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Effect of diacylglycerol on the development of impaired glucose tolerance in sucrose-fed rats

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Lipids

Abstract

The effects of DAG oil and TAG oil on impaired glucose tolerance in rats that were fed a diet containing high levels of sucrose were compared. Male Wistar rats (8 wk old and 32 wk old) were fed either high-sucrose (57.5% sucrose w/w) or control diets containing either 10% (w/w) DAG or TAG oil with a similar FA composition for 48 wk in 8-wk-old rats and for 24 wk in 32-wk-old rats. Plasma lipids, the size of the islets of Langerhans, and insulin, glucose, and adipocytokine levels were measured. An oral glucose tolerance test (OGTT) was carried out during the study period. For rats in both age groups that were fed a high-sucrose diet, the DAG oil group had lower plasma glucose and insulin response in an OGTT, and lower homeostasis model assessment-R levels, than the TAG oil group. Furthermore, in 8-wk-old rats that were fed a high-sucrose diet, the DAG oil group accumulated less visceral fat and showed decreases of plasma adiponectin and suppressed increases of plasma insulin, leptin, and the size of islet of Langerhans compared with the TAG oil group. No difference in the OGTT was found between the DAG and TAG oil groups in either age group of rats fed a control diet. In conclusion, these results suggest that DAG oil ingestion prevents the high-sucrose-diet-induced development of impaired glucose tolerance compared with TAG oil ingestion.

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Abbreviations

EIA:

enzyme-linked immunosorbent assay

HbA1c :

glycohemoglobin A1c

HOMA-R:

homeostasis model assessment-R

NEFA:

nonesterified FA

OGTT:

oral glucose tolerance test

OLETF:

Otsuka Long-Evans Tokushima Fatty

SD:

Sprague-Dawley

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Authors and Affiliations

  1. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, 321-3497, Tochigi, Japan

    Shinichi Meguro, Noriko Osaki, Noboru Matsuo & Ichiro Tokimitsu

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  1. Shinichi Meguro
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  2. Noriko Osaki
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  3. Noboru Matsuo
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  4. Ichiro Tokimitsu
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Correspondence to Shinichi Meguro.

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Meguro, S., Osaki, N., Matsuo, N. et al. Effect of diacylglycerol on the development of impaired glucose tolerance in sucrose-fed rats. Lipids 41, 347–355 (2006). https://doi.org/10.1007/s11745-006-5105-7

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