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Modulation of Platelet Aggregation, Haematological and Histological Parameters by Structured Lipids on Hypercholesterolaemic Rats

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Lipids

Abstract

The effect of the consumption of medium-chain fatty acid (MCFA)-rich and polyunsaturated fatty acid (PUFA)-rich mustard oil on platelet aggregation, haematological parameters and the liver was studied in male albino rats. The rats were fed on standard stock diet with control (mustard oil) and experimental oils for 28 days. Haematological examinations in the normal condition showed that there was no significant variation in the platelet count, total white blood cell (WBC) and red blood cell (RBC) counts, haematocrit value and mean cell haemoglobin concentration (MCHC) percentage in the rats fed with control and experimental oils. Haematological examinations in the hypercholesterolaemic condition revealed that there was a significant increase in the platelet count by 39.38% in hypercholesterolaemia, which was decreased by 27.29 and 42.71% by the administration of the experimental oils, respectively. The haemoglobin level was decreased by 5.3%, whereas the haematocrit value was increased by 12.52% in hypercholesterolaemia, which were normalised by treatment with the experimental oils. The platelet aggregation study indicated that the adenosine diphosphate (ADP)-induced platelet aggregation increased by 71.67% in hypercholesterolaemia, but the experimental oils beneficially reduced platelet aggregation by 26.33 and 68.33%, respectively. There was increased total cholesterol, non-high-density lipoprotein (non-HDL) cholesterol and triglyceride levels in liver in hypercholesterolaemia, which was also recovered by the administration of experimental oils. Organopathological examination showed that there was deposition of cholesterol in the liver in the hypercholesterolaemic condition, which was also reduced by treatment with the two experimental oils.

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Abbreviations

ADP:

Adenosine diphosphate

AMRD:

Age-related macular degeneration

DHA:

Docosahexaenoic acid

EPA:

Eicosapentaenoic acid

EPA–LA:

Eicosapentaenoate–lipoate

HDL:

High-density lipoprotein

LCT:

Long-chain triglyceride

MCT:

Medium-chain triglyceride

MCHC:

Mean cell haemoglobin concentration

MCV:

Mean corpuscular volume

PRP:

Platelet-rich plasma

TAG:

Triacylglycerol

PUFA:

Polyunsaturated fatty acid

ROS:

Reactive oxygen species

5-HT:

5-Hydroxytryptamine

References

  1. Ross R (1999) Mechanisms of disease: atherosclerosis—an inflammatory disease. N Eng J Med 340:115–126

    Article  CAS  Google Scholar 

  2. Kojda G, Harrison DG (1999) Interactions between NO and reactive oxygen species: pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc Res 43:562–571

    Article  CAS  PubMed  Google Scholar 

  3. Aviram M, Brook JG (1987) Platelet activation by plasma lipoproteins. Prog Cardiovasc Dis 30:61–72

    Article  CAS  PubMed  Google Scholar 

  4. Harrison DG (1997) Cellular and molecular mechanisms of endothelial cell dysfunction. J Clin Invest 100(9):2153–2157

    Article  CAS  PubMed  Google Scholar 

  5. Kugiyama K, Kerns SA, Morrisett JD, Roberts R, Henry PD (1990) Impairment of endothelium-dependent arterial relaxation by lysolecithin in modified low-density lipoproteins. Nature 344:160–162

    Article  CAS  PubMed  Google Scholar 

  6. Steinberg D (1997) Lewis A. Conner memorial lecture. Oxidative modification of LDL and atherogenesis. Circulation 95:1062–1071

    CAS  PubMed  Google Scholar 

  7. Tremoli E, Colli S, Maderna P, Baldassarre D, Di Minno G (1993) Hypercholesterolemia and platelets. Semin Thromb Hemost 19:115–121

    Article  CAS  PubMed  Google Scholar 

  8. Willoughby S, Holmes A, Loscalzo J (2002) Platelets and cardiovascular disease. Eur J Cardiovasc Nurs 1:273–288

    Article  PubMed  Google Scholar 

  9. Napolitano M, Rivabene R, Avella M, Amicone L, Tripodi M, Botham KM, Bravo E (2001) Oxidation affects the regulation of hepatic lipid synthesis by chylomicron remnants. Free Radic Biol Med 30(5):506–515

    Article  CAS  PubMed  Google Scholar 

  10. Napoli C, Lerman LO (2001) Involvement of oxidation-sensitive mechanisms in the cardiovascular effects of hypercholesterolemia. Mayo Clin Proc 76:619–631

    Article  CAS  PubMed  Google Scholar 

  11. Kumar SA, Sudhahar V, Varalakshmi P (2006) Protective role of eicosapentaenoate–lipoate (EPA–LA) derivative in combating oxidative hepatocellular injury in hypercholesterolemic atherogenesis. Atherosclerosis 189:115–122

    Article  CAS  PubMed  Google Scholar 

  12. Bach AC, Babayan VK (1982) Medium-chain triglycerides: an update. Am J Clin Nutr 36:950–962

    CAS  PubMed  Google Scholar 

  13. Drevon CA, Baksaas I, Krokan HE (eds) (1993) Omega-3 fatty acids: metabolism and biological effects. Birkhauser Verlag, Basel, Switzerland, 389 pp

    Google Scholar 

  14. Simopoulos AP, Leaf A, Salem N Jr (1999) Essentiality of and recommended dietary intakes for omega-6 and omega-3 fatty acids. Ann Nutr Meta 43:127–130

    Article  CAS  Google Scholar 

  15. Soei LK, Lamers JMJ, Sassen LMA, van Tol A, Scheek LM, Dekkers DHW, van Meegen J, Verdouw P (1995) Fish oil: a modulator of experimental atherosclerosis in animals. In: Kristensen SD, Schmidt EB, De Caterina R, Endres S (eds) n-3 fatty acids: prevention and treatment of vascular disease. Bi & Gi Publishers, Verona, pp 55–75

    Google Scholar 

  16. Bailey AE (1951) Industrial oil and fat products. Interscience Publishers, New York

    Google Scholar 

  17. Metcalfe LD, Schmitz AA, Pelka JR (1966) Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Chem 38:514–515

    Article  CAS  Google Scholar 

  18. Paul W, Queen LR, Page CP, Ferro A (2007) Increased platelet aggregation in vivo in the Zucker Diabetic Fatty rat: differences from the streptozotocin diabetic rat. Br J of Pharmacol 150:105–111

    Article  CAS  Google Scholar 

  19. Folch J, Ascoli I, Lees M, Meath JA, LeBaron N (1951) Preparation of lipid extracts from brain tissue. J Biol Chem 191:833–841

    CAS  PubMed  Google Scholar 

  20. Edem DO (2009) Haematological and histological alterations induced in rats by palm oil-containing diets. Eur J Sci Res 32(3):405–418

    Google Scholar 

  21. Babayan VK (1981) Medium chain length fatty acid esters and their medical and nutritional applications. J Am Oil Chem Soc 58:49A–51A

    Article  CAS  Google Scholar 

  22. Fushiki T, Matsumoto K, Inoue K, Kawada T, Sugimoto E (1995) Swimming endurance capacity of mice is increased by chronic consumption of medium-chain triglycerides. J Nutr 125(3):531–539

    CAS  PubMed  Google Scholar 

  23. Balasubramaniam S, Simons LA, Chang S, Hickie JB (1985) Reduction in plasma cholesterol and increase in biliary cholesterol by a diet rich in n-3 fatty acids in the rat. J Lipid Res 26(6):684–689

    CAS  PubMed  Google Scholar 

  24. Hromadová M, Seböková E, Klimeś I (1994) HMG-CoA reductase activity in the liver of rats with hereditary hypertriglyceridemia: effect of dietary fish oil. Endocr Regul 28:211–214

    PubMed  Google Scholar 

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Acknowledgements

The work was funded by the University of Calcutta under the scheme “University with Potential for Excellence” funded by Government of India. Sincere thanks are also due for Dr. Biswajit Bhattacharya, Medical Officer, Blood Bank, R. G. Kar Medical College and Hospital, Kolkata, and Dr. (Ms.) Maitreyee Bhattacharya and Dr. Sanjoy Misra, Department of Haematology, N.R.S. Medical College and Hospital, Kolkata, for their help in finding out the haematological and platelet aggregation parameters, respectively, performed in the above study. We are very thankful to Prof. D. K. Bhattacharyya, Ex-Professor Emeritus, Department of Chemical Technology, University of Calcutta, for his wonderful mentorship.

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  1. Department of Chemical Technology, University College of Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India

    Avery Sengupta & Mahua Ghosh

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  1. Avery Sengupta
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  2. Mahua Ghosh
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Correspondence to Mahua Ghosh.

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Sengupta, A., Ghosh, M. Modulation of Platelet Aggregation, Haematological and Histological Parameters by Structured Lipids on Hypercholesterolaemic Rats. Lipids 45, 393–400 (2010). https://doi.org/10.1007/s11745-010-3411-6

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  • DOI: https://doi.org/10.1007/s11745-010-3411-6

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