Abstract
Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles attenuates glucose uptake and is closely related to the pathogenesis of type 2 diabetes. Therefore, up-regulation of DGKδ expression is thought to protect and improve glucose homoeostasis in type 2 diabetes. We recently determined that myristic acid (14:0), but not palmitic (16:0) or stearic (18:0) acid, significantly increased DGKδ2 protein expression in mouse C2C12 myotubes. In the current study, we analyzed whether myristic acid indeed enhances glucose uptake in C2C12 myotubes. We observed that myristic acid caused ~1.4-fold increase in insulin-independent glucose uptake. However, palmitic and stearic acids failed to enhance glucose uptake. DGKδ-specific siRNA decreased myristic acid-dependent increase of glucose uptake. Moreover, overexpression of DGKδ2 enhanced glucose uptake in C2C12 cells in the absence of myristic acid treatment. Taken together, these results strongly suggest that myristic acid enhances basal glucose uptake in myotubes in a DGKδ2 expression-dependent manner.
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Abbreviations
- BSA:
-
Bovine serum albumin
- DG:
-
Diacylglycerol
- DGK:
-
Diacylglycerol kinase
- 2-NBDG:
-
2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose
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Acknowledgments
This work was supported by MEXT/JSPS KAKENHI Grant Nos. 22370047 [Grant-in-Aid for Scientific Research (B)], 23116505 (Grant-in-Aid for Scientific Research on Innovative Areas), 25116704 (Grant-in-Aid for Scientific Research on Innovative Areas), 26291017 [Grant-in-Aid for Scientific Research (B)], and 15K14470 (Grant-in-Aid for Challenging Exploratory Research), the Japan Science and Technology Agency (AS221Z00794F, AS231Z00139G, AS251Z01788Q, and AS2621643Q), the Naito Foundation, the Hamaguchi Foundation for the Advancement of Biochemistry, the Daiichi-Sankyo Foundation of Life Science, the Terumo Life Science Foundation, the Futaba Electronic Memorial Foundation, the Daiwa Securities Health Foundation, the Ono Medical Research Foundation, the Japan Foundation for Applied Enzymology, the Food Science Institute Foundation, the Skylark Food Science Institute and the Venture Business Laboratory of Chiba University (FS).
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Wada, Y., Sakiyama, S., Sakai, H. et al. Myristic Acid Enhances Diacylglycerol Kinase δ-Dependent Glucose Uptake in Myotubes. Lipids 51, 897–903 (2016). https://doi.org/10.1007/s11745-016-4162-9
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DOI: https://doi.org/10.1007/s11745-016-4162-9