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Effect of a Seaweed Extract on Fatty Acid Accumulation and Glycerol-3-Phosphate Dehydrogenase Activity in 3T3-L1 Adipocytes

  • Original Article
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Lipids

Abstract

This study was to determine the effect of a seaweed Ascophyllum nodosum extract (SE) containing 220 mg g−1 phlorotannins on differentiation and fatty acid accumulation in differentiating 3T3-L1 adipocytes. 3T3-L1 cells (2 × 104 mL−1) were seeded to 24-well plates and proliferated to reach confluence and then were treated with media containing 0, 12.5, 25, 50, 75 and 100 μg mL−1 SE for 8 days. Dexamethasone, methyl-isobutylxanthine and insulin (DMI) were added to the media in the first 2 days to induce cell differentiation. On day 8 the adipocytes were harvested for measuring cellular fatty acid concentration and the activity of glycerol-3-phosphate dehydrogenase (GPDH). It was found that treatment with SE increased (P < 0.01, n = 6) cellular myristoleic acid (C14:1), palmitoleic acid (C16:1) and oleic acid (C18:1) and total monounsaturated fatty acids (MUFA) without significantly affecting the cell number and saturated fatty acid (SFA). Ratios of MUFA/SFA, C14:1/C14:0, C16:1/C16:0 and C18:1/C18:0 in cellular lipids increased (P < 0.05, n = 6) with the SE treatment in a dose dependent manner (P < 0.001). Treatment with 75 μg mL−1 SE depressed (P < 0.05) cellular GPDH activity. The results indicate that the biological factors in the SE may be involved in differentiation and MUFA accumulation in adipocytes.

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Abbreviations

DHAP:

Dihydroxyacetone phosphate

DMEM:

Dulbecco’s modified Eagle’s medium

DMI:

Dexamethasone, methyl-isobutylxanthine and insulin

FA:

Fatty acids

FBS:

Fetal bovine serum

GPDH:

Glycerol-3-phosphate dehydrogenase

MUFA:

Monounsaturated fatty acids

NADH:

β-Nicotinamide adenine dinucleotide, reduced form

PBS:

Phosphate buffered saline

SFA:

Saturated fatty acids

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Acknowledgments

M.L.H. thanks B. Pink and K. Jakober for their help in checking and revising the manuscript. The manuscript is Lethbridge Research Centre contribution #387-08046.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada

    M. L. He, Y. Wang, J. S. You, P. S. Mir & T. A. McAllister

  2. Department of Agriculture, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada

    M. L. He

  3. Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, Liaoning, China

    J. S. You

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  1. M. L. He
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Correspondence to M. L. He.

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He, M.L., Wang, Y., You, J.S. et al. Effect of a Seaweed Extract on Fatty Acid Accumulation and Glycerol-3-Phosphate Dehydrogenase Activity in 3T3-L1 Adipocytes. Lipids 44, 125–132 (2009). https://doi.org/10.1007/s11745-008-3256-4

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  • DOI: https://doi.org/10.1007/s11745-008-3256-4

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