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Association of ceramides in human plasma with risk factors of atherosclerosis

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Lipids

Abstract

Atherosclerosis is a multifactorial disorder. Recent studies indicate that the plasma level of sphingomyelin, which yields ceramide, correlates with the risk of coronary heart disease. Therefore, ceramide, a well-known lipid causing apoptosis in various cell types, may contribute to atherogenesis. We examined the relationship between ceramide concentration and risk factors of atherosclerosis in normal human plasma using electrospray tandem mass spectrometry (LC-MS/MS). Major ceramides in human plasma were C24∶0 and C24∶1. The ceramide concentration showed a significant positive correlation with total cholesterol (TC) and triglycerides (TG). In addition, plasma ceramide level increased drastically at a high level of LDL cholesterol (more than 170 mg/dL). Our previous studies demonstrated that the sum of fragmented and conjugated apolipoprotein B-100 proteins (B-ox), which were products of a radical reaction of LDL as well as plasma, was a reliable index of atherosclerosis. B-ox showed a significant positive correlation with the plasma ceramide level. Based on these results, we propose that the ceramide level in human plasma is a risk factor at the early stages of atherosclerosis.

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Abbreviations

apoB:

apolipoprotein B-100

MT:

intima-media thickness of the carotid artery

oxLDL:

oxidized LDL

SM:

sphingomyelin

SMase:

sphingomyelinase

SPT:

serine palmitoyl-CoA transferase

TC:

total cholesterol

TG:

triglyceride

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Nara Women's University, 630-8506, Nara, Japan

    Ikuyo Ichi, Kayoko Nakahara, Yavoi Miyashita, Atsuko Hidaka, Sahoko Kutsukake, Kana Inoue & Shosuke Kojo

  2. Social Insurance Saitama Chuo Hospital, 336-0002, Kitaurawa, Saitama, Japan

    Taro Maruyama

  3. Department of Clinical Pharmacology, Faculty of Medical Sciences, Kyushu University, 812-8582, Fukuoka, Japan

    Yoshikazu Miwa

  4. Department of Bioscience, National Cardiovascular Center Research Institute, Suita, 565-8565, Osaka, Japan

    Mariko Harada-Shiba

  5. Department of Geriatric Medicine of Atami Hospital, International University of Health and Welfare, Atami, 413-0012, Shizuoka, Japan

    Motoo Tsushima

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  1. Ikuyo Ichi
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Correspondence to Shosuke Kojo.

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Ichi, I., Nakahara, K., Miyashita, Y. et al. Association of ceramides in human plasma with risk factors of atherosclerosis. Lipids 41, 859–863 (2006). https://doi.org/10.1007/s11745-006-5041-6

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