Skip to main content
SpringerLink
Log in

Effect of Dietary Cholesterol and Fat on Cell Cholesterol Transfer to Postprandial Plasma in Hyperlipidemic Men

  • Original Articel
  • Published:
Lipids

Abstract

Postprandial chylomicrons are potent ultimate acceptors of cell membrane cholesterol and are believed to accelerate reverse cholesterol transport (RCT). We compared the effects of meals rich in polyunsaturated fat (PUFA) and either high (605 mg) or low (151 mg) in cholesterol and a meal rich in dairy fat (DF) in the form of cream on net in vitro transport of red blood cell (RBC) membrane cholesterol to 4 and 6 h postprandial plasma in eight normotriglyceridemic (NTG-H) and eight hypertriglyceridemic (HTG-H) men with mild to moderate hypercholesterolemia. In HTG-H men, cell cholesterol accumulation in 6-h postprandial plasma was significantly (P = 0.02) less after the PUFA-HC meal compared with the other meals. The significant (P < 0.001) increase in cell plus endogenous cholesterol accumulation in the triglyceride-rich lipoprotein (TRL) fraction of 4 h postprandial plasma incubated with RBC was significantly (P = 0.007) higher after the PUFA-HC meal compared with DF meal in HTG-H men. In NTG-H men, cholesterol accumulation in plasma and plasma lipoproteins in the presence and absence of RBC was not significantly affected by the type of meal ingested. These data suggest that addition of large amounts of cholesterol to a PUFA meal may impair diffusion-mediated transport of cell membrane cholesterol to postprandial plasma and that replacing DF with PUFA in a meal increases postprandial lipemia and may potentially increase cholesterol accumulation in atherogenic postprandial TRL in HTG-H men.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

apoB:

Apolipoprotein B

ABCA1:

ATP binding cassette A1

ABCG1:

ATP binding cassette G1

CE:

Cholesteryl esters

CETP:

Cholesteryl ester transfer protein

DF:

Dairy fat

HDL:

High density lipoprotein

HTG-H:

Hypertriglyceridemic and hypercholesterolemic

LCAT:

Lecithin:cholesterol acyltransferase

LDL:

Low density lipoprotein

MUFA:

Monounsaturated fatty acids

NTG-H:

Normotriglyceridemic and hypercholesterolemic

PUFA-HC:

Polyunsaturated fatty acid-high cholesterol

PUFA-LC:

Polyunsaturated fatty acid-low cholesterol

RBC:

Red blood cells

RCT:

Reverse cholesterol transport

SAFA:

Saturated fatty acids

SRB1:

Scavenger receptor B1

TG:

Triglycerides

TC:

Total cholesterol

TRL:

Triglyceride-rich lipoproteins

UC:

Unesterified cholesterol

VLDL:

Very low density lipoproteins

References

  1. Yancey PG, Bortnick AE, Kellner-Weibel G, de la Llera-Moya M, Phillips MC, Rothblat GH (2003) Importance of different pathways of cellular cholesterol efflux. Arterioscler Thromb Vasc Biol 23:712–719

    Article  CAS  PubMed  Google Scholar 

  2. Cavelier C, Lorenzi I, Rohrer L, von Eckardstein A (2006) Lipid efflux by the ATP-binding cassette transporters ABCA1 and ABCG1. Biochim Biophys Acta 1761:655–666

    CAS  PubMed  Google Scholar 

  3. Duong MN, Collins HL, Jin W, Zanotti I, Favari E, Rothblat GH (2006) Relative contributions of ABCA1 and SR-B1 to cholesterol efflux to serum from fibroblasts and macrophages. Arterioscler Thromb Vasc Biol 26:541–547

    Article  CAS  PubMed  Google Scholar 

  4. Schwartz CC, VandenBroek JM, Cooper PS (2004) Lipoprotein cholesteryl ester production, transfer, and output in vivo in humans. J Lipid Res 45:1594–1607

    Article  CAS  PubMed  Google Scholar 

  5. Fielding CJ, Fielding PE (1995) Molecular physiology of reverse cholesterol transport. J Lipid Res 36:211–228

    CAS  PubMed  Google Scholar 

  6. Castro GR, Fielding CJ (1985) Effects of postprandial lipemia on plasma cholesterol metabolism. J Clin Invest 75:874–882

    Article  CAS  PubMed  Google Scholar 

  7. Chung BH, Franklin F, Cho BHS, Segrest JP, Hart K, Darnell BE (1998) Potencies of lipoproteins in fasting and postprandial plasma to accept additional cholesterol molecules released from cell membranes. Arterioscler Thromb Vasc Biol 18:1217–1230

    CAS  PubMed  Google Scholar 

  8. Chung BH, Liang P, Doran S, Simon Cho BH, Franklin F (2004) Postprandial chylomicrons: potent vehicles for transporting cholesterol from endogenous LDL + HDL and cell membranes to the liver via LCAT and CETP. J Lipid Res 45:1242–1255

    Article  CAS  PubMed  Google Scholar 

  9. Dubois C, Armand M, Mekki N, Portugal H, Pauli A-M, Bernard P-M, Lafont H, Lairon D (1994) Effects of increasing amounts of dietary cholesterol on postprandial lipemia and lipoproteins in human subjects. J Lipid Res 35:1993–2007

    CAS  PubMed  Google Scholar 

  10. Mekki N, Charbonnier M, Borel P, Leonardi J, Juhel C, Portugal H, Lairon D (2002) Butter differs from olive oil and sunflower oil in its effects on postprandial lipemia and triacylglycerol-rich lipoproteins after single mixed meals in healthy young men. J Nutr 132:3642–3649

    CAS  PubMed  Google Scholar 

  11. Tholstrup T, Sandström B, Bysted A, Holmer G (2001) Effect of 6 dietary fatty acids on the postprandial lipid profile, plasma fatty acids, lipoprotein lipase and cholesterol ester transfer activities in healthy young men. Am J Clin Nutr 73:198–208

    CAS  PubMed  Google Scholar 

  12. Fielding PE, Jackson EM, Fielding CJ (1989) Chronic fat and cholesterol inhibit the normal postprandial stimulation of plasma cholesterol metabolism. J Lipid Res 30:1211–1217

    CAS  PubMed  Google Scholar 

  13. Guerin M, Egger P, Soudant C, Le Goff W, van Tol A, Dupuis R, Chapman MJ (2002) Cholesteryl ester flux from HDL to VLDL-1 is preferentially enhanced in type IIB subjects in the postprandial state. J Lipid Res 43:1652–1660

    Article  CAS  PubMed  Google Scholar 

  14. Knopp RH, Retzlaff BM, Walden CE, Dowdy AA, Tsunehara CH, Austin MA, Nguyen T (1997) A double-blind, randomized, controlled trial of the effects of two eggs per day in moderately hypercholesterolemic and combined hyperlipidemic subjects taught the NCEP step I diet. J Am Coll Nutr 16:551–561

    CAS  PubMed  Google Scholar 

  15. National Institutes of Health: Executive Summary (2001) In: Third report of the national cholesterol education program expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III). Washington, DC, US Govt. Printing Office, 2001 (NIH publ. no. 01–3670)

  16. Warnick GR, Benderson JA, Albers JJ (1982) Dextran-sulphate-Mg2+ precipitation procedure for quantification of high density lipoprotein cholesterol. Clin Chem 28:1379–1388

    CAS  PubMed  Google Scholar 

  17. Albers JJ, Chen CH, Adolphson JL (1981) Lecithin: cholesterol acyltransferase (LCAT) mass; its relationship to LCAT activity and cholesterol esterification rate. J Lipid Res 22:1206–1213

    CAS  PubMed  Google Scholar 

  18. Czarnecka H, Yokoyama S (1996) Regulation of cellular cholesterol efflux by lecithin:cholesterol acyltransferase reaction through nonspecific lipid exchange. J Biol Chem 271:2023–2028

    Article  CAS  PubMed  Google Scholar 

  19. Guérin M, Bruckert E, Dolphin PJ, Chapman MJ (1996) Absence of cholesteryl ester transfer protein-mediated cholesteryl ester mass transfer from high-density lipoprotein to low-density lipoprotein particles is a major feature of combined hyperlipidaemia. Eur J Clin Invest 26:485–494

    Article  PubMed  Google Scholar 

  20. Karpe F, Tornvall P, Olivercrona T, Steiner G, Carlson LA, Hamsten A (1993) Composition of human low density lipoprotein: effects of postprandial triglyceride-rich lipoproteins, lipoprotein lipase, hepatic lipase and cholesteryl ester transfer protein. Atherosclerosis 98:33–49

    Article  CAS  PubMed  Google Scholar 

  21. Braaco U (1994) Effect of triglyceride structure on fat absorption. Am J Clin Nutr 60(suppl):1002S–1009S

    Google Scholar 

  22. Lassel TS, Guerin M, Auboiron S, Guy-Grand B, Chapman MJ (1999) Evidence for a cholesteryl ester donor activity of LDL particles during alimentary lipemia in normolipidemic subjects. Atherosclerosis 147:41–48

    Article  CAS  PubMed  Google Scholar 

  23. Chung B-H, Simon Cho BH, Liang P, Doran S, Osterlund L, Oster RA, Darnell B, Franklin F (2004) Contribution of postprandial lipemia to the dietary fat-mediated changes in endogenous lipoprotein–cholesterol concentrations in humans. Am J Clin Nutr 80:1145–1158

    CAS  PubMed  Google Scholar 

  24. Havel RJ (2000) Remnant lipoproteins as therapeutic targets. Curr Opin Lipidol 11:615–620

    Article  CAS  PubMed  Google Scholar 

  25. Patsch JR, Miesenböck G, Hofpfenweiser T, Muhlberger V, Knapp E, Dunn JK, Gotto AM Jr, Patsch W (1992) Relation of triglyceride metabolism and coronary artery disease in normolipidemic men with and without coronary artery disease: studies in post-prandial state. Arterioscler Thromb 12:1336–1345

    CAS  PubMed  Google Scholar 

  26. Weintraub MS, Grosskopf I, Rassin T, Miller H, Charach G, Rotmensch HH, Liron M, Rubenstein A, Iaina A (1996) Clearance of chylomicron remnants in normolipidemic patients with coronary artery disease: case control study over three years. BMJ 312:936–939

    CAS  PubMed  Google Scholar 

  27. Guérin M, Lassel TS, Le Goff W, Farnier M, Chapman MJ (2000) Action of atorvastatin in combined hyperlipidemia: preferential reduction of cholesteryl ester transfer from HDL to VLDL1 particles. Arterioscler Thromb Vasc Biol 20:189–197

    PubMed  Google Scholar 

  28. Castro Cabezas M, de Bruin TWA, Jansen H, Kock LAW, Kortland W, Erkelens DW (1993) Impaired chylomicron remnant clearance in familial combined hyperlipidemia. Arterioscler Thromb 13:804–814

    Google Scholar 

  29. Bergeron N, Havel RJ (1995) Influence of diets-rich in saturated fat and omega-6 polyunsaturated fatty acids on the postprandial response of apolipoproteins B-48, B-100, E, and lipids in triglyceride-rich lipoproteins. Arterioscler Thromb Vasc Biol 15:2111–2121

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the participants in the study. We are also grateful to Wendy Aitken in the Department of Human Nutrition for help in recruiting subjects. This study was supported by a Laurenson Award from the Otago Medical School Research Foundation.

Author information

Authors and Affiliations

  1. Department of Medical and Surgical Sciences, Dunedin School of Medicine, University of Otago, P.O. Box 913, Dunedin, 9054, New Zealand

    Wayne H. F. Sutherland, Sylvia A. de Jong & Robert J. Walker

Authors
  1. Wayne H. F. Sutherland
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sylvia A. de Jong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert J. Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wayne H. F. Sutherland.

About this article

Cite this article

Sutherland, W.H.F., de Jong, S.A. & Walker, R.J. Effect of Dietary Cholesterol and Fat on Cell Cholesterol Transfer to Postprandial Plasma in Hyperlipidemic Men. Lipids 42, 901–911 (2007). https://doi.org/10.1007/s11745-007-3101-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-007-3101-1

Keywords

Search

Navigation