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Impact of 20∶4n−6 supplementation on the fatty acid composition and hemocyte parameters of the pacific oyster crassostrea gigas

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Lipids

Abstract

Arachidonic acid (20∶4n−6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 μg ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by hemocytes from ArA-supplemented oysters. Moreover, the inhibitory effect of Vibrio aestuarianus extracellular products on the adhesive proprieties of hemocytes was lessened in oysters fed ArA-supplemented T-Iso. All changes in oyster hemocyte parameters reported in the present study suggest that ArA and/or eicosanoid metabolites affect oyster hemocyte functions.

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Abbreviations

AASH:

anti-aggregant solution for hemocytes

ArA:

arachidonic acid

AU:

arbitrary units

DCFH-DA:

2′7′-dichlorofluorescein diacetate

DPI:

diphenylene iodonium chloride

DW:

dry weight

ECP:

extracellular products

FSSW:

filtered sterile seawater

ROS:

reactive oxygen species

WW:

wet weight

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Author notes

  1. Maryse Delaporte

    Present address: Department of Pathology and Microbiology, Atlantic Veterinary College, C1A 4P3, Charlottetown, Prince Edward Island, Canada

Authors and Affiliations

  1. UMR 100 Physiologie and Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280, Plouzané, France

    Maryse Delaporte, Jeanne Moal, Emilie Giudicelli, Catherine Séguineau & Jean-François Samain

  2. Laboratoire des Sciences de l'Environnement Marin, UMR 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopôle Brest-Iroise, 29280, Plouzané, France

    Philippe Soudant & Christophe Lambert

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  1. Maryse Delaporte
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Correspondence to Philippe Soudant.

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Delaporte, M., Soudant, P., Moal, J. et al. Impact of 20∶4n−6 supplementation on the fatty acid composition and hemocyte parameters of the pacific oyster crassostrea gigas . Lipids 41, 567–576 (2006). https://doi.org/10.1007/s11745-006-5006-9

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