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Effect of n−3 fatty acid supplementation on lipid peroxidation and protein aggregation in rat erythrocyte membranes

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Lipids

Abstract

Human erythrocytes in the circulation undergo dynamic oxidative damage involving membrane lipid peroxidation and protein aggregation during aging. The present study was undertaken to determine the effect of n−3 fatty acid supplementation on lipid peroxidation and protein aggregation in the circulation and also the in vitro susceptibility of rat erythrocyte membranes to oxidative damage. Wistar male rats were fed a diet containing n−6 fatty acid-rich safflower oil or n−3 fatty acid-rich fish oil with an equal amount of vitamin E for 6 wk. n−3 Fatty acid content in erythrocyte membranes of rats fed fish oil was significantly higher than that of rats fed safflower oil. The degree of membrane lipid peroxidation and protein aggregation of rats fed fish oil was not significantly higher than that of rats fed safflower oil when the amounts of phospholipid hydroper-oxides, thiobarbituric acid-reactive substances, and detergent-insoluble protein aggregates were measured. When isolated erythrocytes were oxidized under aerobic conditions in the presence of Fe(III), the degree of membrane lipid peroxidation of erythrocytes from rats fed fish oil was increased to a greater extent than that of rats fed safflower oil, whereas the degree of membrane protein aggregation of both groups was increased in a similar extent. Hence, n−3 fatty acid supplementation did not affect lipid peroxidation and protein aggregation in membranes of circulating rat erythrocytes, and the supplementation increased the susceptibility of isolated erythrocytes to lipid peroxidation, but not to protein aggregation, under the aerobic conditions. If a sufficient amount of vitamin E is supplied, n−3 fatty acid supplementation may give no undesirable oxidative effects on rat erythrocytes in the circulation.

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Abbreviations

BHT:

butylated hydroxytoluene

C12E8 :

octaethylene glycol n-dodecyl monoether

DPBS:

Dulbecco's phosphate-buffered saline

DPBS(−):

Ca2+- and Mg2+-free DPBS

HPLC:

high-performance liquid chromatography

PCOOH:

phosphatidylcholine hydroperoxide

PEOOH:

phosphatidylethanolamine hydroperoxide

SDS-PAGE:

SDS-polyacrylamide gel electrophoresis

TBA:

thiobarbituric acid

TBARS:

TBA-reactive substance

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Author information

Authors and Affiliations

  1. Kagawa Nutrition University, Sakado, 350-02, Saitama, Japan

    Terue Kawabata & Kyoko Hasegawa

  2. Oleochemical Research Laboratory, Amagasaki, 660, Osaka, Japan

    Masao Suzuki

  3. School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan

    Ken Ando, Kunihide Nagata, Masatoshi Beppu & Kiyomi Kikugawa

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  1. Ken Ando
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  2. Kunihide Nagata
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  3. Masatoshi Beppu
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  4. Kiyomi Kikugawa
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  5. Terue Kawabata
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  6. Kyoko Hasegawa
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  7. Masao Suzuki
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Correspondence to Kiyomi Kikugawa.

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Ando, K., Nagata, K., Beppu, M. et al. Effect of n−3 fatty acid supplementation on lipid peroxidation and protein aggregation in rat erythrocyte membranes. Lipids 33, 505–512 (1998). https://doi.org/10.1007/s11745-998-0234-6

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  • DOI: https://doi.org/10.1007/s11745-998-0234-6

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