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Lipid molecular order in liver mitochondrial outer membranes, and sensitivity of carnitine palmitoyltransferase I to malonyl-CoA

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Lipids

Abstract

Mitochondrial outer membranes were prepared from livers of rats that were in the normal fed state, starved for 48 h, or made diabetic by injection of streptozotocin. Membranes were also prepared from starved late-pregnant rats. The latter three conditions have previously been shown to induce varying degrees of desensitization of mitochondrial overt carnitine palmitoyltransferase (CPT I) to malonyl-CoA inhibition. We measured the fluorescence polarization anisotropy of two probes, 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene-p-toluenesulfonate (TMA-DPH) which, when incorporated into membranes, report on the hydrophobic core and on the peripheral regions of the bilayer, respectively. The corresponding polarization indices (r DPH and r TMA-DPH) were calculated. In membranes of all three conditions characterized by CPT I desensitization to malonyl-CoA, r DPH was decreased, whereas there was no change in r TMA-DPH, indicating that CPT I is sensitive to changes in membrane core, rather than peripheral, lipid order. The major lipid components of the membranes were analyzed. Although significant changes with physiological state were observed, there was no consistent pattern of changes in gross lipid composition accompanying the changes to membrane fluidity and CPT I sensitivity to malonyl-CoA. We conclude that CPT I kinetic characteristics are sensitive to changes in lipid composition that are localized to specific membrane microdomains.

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Abbreviations

CPT I:

the outer-membrane carnitine palmitoyltransferase of mitochondria

DPH:

1,6-diphenyl-1,3,5-hexatriene

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

TMA-DPH:

1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene-p-toluene sulfonate

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  1. Hannah Research Institute, KA6 5HL, Ayr, Scotland, UK

    Victor A. Zammit, Clark G. Corstorphine, Marys P. Kolodziej & Fiona Fraser

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  1. Victor A. Zammit
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  2. Clark G. Corstorphine
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  3. Marys P. Kolodziej
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  4. Fiona Fraser
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Correspondence to Victor A. Zammit.

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Zammit, V.A., Corstorphine, C.G., Kolodziej, M.P. et al. Lipid molecular order in liver mitochondrial outer membranes, and sensitivity of carnitine palmitoyltransferase I to malonyl-CoA. Lipids 33, 371–376 (1998). https://doi.org/10.1007/s11745-998-0217-7

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  • DOI: https://doi.org/10.1007/s11745-998-0217-7

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