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The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation

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Lipids

Abstract

The aim of this study was to measure the changes in lipid metabolism which occur during smoltification and seawater transfer in Atlantic salmon (Salmo salar). Duplicate groups of Atlantic salmon parr were fed diets containing either fish oil (FO) or a blend of linseed and rapeseed oils, vegetable oil (VO), from October (week 0) to seawater transfer in May (week 26). From May to August (weeks 26–43), all fish were fed a fish oil-containing diet. Fatty acyl desaturation and elongation activity were followed in isolated hepatocytes incubated with radioactive 18:3n−3 and 18:2n−6. Metabolism of 18:3n−3 was consistently around 5-fold greater than metabolism of 18:2n−6, and total metabolism of both substrate polyunsaturated fatty acids (PUFA) was increased in fish fed both VO and FO up to seawater transfer after which desaturation activities were reduced. Desaturation activities with both 18:3n−3 and 18:2n−6 were significantly greater in fish fed VO, compared to fish fed FO, at 22 and 26 wk. Arachidonic acid (20:4n−6; AA) in liver polar lipids (PL) of fish fed VO increased consistently from weeks 0–22 but varied after seawater transfer. In fish fed FO, AA in liver PL remained constant up to week 17 before increasing at seawater transfer and leveling off thereafter. Eicosapentaenoic acid (20:5n−3; EPA) in liver PL of fish fed VO decreased significantly from week 0–22 before rising at seawater transfer and increasing rapidly posttransfer. EPA in liver PL of fish fed FO showed a similar trend except EPA was always greater in the freshwater phase compared to fish fed VO. Docosahexaenoic acid (DHA) levels in liver PL of fish fed VO remained constant in the freshwater phase before increasing following seawater transfer. In fish fed FO, DHA in liver PL increased from weeks 0–17 reducing and leveling off postseawater transfer. The levels of PGF2α and PGF3α were measured in isolated gill cells stimulated with calcium ionophore A23187. PGF2α production in fish fed VO increased significantly between 0–7 wk before decreasing toward seawater transfer. After transfer, PGF2α production increased to a peak at 35 wk. PGF2α production in fish fed FO was not significantly altered during the trial period. The changes in PGF3α production were broadly similar to those occurring with PGF2α, but the latter was always in excess of the former (2-to 4-fold). Plasma chloride concentrations in fish subjected to seawater challenge at 20 wk were significantly lower in fish fed VO compared to those fed FO. This study has provided new information on the changes in lipid metabolism which accompany parr-smolt transformation and suggests that diets which have a fatty acid composition more similar to that in aquatic invertebrates may be beneficial in effecting successful seawater adaptation.

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Abbreviations

AA:

arachidonic acid

BHT:

butylated hydroxytoluene

DHA:

docosahexaenoic acid

EFA:

essential fatty acid

EPA:

eicosapentaenoic acid

FAF-BSA:

fatty acid free bovine serum albumin

FO:

fish oil

HBSS:

Hank’s balanced salt solution

MHM:

Marine Harvest McConnell Ltd.

PGF:

prosta-glandin F

PL:

polar lipid

PUFA:

polyunsaturated fatty acid

TLC:

thin-layer chromatography

VO:

vegetable oil

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Authors and Affiliations

  1. N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Sciences, University of Stirling, FK9 4LA, Stirling, Scotland

    J. Gordon Bell, Douglas R. Tocher, Bruce M. Farndale & John R. Sargent

  2. Blar Mhor Industrial Estate, Fort, Marine Harvest McConnell Ltd., PH33 7PT, Highland, William, Scotland

    David I. Cox

  3. Fish Feed Group, BOCM-Pauls, Renfrew Mill, PA4 8AH, Strathclyde, Renfrew, Renfrewshire, Scotland

    Richard W. McKinney

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  1. J. Gordon Bell
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Bell, J.G., Tocher, D.R., Farndale, B.M. et al. The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation. Lipids 32, 515–525 (1997). https://doi.org/10.1007/s11745-997-0066-4

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  • DOI: https://doi.org/10.1007/s11745-997-0066-4

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