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Failure of the nonselective β-blocker propranolol to affect lipoprotein lipase gene expression in the rat

  • Published:
Lipids

Abstract

Treatment with β-blockers has been reported to be associated with the development of hypertriglyceridemia. The etiology, even the existence, of this phenomenon is controlverisal. The purpose of our study was to examine whether the nonselective β-blocker propranolol causes hypertriglyceridemia in the rat and whether its action is mediated by the modulation of lipoprotein lipase (LPL) messenger RNA (mRNA) accumulation or activity. LPL activity was assayed in fresh tissue by incubation with tritiated triglycerides. LPL mRNA was quantified in total RNA by slot-blot analysis using a mouse LPL complementary DNA probe. We have conducted three series of experiments in unanaesthetized rats in order to study the effects of different single doses of propranolol (1.5 to 6 mg i.p.) and different durations of treatment (15 min to 4 wk). We measured triglyceride and cholesterol levels in plasma as well as the LPL activity and mRNA levels in the heart and adipose tissue before and after propranolol administration. In these experiments we did not find any significant decrease in either the activity or the amount of mRNA of lipoprotein lipase nor was there any change in plasma lipids following treatment. Our results lead us to the conclusion that the nonselective β-blocker propranolol affects neither the activity nor, the mRNA level of LPL in the rat.

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Abbreviations

cRNA:

complementary RNA

LPL:

lipoprotein lipase

mRNA:

messenger RNA

TG:

triglycerides

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Author notes

  1. I. Gouni-Berthold

    Present address: Department of Internal Medicine, Medizinische Poliklinik, University of Bonn, Sigmund-Freud-Strasse 25, 53111, Bonn, Germany

Authors and Affiliations

  1. Departments of Medicine and Cell Biology, Baylor College of Medicine, 77030, Houston, Texas

    I. Gouni-Berthold, K. Oka & L. Chan

  2. Department of Clinical Pharmacology, University of Bonn, 53105, Bonn, Germany

    H. K. Berthold

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  1. I. Gouni-Berthold
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  2. K. Oka
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  4. L. Chan
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Correspondence to H. K. Berthold.

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Gouni-Berthold, I., Oka, K., Berthold, H.K. et al. Failure of the nonselective β-blocker propranolol to affect lipoprotein lipase gene expression in the rat. Lipids 32, 943–947 (1997). https://doi.org/10.1007/s11745-997-0121-1

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  • DOI: https://doi.org/10.1007/s11745-997-0121-1

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