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Lipoprint Adequately Estimates LDL Size Distribution, but not Absolute Size, Versus Polyacrylamide Gradient Gel Electrophoresis

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  • Published:
Lipids

Abstract

Recently, a new cost-effective and less labor-intensive technique termed the “lipoprint LDL system” was developed to measure LDL particle size. However, the agreement between lipoprint and previously validated techniques, such as polyacrylamide gradient gel electrophoresis (PGGE), has never been tested. Therefore, we measured LDL size by lipoprint and PGGE in 16 obese subjects at 4 different time points. Lipoprint significantly overestimated (P = 0.003) integrated LDL particle size by 1.1 ± 3.0 Å when compared to PGGE. As for distribution, there was good agreement between methods for the estimation of large, medium, and small particles (mean difference between the methods was <3% for each parameter). Correlational analysis also revealed good relationships between methods for the proportion of large (r = 0.81, P < 0.0001), medium (r = 0.67, P < 0.0001), and small (r = 0.73, P < 0.0001) particles. In sum, although there is good agreement between lipoprint and PGGE for the determination of LDL size distribution, absolute LDL size values may differ between the two methods.

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Fig. 1

Abbreviations

PGGE:

Polyacrylamide gradient gel electrophoresis

CHD:

Coronary heart disease

NMR:

Nuclear magnetic resonance

BMI:

Body mass index

CV:

Coefficient of variation

TE:

Technical error of measurement

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Acknowledgments

We would like to thank the study participants who contributed their time and effort. Departmental grant, Kinesiology and Nutrition, University of Illinois, Chicago.

Conflicts of interest

The authors have no conflicts of interest to report.

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Authors and Affiliations

  1. Department of Kinesiology and Nutrition, University of Illinois at Chicago, 1919 West Taylor Street, Room 506F, Chicago, IL, 60612, USA

    Krista A. Varady

  2. Nutraceuticals and Functional Foods Institute, University of Laval, Ste-Foy, QC, Canada

    Benoît Lamarche

Authors
  1. Krista A. Varady
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  2. Benoît Lamarche
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Correspondence to Krista A. Varady.

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Varady, K.A., Lamarche, B. Lipoprint Adequately Estimates LDL Size Distribution, but not Absolute Size, Versus Polyacrylamide Gradient Gel Electrophoresis. Lipids 46, 1163–1167 (2011). https://doi.org/10.1007/s11745-011-3611-8

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  • DOI: https://doi.org/10.1007/s11745-011-3611-8

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