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Effects of dietary arachidonic acid on human immune response

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Lipids

Abstract

Arachidonic acid (AA) is a precursor of eicosanoids, which influence human health and the in vitro activity of immune cells. We therefore examined the effects of dietary AA on the immune response (IR) of 10 healthy men living at our metabolic suite for 130 d. All subjects were fed a basal diet containing 27 energy percentage (en%) fat, 57 en% carbohydrate, and 16 en% protein (AA, 200 mg/d) for the first and last 15 d of the study. Additional AA (1.5 g/d) was incorporated into the diet of six men from day 16 to 65 while the remaining four subjects continued to eat the basal diet. The diets of the two groups were crossed-over from day 66 to 115. In vitro indexes of IR were examined using the blood samples drawn on days 15, 58, 65, 108, 115, and 127. The subjects were immunized with the measles/mumps/rubella vaccine on day 35 and with the influenza vaccine on day 92. Dietary AA did not influence many indexes of IR (peripheral blood mononuclear cell proliferation in response to phytohemagglutinin, Concanavalin A, pokeweed, measles/mumps/rubella, and influenza vaccines prior to immunization, and natural killer cell activity). The postimmunization proliferation in response to influenza vaccine was about fourfold higher in the group receiving high-AA diet compared to the group receiving low-AA diet (P=0.02). Analysis of variance of the data pooled from both groups showed that the number of circulating granulocytes was significantly (P=0.03) more when the subjects were fed the high-AA diet than when they were fed the low-AA diet. The small increases in granulocyte count and the in vitro proliferation in response to influenza vaccine caused by dietary AA may not be of clinical significance. However, the lack of any adverse effects on IR indicates that supplementation with AA may be done safely when needed for other health reasons.

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Abbreviations

AA:

arachidonic acid

CD:

cluster designation

Con A:

concanavalin A

DTH:

delayed-type hypersensitivity skin response

en%:

energy percentage

FBS:

fetal bovine serum

II-2:

interleukin-2

INF:

interferon

LA:

linoleic acid

MMR:

measles/mumps/rubella

MP:

metabolic period

NK:

natural killer

PGE:

prostaglandin E

LTB4 :

leukotriene B4

PBMNC:

peripheral blood mononuclear cells

PUFA:

polyunsaturated fatty acids

PHA:

phytohemagglutinin

RDA:

recommended daily allowance

References

  1. Kelley, D.S., Branch, L.B., and Iacono, J.M. (1989) Nutritional Modulation of Human Immune System, Nutr. Res. 9:965–975.

    Article  CAS  Google Scholar 

  2. Kelley, D.S., Dougherty, R.M., Branch, L.B., Taylor, P.C. and Iacono, J.M. (1992) Concentration of Dietary n-6 Polyunsaturated Fatty Acids and the Human Immune Status, Clin. Immun. Immunopath. 62:240–244.

    Article  PubMed  CAS  Google Scholar 

  3. Barone, J., Hebert, J.R., and Reddy, M.M. (1989) Dietary Fat and Natural Killer Cell Activity, Am. J. Clin. Nutr. 50, 861–867.

    PubMed  CAS  Google Scholar 

  4. Kelley, D.S., Branch, L.B., Love, J.E., Taylor, P.C., Rivera, Y.M., and Iacono, J.M. (1991) Dietary α-Linolenic Acid and Immunocompetence in Humans, Am. J. Clin Nutr. 53:40–46.

    PubMed  CAS  Google Scholar 

  5. Endres, S., Ghorbani, R., Kelley, V.E., Georgilis, K., Lonnemann, G., Van der Meer, J.W.M., Cannon, J.G., Rogers, T.S., Klempner, M.S., Webber, P.C., Schaeffer, E.J., Wolff, S.M., and Dinarello, C.A. (1989) The Effect of Dietary Supplementation with n-3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononuclear Cells, New Eng. J. Med. 320, 265–271.

    Article  PubMed  CAS  Google Scholar 

  6. Endres, S., Meydani, S.N., Ghorbani, R., Schindler, R., and Dinarello, C.A. (1993) Dietary Supplementation with n-3 Fatty Acids Suppresses Interleukin-2 Production and Mononuclear Cell Proliferation, J. Leukocyte Biol. 54, 599–603.

    PubMed  CAS  Google Scholar 

  7. Krammer, T.R., Schone, N., and Douglas, L.W. (1991) Increased Vitamin E Intake Restores Fish Oil-Induced Suppressed Blastogenesis of Mitogen-Stimulated T Lymphocytes, Am. J. Clin. Nutr. 54, 896–902.

    Google Scholar 

  8. Rasmussen, L.B., Kiens, B., Pederson, B.K., and Richter, E.A. (1994) Effect of Diet and Plasma Fatty Acid Composition on Immune Status of Elderly Men, Am. J. Clin. Nutr. 59, 572–577.

    PubMed  CAS  Google Scholar 

  9. Seyberth, H.W., Oelz, O., Kennedy, T., Sweetman, B.J., Danon, A., Frolich, J.C., Heimberg, M., and Oates, J.A. (1975) Increased Arachidonic Acid in Lipids After Administration to Man: Effects on Prostaglandin Biosynthesis, Clin. Pharmacol. Ther. 18, 521–529.

    PubMed  CAS  Google Scholar 

  10. O’Dea, K., and Sinclair, A. (1982) Increased Proportion of Arachidonic Acid in Plasma Lipids After Two Weeks on a Diet of Tropical Seafood, Am. J. Clin. Nutr. 36, 868–72.

    PubMed  CAS  Google Scholar 

  11. Whelan, J., Surette, M.E., Hardardottir, I., Lu, G., Golemboski, K.A., Larsen, E., and Kinsella, J.E. (1993) Dietary Arachidonic Acid Enhances Tissue Arachidonate Levels and Eicosanoid Production in Syrian Hamsters, J. Nutr. 123, 2174–2185.

    PubMed  CAS  Google Scholar 

  12. Aebischer, C.P., Pasche, I., and Jorg, A. (1993) Nanomolar Arachidonic Acid Influences the Respiratory Burst in Eosinophils and Neutrophils Induced by GTP-Binding Protein, Eur. J. Biochem. 218, 669–677.

    Article  PubMed  CAS  Google Scholar 

  13. Hubbard, N.E., Lim, D., Sommers, S.D., and Erickson, K.L. (1993) Effects of in vitro Exposure to Arachidonic Acid on TNF-α Production by Murine Peritoneal Macrophages, J. Leukocyte Biol. 54:105–110.

    PubMed  CAS  Google Scholar 

  14. Holladay, C.S., Wright, R.M., and Spangelo, B.L. (1993) Arachidonic Acid Stimulates Interleukin-6 Release from Rat Peritoneal Macrophages in vitro: Evidence for a Prostacyclin Dependent Mechanism, Prostaglandins, Leukotrienes Essent. Fatty Acids 49, 915–922.

    Article  CAS  Google Scholar 

  15. Gualde, N., Atluru, D., and Goodwin, J.S. (1985) Effect of Lipoxygenase Metabolites of Arachidonic Acid on Proliferation of Human T Cell Subsets, J. Immunol. 134, 1125–1129.

    PubMed  CAS  Google Scholar 

  16. Kunkel, S.L., and Chensue, S.W. (1986) The Role of Arachidonic Acid Metabolites in Mononuclear Phagocytic Cell Interactions, International J. Dermatol. 25, 83–89.

    CAS  Google Scholar 

  17. Takata, S., Papayianni, A., and Matsubara, M., Jimenez, W., Pronovost, P.H., and Brady, H.R. (1994) 15-Hydroxyeicosatetraenoic Acid Inhibits Neutrophil Migration Across Cytokine Activated Endothelium, Am. J. Pathol. 145, 541–549.

    PubMed  CAS  Google Scholar 

  18. Phinney, S.D., Odin, R.S., Johnson, S.B., and Holman, R.T. (1990) Reduced Arachidonate in Serum Phospholipids and Cholesterol Esters Associated with Vegetarian Diets in Humans, Am. J. Clin. Nutr. 51, 385–392.

    PubMed  CAS  Google Scholar 

  19. Garg, A., Bonanome, A., Grundy, S.M., Zhang, Z.M., and Linger, R.H. (1988) Comparison of a High-Carbohydrate Diet with a High-Monounsaturated Fat Diet in Patients with Non-insulin Dependent Diabetes Mellitus, N. Eng. J. Med. 319, 829–34.

    Article  CAS  Google Scholar 

  20. Baghurst, K.I., Crawford, D.A., Worsley, D.A., and Record, S.J. (1988) The Victorian-Nutrition Survey Intakes and Sources of Dietary Fats and Cholesterol in Victorian Population, Med. J. Aust. 149, 13–20.

    Google Scholar 

  21. Dolecek, T.A. (1992) Epidemiological Evidence of Relationships Between Dietary Polyunsaturated Fatty Acids and Mortality in the Multiple Risk Factor Intervention Trial, proc. Soc. Exp. Biol. Med. 200, 177–182.

    PubMed  CAS  Google Scholar 

  22. Hamazaki, T., Fischer, S., Urakazi, M., Sawazaki, S., Yano, S., and Kuwamori, T. (1989) Urinary Excretion of PGI2/PGI3 and Recent n-6/n-3 Fatty Acid Intake, Prostaglandins 37, 417–424.

    Article  PubMed  CAS  Google Scholar 

  23. Mann, N.M., Johnson, L.G., Warrick, G.E., and Sinclair, A.J. (1995) The Arachidonic Acid Content of the Australian Diet Is Lower Than Previously Estimated, J. Nutr. 125, 2528–2535.

    PubMed  CAS  Google Scholar 

  24. Nelson, G.J., Kelley, D.S., Emken, E.A., Phinney, S.D., Kyle, D., and Ferretti, A. (1997) A Human Dietary Arachidonic Acid Supplementation Study Conducted in a Metabolic Research Unit: Rationale and Design, Lipids 32, 415–420.

    PubMed  CAS  Google Scholar 

  25. Kelley, D.S., Daudu, P.A., Taylor, P.C., Mackey, B.E., and Turnlund, J.R. (1995) Effects of Low-Copper Diets on Human Immune Response, Am. J. Clin. Nutr. 62, 412–416.

    PubMed  CAS  Google Scholar 

  26. SAS Institute Inc., SAS/STAT ™ (1987) Guide for Personal Computers, Version 6 edn., SAS Institute Inc., Cary, NC.

    Google Scholar 

  27. Williams, T.J., and Peck, M.J. (1977) Role of Prostaglandin Mediated Vasodilation in Inflammation, Nature 270, 530–532.

    Article  PubMed  CAS  Google Scholar 

  28. Soter, N.A., Lewis, R.A., Korey, E.J., and Austen, K.F. (1983) Local Effects of Synthetic Leukotrienes in Human Skin, J. Invest. Dermatol. 80, 115–119.

    Article  PubMed  CAS  Google Scholar 

  29. Camp, R.D.R., Coutts, A.A., Greaves M.W., Kay, A.B., and Walport, M.J. (1983) Responses of Human Skin to Intradermal Injection of Leukotrienes, J. Pharmacol. 80, 497–502.

    CAS  Google Scholar 

  30. Heslett, C., Worthen, G.S., Giclas, P., Morrison, D.C., Henson, J.E., and Henson, P.M. (1987) The Pulmonary Vascular Sequestration of Neutrophils in Endotoxemia Is Initiated by an Effect of Endotoxin on the Neutrophil, Am. Rev. Respir. Dis. 136, 9–15.

    Google Scholar 

  31. McCall, C.E., De Chatelet, L.R., Brown, L.R., and Lachmann, P. (1974) New Biological Activity Following Intravascular Activation of Complement System, Nature 249, 841–844.

    Article  PubMed  CAS  Google Scholar 

  32. Muir, A.L., Cruz, M., Martin, B.A., Thommasen, H., Belzberg, A., and Hogg, J.C. (1984) Leukocyte Kinetics in Human Lung: Role of Exercise and Catecholamines, J. Appl. Physiol. 57, 711–716.

    PubMed  CAS  Google Scholar 

  33. Doukas, J., Hechman, H.B., and Shepro, D. (1988) Endothelial Secreted Arachidonic Acid Metabolies Modulate Polymorphonuclear Leukocyte Chemotaxis and Diapedesis, Blood 71, 771–779.

    PubMed  CAS  Google Scholar 

  34. Cook, R.G., Stavitsky, A.B., and Harold, W.W. (1978) Regulation of in vitro Anamnestic Antibody Response by Cyclic AMP. II. Antigen Dependent Enhancement by Exogenous Prostaglandins of the E Series, Cell. Immunol. 40, 128–140.

    Article  PubMed  CAS  Google Scholar 

  35. Goodwin, J.S., Selinger, G.S., Messner, R.P., and Reed, W.P. (1978) Effect of Indomethacin in vivo on Humoral and Cellular Immunity in Humans, Infec. Immun. 19, 430–433.

    CAS  Google Scholar 

  36. Kelly, J.P., and Parker, C.W. (1978) Effect of Arachidonic Acid and Other Unsaturated Fatty Acids on Human Lymphocyte Transformation in vitro, Int. Arch. Allergy Appl. Immunol. 122, 1560–1562.

    Google Scholar 

  37. Kumar, G.S., and Das, U.N. (1994) Effect of Prostaglandins and Their Precursors on the Proliferation of Human Lymphocytes and Their Secretion of Tumor Necrosis Factor and Various Interleukins, Prostaglandins Leukotrienes Essent. Fatty Acids 50, 331–334

    Article  CAS  Google Scholar 

  38. Nokta, M.A., Hassan, M.I., Loesch, K.A., and Pollard, R.B. (1995) HIV-Induced TNF-α Regulates Arachidonic Acid and PGE2 Release from HIV-Infected Mononuclear Phagocytes, Virology 208, 590–600.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Western Regional Research Center, USDA, ARS, Albany, California

    Bruce E. Mackey

  2. USDA, ARS Martek Corporation, 21045, Columbia, Maryland

    David Kyle

  3. Western Human Nutrition, ARS, USDA, Presidio of San Francisco, P.O. Box 29997, 94129, CA, Research Center

    Darshan S. Kelley, Peter C. Taylor, Gary J. Nelson & Perla C. Schmidt

Authors
  1. Darshan S. Kelley
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  2. Peter C. Taylor
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Kelley, D.S., Taylor, P.C., Nelson, G.J. et al. Effects of dietary arachidonic acid on human immune response. Lipids 32, 449–456 (1997). https://doi.org/10.1007/s11745-997-0059-3

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  • DOI: https://doi.org/10.1007/s11745-997-0059-3

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