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Lipid Rafts Promote trans Fatty Acid-Induced Inflammation in Human Umbilical Vein Endothelial Cells

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Lipids

Abstract

The effects of two fatty acids, oleic acid (OLA) and elaidic acid (ELA) on normal human umbilical vein endothelial cells (HUVEC) and non-rafts HUVEC were investigated in this study. The expression levels of inflammatory cytokines (ICAM-1, VCAM-1 and IL-6) were analyzed. Western blot was used to analyze the expression levels of inflammation-related proteins (NF-κB, ERK1/2) and toll-like receptors 4 (TLR4). The results showed that the levels of nuclear translocation of NF-κB p65 and phosphorylated ERK1/2 were significantly decreased only in non-lipid rafts cells pretreated with trans fatty acid (TFA). The expression of TLR4 in the ELA-treated normal cells was higher than that in non-lipid rafts HUVEC. When the lipid rafts was destroyed by methyl-β-cyclodextrin, the levels of nuclear translocation of NF-κB p65, phosphorylated ERK1/2 and TLR4 were decreased significantly. Therefore, lipid rafts may be involved in TFA induced-inflammation in HUVEC through blocking the inflammatory signal pathway. Lipid rafts might be a platform for specific receptors such as TLR4 for TFA to activate the pro-inflammation on cell membranes.

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Abbreviations

DMEM:

Dulbecco’s modified eagle medium

ELA:

Elaidic acid

EC:

Endothelial cell(s)

FBS:

Fetal bovine serum

HUVEC:

Human umbilical vein endothelial cell(s)

ICAM-1:

Intercellular adhesion molecule-1

IL-6:

Interleukin-6

MβCD:

Methyl-β-cyclodextrin

OLA:

Oleic acid

PBS:

Phosphate buffered saline

TFA:

trans Fatty acid(s)

TLR4:

Toll-like receptor 4

VCAM-1:

Vascular cell adhesion molecule-1

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Acknowledgements

This project was funded by the National Natural Science Foundation of China (NO. 31060214 and NO. 31401485) and Natural Science Foundation of Jiangxi Province (NO. 20152ACB20001).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, 330047, Jiangxi, China

    Yao Pan, Benxin Liu, Zeyuan Deng, Yawei Fan, Jing Li & Hongyan Li

  2. Institute for Advanced Study, University of Nanchang, Nanchang, 330031, Jiangxi, China

    Zeyuan Deng

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  1. Yao Pan
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  2. Benxin Liu
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  3. Zeyuan Deng
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Correspondence to Hongyan Li.

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Pan, Y., Liu, B., Deng, Z. et al. Lipid Rafts Promote trans Fatty Acid-Induced Inflammation in Human Umbilical Vein Endothelial Cells. Lipids 52, 27–35 (2017). https://doi.org/10.1007/s11745-016-4213-2

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  • DOI: https://doi.org/10.1007/s11745-016-4213-2

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