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Alterations of high density lipoprotein subclasses in obese subjects

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Lipids

Abstract

The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI). Apoprotein A-I (apoA-I) contents of plasma HDL subclasses were determined by 2-D gel electrophoresis associated with an immunodetection method. The concentrations of TG and the apoA-I content of pre-α1-HDL were significantly higher (P<0.01 and P<0.01, respectively), but the levels of HDL cholesterol, and the apoA-I contents of HDL2a and HDL2b were significantly lower (P<0.01, P<0.05, and P<0.01, respectively) in obese subjects than in subjects having a desirable weight. Moreover, with the elevation of BMI, small-sized pre-α1-HDL increased gradually and significantly, whereas large-sized HDL2b decreased gradually and significantly. Meanwhile, the variations in HDL subclass distribution were more obvious with the elevation of TG levels in obese as well as overweight subjects. In addition, Pearson correlation analysis revealed that BMI and TG levels were positively correlated with pre-α1-HDL but negatively correlated with HDL2b. Multiple regression analysis also showed that TG concentrations were associated independently and positively with high pre-α1-HDL and independently and negatively with low HDL2b in obese and overweight subjects. The HDL particle size was smaller in obese and overweight subjects. The shift to smaller size was more obvious with the elevation of BMI and TG, especially TG levels. These observations, in turn, indicated that HDL maturation might be abnormal, and reverse cholesterol transport might be impaired.

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Abbreviations

ApoA-I:

apoprotein A-I

BMI:

body mass index

CE:

cholesteryl ester

CHD:

coronary heart disease

CM:

chylomicrons

HDL-C:

HDL cholesterol

HL:

hepatic lipase

LDL-C:

LDL cholesterol

LPL:

lipoprotein lipase

PLTP:

phospholipids transfer protein

RCT:

reverse cholesterol transport

SRC:

standardized regression coefficient

TC:

total cholesterol

References

  1. Gordon, T., Castelli, W.P., Hjortland, M.C., Kannel, W.B., and Dawber, T.R. (1977) High Density Lipoprotein as a Protective Factor Against Coronary Heart Disease. The Framingham Study, Am. J. Med., 62, 707–714.

    Article  PubMed  CAS  Google Scholar 

  2. Gordon, D.J., Probstfield, J.L., Garrison, R.J., Neaton, J.D., Castelli, W.P., Knoke, J.D., Jacobs, D.R. Jr., Bangdiwala, S., and Tyroler, H.A., (1989) High-Density Lipoprotein Cholesterol and Cardiovascular Disease. Four Prospective American Studies, Circulation, 79, 8–15.

    PubMed  CAS  Google Scholar 

  3. Wilson, P.W.F., D'Agostino, R.B., Levy, D., Belanger, A.M., Silbershatz, H., and Kannel, W.B. (1998) Prediction of Coronary Heart Disease Using Risk Factor Categories, Circulation 97, 1837–1847.

    PubMed  CAS  Google Scholar 

  4. Goldbourt, U., Yaari, S., and Medalie, J.H. (1997) Isolated Low HDL Cholesterol as a Risk Factor for Coronary Heart Disease Mortality: A 21-Year Follow-up of 8000 Men, Arterioscler. Thromb. Vasc. Biol. 17, 107–113.

    PubMed  CAS  Google Scholar 

  5. Fielding, C.J., and Fielding, P.E. (1995) Molecular Physiology of Reverse Cholesterol Transport, J. Lipid Res., 36, 211–228.

    PubMed  CAS  Google Scholar 

  6. Cheung, M.C., Brown, B.G., and Wolf, A.C. (1991) Altered Particle Size Distribution of apoAI-Containing HDL Subpopulations in Patients with Coronary Heart Disease, Arterioscler. Thromb. Vasc. Biol., 32, 383–394.

    CAS  Google Scholar 

  7. Atger, V., Giral, P., Simon, A., Cambillau, M., Levenson, J., Gariepy, J., Megnien, J.L., and Moatti, N. (1995) High-Density Lipoprotein Subfractions as Markers of Early Atherosclerosis, Am. J. Cardiol., 75, 127–131.

    Article  PubMed  CAS  Google Scholar 

  8. Von Eckardstein, A., Huang, Y., and Assmann, G. (1994) Physiological Role and Clinical Relevance of High-Density Lipoprotein Subclasses, Curr. Opin. Lipidol., 5, 404–416.

    Article  Google Scholar 

  9. Patsch, W., Schonfeld, G., Gotto, A.M., Jr., and Patsch, J.R. (1980) Characterization of Human High Density Lipoproteins by Zonal Ultracentrifugation, J. Biol. Chem., 255, 3178–3185.

    PubMed  CAS  Google Scholar 

  10. Chung, B.H., Wilkinson, T., Geer, J.C., and Segrest, J.P. (1980) Preparative and Quantitative Isolation Of Plasma Lipoproteins: Rapid Single Discontinuous Density Gradient Ultracentrifugation in a Vertical Rotor, J. Lipid Res., 21, 284–291.

    PubMed  CAS  Google Scholar 

  11. Albers, J.J., Warnick, G.R., Wiebe, D., King, P., Steiner, P., Smith, L., Breckenridge, C., Chow, A., Kuba, K., Weidman, S., et al. (1978) Multi-Laboratory Comparison of Three Heparin-Mn2+ Precipitation Procedures for Estimating Cholesterol in High Density Lipoprotein, Clin. Chem., 24, 853–856.

    PubMed  CAS  Google Scholar 

  12. Soedamah-Muthu, S.S., Colhoun, H.M., Thomason, M.J., Betteridge, D.J., Durrington, P.N., Hitman, G.A., Fuller, J.H., Julier, K., Mackness, M.I., Neil, H.A., and CARDS Investigators (2003) The Effect of Atorvastatin on Serum Lipids, Lipoproteins and NMR Spectroscopy Defined Lipoprotein Subclasses in Type 2 Diabetic Patients with Ischaemic Heart Disease, Atherosclerosis, 167, 243–255.

    Article  PubMed  CAS  Google Scholar 

  13. Williams, P.T., Krauss, R.M., Nichols, A.V., Vranizan, K.M., and Wood, P.D. (1990) Identifying the Predominant Peak Diameter of High-Density and Low-Density Lipoproteins by Electrophoresis, J. Lipid Res., 31, 1131–1139.

    PubMed  CAS  Google Scholar 

  14. Wu, X.W., Fu, M.D., and Liu, B.W. (1999) Study on the Immunodetection Method of HDL Subclasses in Human Serum, Chin. J. Arterioscler. 7, 253–255.

    CAS  Google Scholar 

  15. Xu, Y.H., and Fu, M.D. (2003) Alterations of HDL Subclasses in Hyperlipidemia, Clin. Chim. Acta, 332, 95–102.

    Article  PubMed  CAS  Google Scholar 

  16. Johansson, J., Carlson, L.A., Landou, C., and Hamsten, A. (1991) High Density Lipoproteins and Coronary Atherosclerosis. A Strong Inverse Relation with the Largest Particles Confined to Normotriglyceridemic Patients, Arterioscler. Thromb. 11, 174–182.

    PubMed  CAS  Google Scholar 

  17. Barbaras, R., Puchois, P., Fruchart, J.C., and Ailhaud, G. (1987) Cholesterol Efflux from Cultured Adipose Cells Is Mediated by LpAI Particles but Not by LpAI: AII Particles, Biochem. Biophys. Res. Commun., 142, 63–69.

    Article  PubMed  CAS  Google Scholar 

  18. Hubert, H.B., Feinleib, M., McNamara, P.M., and Castelli, W.P. (1983) Obesity as an Independent Risk Factor for Cardiovascular Disease: A 26-Year Follow-up on Participants in the Framingham Heart Study, Circulation, 67, 968–977.

    PubMed  CAS  Google Scholar 

  19. Bertiere, M.C., Fumerun, F., Rigaud, D., Malon, D., Apfelbaum, M., and Girard-Globa, A. (1998) Low High Density Lipoprotein-2 Concentrations in Obese Male Subjects, Atherosclerosis 73, 57–61.

    Article  Google Scholar 

  20. Despres, J.P., Lemieux, I., Dagenais, G.R., Cantin, B., and Lamarche, B. (2000) HDL-Cholesterol as a Marker of Coronary Heart Disease Risk: The Quebec Cardiovascular Study, Atherosclerosis 153, 263–272.

    Article  PubMed  CAS  Google Scholar 

  21. Assmann, G., and Schulte, H. (1992) Relation of High-Density Cholesterol and Triglycerides to Incidence of Atherosclerotic Coronary Artery Disease (the PROCAM Experience), Am. J. Cardiol., 70, 733–737.

    Article  PubMed  CAS  Google Scholar 

  22. Hokanson, J.E., and Austin, M.A. (1996) Plasma Triglyceride Is a Risk Factor for Cardiovascular Disease Independent of High-Density Lipoprotein Cholesterol: A Meta-analysis of Population-Based Prospective Studies, J. Cardiovasc. Risk 3, 213–219.

    Article  PubMed  CAS  Google Scholar 

  23. Cooperative Meta-analysis Group of China Task Force (2002) Predictive Values of Body Mass Index and Waist Circumference to Risk Factors of Related Diseases in Chinese Adult Population, Chin. J. Epidemiol. 23, 5–10.

    Google Scholar 

  24. Executive Summary of the Third Report of the National Cholesterol Education Program (2001) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III), JAMA 285, 486–497.

    Google Scholar 

  25. Kissebah, A.H., and Peiris, A.N. (1989) Biology of Regional Body Fat Distribution: Relationship to NIDDM, Diabetes Metab. Rev. 5, 83–109.

    Article  PubMed  CAS  Google Scholar 

  26. Kissebah, A.H., Freedman, D.S., and Peiris, A.N. (1989) Health Risks of Obesity, Med. Clin. N. Am. 73, 111–138.

    PubMed  CAS  Google Scholar 

  27. Despres, J.P., Moorjani, S., Lupien, P.J., Tremblay, A., Nadeau, A., and Bouchard, C. (1990) Regional Distribution of Body Fat, Plasma Lipoproteins, and Cardiovascular Disease, Arteriosclerosis 10, 497–511.

    PubMed  CAS  Google Scholar 

  28. Despres, J.P. (1991) Obesity and Lipid Metabolism: Relevance of Body Fat Distribution, Curr. Opin. Lipidol. 2, 5–15.

    Article  CAS  Google Scholar 

  29. Gou, L., Fu, M., Xu, Y., Tian, Y., Yan, B., and Yang, L. (2005) Alterations of High Density Lipoprotein Subclasses in Endogenous Hypertriglyceridemia, Am. Heart J. 150, 1039–1045.

    Article  PubMed  CAS  Google Scholar 

  30. Yang, Y.Y., Yan, B.Y., Fu, M.D., Xu, Y., and Tian, Y. (2005) Relationship Between Plasma Lipid Concentrations and HDL Subclasses, Clin. Chim. Acta 354, 49–58.

    Article  PubMed  CAS  Google Scholar 

  31. Jia, L.Q., Bai, H., Fu, M.D., Xu, Y., Yang, Y., Long, S. (2005) Relationship Between Plasma HDL Subclasses Distribution and apoA-I Gene Polymorphisms, Clin. Chim. Acta 360, 37–45.

    Article  PubMed  CAS  Google Scholar 

  32. Long, S.Y., Tian, Y., Zhang, R., Yang, L., Xu, Y., Jia, L., and Fu, M.D. (2006) Relationship Between Plasma HDL Subclasses Distribution and Lipoprotein Lipase Gene Hind III Polymorphism in Hyperlipidemia, Clin. Chim. Acta 366, 316–321.

    Article  PubMed  CAS  Google Scholar 

  33. Rye, K.A., Clay, M.A., and Barter, P.J. (1999) Remodeling of High Density Lipoproteins by Plasma Factors, Atherosclerosis 145, 227–238.

    Article  PubMed  CAS  Google Scholar 

  34. Saidi, Y., Sich, D., Camproux, A., Egloff, M., Federspiel, M.C., Gautier, V., Raisonnier, A., Turpin, G., and Beucler, I. (1999) Interrelationships Between Postprandial Lipoprotein B: CIII Particles Changes and High-Density Lipoprotein Subclass Profiles in Mixed Hyperlipoproteinemia, Metabolism 48, 60–67.

    Article  PubMed  CAS  Google Scholar 

  35. Barrans, A., Collet, X., Barbaras, R., Jaspard, B., Manent, J., Vieu, C., Chap, H., and Perret, B. (1994) Hepatic Lipase Induces the Formation of Pre-β-1-High Density Lipoprotein (HDL) from Triacylglycerol-Rich HDL2, J. Biol. Chem. 269, 11572–11577.

    PubMed  CAS  Google Scholar 

  36. Miida, T., Yamaguchi, T., Tsuda, T., and Okada, M. (1998) High Pre β1-HDL Levels in Hypercholesterolemia Are Maintained by Probucol but Reduced by a Low-Cholesterol Diet, Atherosclerosis 138, 129–134.

    Article  PubMed  CAS  Google Scholar 

  37. Lee, M., Kim, J.Q., Kim, J., Oh, H., and Park, M. (2001) Studies on the Plasma Lipid Profiles, and LCAT and CETP Activities According to Hyperlipoproteinemia Phenotypes (HLP), Atherosclerosis 159, 381–389.

    Article  PubMed  CAS  Google Scholar 

  38. Albers, J.J., Pitman, W., Wolfbauer, G., Cheung, M.C., Kennedy, H., Tu, A.Y., Marcovina, S.M., and Paigen, B. (1999) Relationship Between Phospholipid Transfer Protein Activity and HDL Level and Size Among Inbred Mouse Strains, J. Lipid Res. 40, 295–297.

    PubMed  CAS  Google Scholar 

  39. Grundy, S.M., and Vega, G.L. (1992) Two Different Views of the Relationship of Hypertriglyceridemia to Coronary Heart Disease. Implications for Treatment, Arch. Intern. Med. 152, 28–34.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China

    Li Tian, Mingde Fu, Haoming Tian & Yuye Yang

  2. Department of Biochemistry, Liaoning University of Traditional Chinese Medicine, Shenyang Liaoning, PR China

    Lianqun Jia

  3. Department of Biochemistry and Molecular Biology, Nanhua University, Hengyang, Human, PR China

    Ying Tian

  4. Hoist Group Postdoctoral Work Station, Chengdu, Sichuan, PR China

    Yanhua Xu

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  1. Li Tian
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  2. Lianqun Jia
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  3. Mingde Fu
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  4. Ying Tian
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  5. Yanhua Xu
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  6. Haoming Tian
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  7. Yuye Yang
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Correspondence to Mingde Fu.

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The first two authors contributed equally to this study.

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Tian, L., Jia, L., Fu, M. et al. Alterations of high density lipoprotein subclasses in obese subjects. Lipids 41, 789–796 (2006). https://doi.org/10.1007/s11745-006-5032-7

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  • DOI: https://doi.org/10.1007/s11745-006-5032-7

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