis, R.A., and Austen, K.F. (1985) Effect of Dietary Enrichment with Eicosapentaenoic acid and Docosahexaenoic Acids on in vitro Neutrophil and Monocyte Leukotriene Generation and Neutrophil Function, N. Engl. J. Med. 312, 1217–1224.

    Article  PubMed  CAS  Google Scholar 

  13. Payan, D.G., Wong, M.Y.S., Chernov-Rogan, T., Valone, F.H., Pickett, W.C., Blake, V., and Gold, W.M. (1986) Alteration in Human Leukocyte Function Induced by Ingestion of Eicosapentaenoic Acid, J. Clin. Immunol. 6:402–410.

    Article  PubMed  CAS  Google Scholar 

  14. Virella, G., Kilpatrick, J.M., Rugeles, M.R., Hayman, B., and Russell, R. (1989) Depression of Humoral Responses and Phagocytic Functions in vivo and in vitro by Fish Oil and Eicosapentaenoic Acid, Clin. Immunol. Immunopathol. 52, 257–270.

    Article  PubMed  CAS  Google Scholar 

  15. Kramer, T.R., Schoene, N., Douglass, L.W., Judd, J.W., Ballard-Barbash, J.T., Taylor, R., Bhagavan, P.R., and Nair, P.P. (1991) Increased Vitamin E Intake Restores Fish Oil-induced Suppressed Blastogenesis of Mitogen-Stimulated T Lymphocytes, Am. J. Clin. Nutr. 54, 896–902.

    PubMed  CAS  Google Scholar 

  16. Molvig, J., Pociot, F., Worssaae, H., Wogensen, L.D., Back, L., Christensen, P., Mandrup-Poulsen, T., Anderson, K., Madsen, P., Dyerberg, J., and Nerup, J. (1991) Dietary Supplementation with ω-3-Polyunsaturated Fatty Acids Decreases Mononuclear Cell Proliferation and Interleukin-1β Content But Not Monokine Secretion in Healthy and Insulin-Dependent Diabetic Individuals, Scand. J. Immunol. 34, 399–410.

    Article  PubMed  CAS  Google Scholar 

  17. Soyland, E., Lea, T., Sandstad, B., and Drevon, A. (1994) Dietary Supplementation with Very Long-Chain n-3 Fatty Acids in Man Decreases Expression of the Interleukin-2 Receptor (CD25) on Mitogen-Stimulated Lymphocytes from Patients with Inflammatory Skin Diseases, Eur. J. Clin. Invest. 24, 236–242.

    Article  PubMed  CAS  Google Scholar 

  18. Wallace, J.M.W., Turley, E., Gilmore, W.S., and Strain, J.J. (1995) Dietary Fish Oil Supplementation Alters Leukocyte Function and Cytokine Production in Healthy Women, Arterioscler. Thromb. Vasc. Biol. 15, 185–189.

    PubMed  CAS  Google Scholar 

  19. Yamashita, N., Maruyama, M., Yamazaki, K., Hamazaki, T., and Yano, S. (1991) Effect of Eicosapentaenoic Acid and Docosahexaenoic Acid on Natural Killer Cell Activity in Human Peripheral Blood Lymphocytes, Clin. Immunol. and Immunopathol. 59, 335–345.

    Article  CAS  Google Scholar 

  20. Kremer, J.M., Lawrence, D.A., Petrillo, G.F., Litts, L.L., Mullaly, P.M., Rynes, R.I., Stocker, R.P., Parhami, N., Greenstein, N.S., Fuchs, B.R., Mathur, A., Robinson, D.R., Sperling, R.I., and Bigaouette, J. (1995) Effects of High-dose Fish Oil on Rheumatoid Arthritis After Stopping Nonsteroidal Antiinflammatory Drugs, Arthritis Rheum. 38, 1107–1114.

    PubMed  CAS  Google Scholar 

  21. Walton, A.J.E., Snaith, M.L., Locniskar, M., Cumberland, A.G., Morrow, W.J.W., and Isenberg, D.A. (1991) Dietary Fish Oil and the Severity of Symptoms in Patients with Systemic Erythematosus, Ann. Rheum. Dis. 50, 463–466.

    PubMed  CAS  Google Scholar 

  22. Kojima, T., Terano, T., Tanabe, E., Okamoto, S., Tamura, Y., and Yoshida, S. (1989) Effect of Highly Purified Eicosapentaenoic Acid on Psoriasis, J. Am. Acad. Dermatol. 21, 150–151.

    Article  PubMed  CAS  Google Scholar 

  23. Lawrence, R., and Sorrell, T. (1993) Eicosapentaenoic Acid in Cystic Fibrosis, Lancet 342, 465–469.

    Article  PubMed  CAS  Google Scholar 

  24. Stenson, W.F., Cort, D., Rogers, J., Burakoff, R., Deschyver-Kecskemeti, K., Gramlich, T.L., and Beeken, W. (1992) Dietary Supplementation with Fish Oil in Ulcerative Colitis, Ann. Intern. Med. 116, 609–614.

    PubMed  CAS  Google Scholar 

  25. Khalfoun, B., Thibault, G., Lacord, M., Bardos, P., and Lebranchu, Y. (1996) Docosahexaenoic and Eicosapentaenoic Acids Inhibit Human Lymphoproliferative Responses in vitro but Not the Expression of T Cell Surface Markers, Scand. J. Immunol. 43, 248–256.

    PubMed  CAS  Google Scholar 

  26. Lim, B.O., Yamada, K., Hung, P., Watanabe, T., Taniguchi, S., and Sugano, M. (1996) Effects of n-3 Polyunsaturated Fatty Acids and Lectins on Immunoglobulin Production by Spleen Lymphocytes of Sprague-Dawley Rats, Biosci. Biotech. Biochem. 60, 1025–1027.

    Article  CAS  Google Scholar 

  27. Jolly, C., Jiang, Y.H., Chapkin, R.S., and McMurray, D. (1997) Dietary n-3 Polyunsaturated Fatty Acids Suppress Murine Lymphoproliferation, Interleukin 2 Secretion, and the Formation of Diacylglycerol and Ceramide, J. Nutr. 127, 37–43.

    PubMed  CAS  Google Scholar 

  28. U.S. Department of Agriculture, (1987) Composition of Foods, in Revised Agriculture Handbook no. 8.1-8.14, Washington D.C., U.S. Government Printing Office.

    Google Scholar 

  29. Littell, R.C., Milliken, G.A., Stroup, W.W., and Wolfinger, R.D. (1996) SAS TM System for Mixed Models, p. 633, SAS Institute Inc., Cary.

    Google Scholar 

  30. Brossard, N., Crosset, M., Pachiudi, C., Riou, J.P., and Tayot, J.L. (1996) Retroconversion and Metabolism of 13C, 22∶6n-3 in Humans and Rats After Intake of a Single Dose of 13C, 22∶6n-3-Triacylglycerols, Am. J. Clin. Nutr. 64, 577–586.

    PubMed  CAS  Google Scholar 

  31. Conquer, J.A., and Holub, B.J. (1997) Dietary Docosahexaenoic Acid as a Source of Eicosapentaenoic Acid in Vegetarians and Omnivores, Lipids 32, 341–345.

    PubMed  CAS  Google Scholar 

  32. Nelson, G.J., Schmidt, P.C., Bartolini, G.L., Kelley, D.S., and Kyle, D. (1997) The Effect of Dietary Docosahexaenoic Acid on Plasma Lipoproteins and Tissue Fatty Acid Composition in Humans, Lipids 32, 1137–1146.

    PubMed  CAS  Google Scholar 

  33. Knapp, H.R., and Fitzgerald, G.A. (1986) Effects of Dietary Fishoil on Human Leukocyte Function, Clin. Res. 34, 390A.

    Google Scholar 

  34. Gabor, H., and Abraham, S. (1986) Effect of Dietary Menhaden Oil on Tumor Cell Loss and the Accumulation of a Mass Transplantable Mammary Adenocarcinoma in BALB/c Mice, J. Natl. Cancer Inst. 76, 1223–1229.

    PubMed  CAS  Google Scholar 

  35. Rose, D.P., and Connoly, J.M. (1993) Effect of Dietary Omega-3 Fatty Acids on Human Breast Cancer Growth and Metastases in Nude Mice, J. Natl. Cancer Inst. 85:1743–1747.

    PubMed  CAS  Google Scholar 

  36. Atkinson, T.G., Barker, H.J., and Meckling-Gill, K.A. (1997) Incorporation of Long-Chain n-3 Fatty Acids in Tissues, and Enhanced Bone Marrow Cellularity with Docosahexaenoic Acid Feeding in Post-Weanling Fischer 344 Rats, Lipids 32, 293–302.

    PubMed  CAS  Google Scholar 

  37. Doukas, J., Hechman, H.B., and Shepro, D. (1988) Endothelial Secreted Arachidonic Acid Metabolites Modulate Polymorphonuclear Leukocyte Chemotaxis, Blood 71, 771–779.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Western Regional Research Center, Presidio of San Francisco, California

    P. C. Taylor, G. J. Nelson & B. E. Mackey

  2. Western Human Nutrition Research Center, USDA/ARS, P.O. Box 29997, 94129, Presidio of San Francisco, CA

    D. S. Kelley, P. C. Taylor, G. J. Nelson & B. E. Mackey

Authors
  1. D. S. Kelley
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  2. P. C. Taylor
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  3. G. J. Nelson
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  4. B. E. Mackey
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Correspondence to D. S. Kelley.

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Kelley, D.S., Taylor, P.C., Nelson, G.J. et al. Dietary docosahexaenoic acid and immunocompetence in young healthy men. Lipids 33, 559–566 (1998). https://doi.org/10.1007/s11745-998-0240-8

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  • DOI: https://doi.org/10.1007/s11745-998-0240-8

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