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Butters Varying in trans 18:1 and cis-9,trans-11 Conjugated Linoleic Acid Modify Plasma Lipoproteins in the Hypercholesterolemic Rabbit

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Lipids

Abstract

The experiment was designed to study the effects of butters differing in conjugated linoleic acid (CLA) and trans 18:1 contents on lipoproteins associated with the risk of atherogenesis. New Zealand White male rabbits (9.6 weeks; 2.1 kg) were assigned for 6 or 12 weeks to three diets (n = 6 per diet) made of conventional pellets with 0.2% cholesterol and with 12% fat provided from a butter poor in trans-10 and trans-11 18:1 and in CLA (standard group), or rich in trans-10 18:1 (trans-10 18:1 group) or rich in trans-11 18:1 and in cis-9,trans-11 CLA (trans-11 18:1/CLA group). Blood samples were collected at the end of dietary treatments. Lipoproteins were separated by gradient-density ultracentrifugation. Lipid classes were determined enzymatically and apolipoproteins A-I and B by radial immunodiffusion. Mainly in the 12-week rabbits, higher plasma triglycerides and apolipoprotein B levels shown in the standard and trans-10 18:1 groups compared with those in the trans-11 18:1/CLA group are associated with higher plasma levels of very low density lipoproteins (VLDL) and low density lipoproteins (LDL) also shown in these two groups. In the 12-week rabbits, a shift towards denser LDL, considered as more atherogenic, was shown only in the trans-10 18:1 group. In these animals, the VLDL + LDL to HDL ratio was 1.7–2.3 times higher in the trans-10 18:1 group than in the other groups (P = 0.076). These results suggest a rather neutral effect of trans-11 18:1/CLA butter towards the risk of atherogenesis, whereas trans-10 18:1 butter would tend to be detrimental.

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Abbreviations

CE:

Cholesteryl esters

CETP:

Cholesteryl ester transfer protein

CHD:

Coronary heart disease

CLA:

Conjugated linoleic acid

FC:

Free cholesterol

HDL:

High density lipoprotein

IDL:

Intermediate density lipoprotein

INRA:

Institut National de la Recherche Agronomique

LDL:

Low density lipoprotein

NEFA:

Nonesterified fatty acids

PHVO:

Partially hydrogenated vegetable oils

PL:

Phospholipids

PUFA:

Polyunsaturated fatty acids

TC:

Total cholesterol

TG:

Triacylglycerols

VA:

Vaccenic acid

VLDL:

Very low density lipoproteins

References

  1. Bauchart D, Durand D, Laplaud PM, Forgez P, Goulinet S, Chapman MJ (1989) Plasma lipoproteins and apolipoproteins in the preruminant calf, bos spp: density distribution, physicochemical properties, and the in vivo evaluation of the contribution of the liver to lipoprotein homeostasis. J Lipid Res 30:1499–1514

    PubMed  CAS  Google Scholar 

  2. Bauman DE, Baumgard LH, Corl BA, Griinari JM (1999) Biosynthesis of conjugated linoleic acid in ruminants. Proc Am Soc Anim Sci 1–15

  3. Bauman DE, Barbano DM, Dwyer DA, Griinari JM (2000) Production of butter with enhanced conjugated linoleic acid for use in biomedical studies with animal model. J Dairy Sci 83:2422–2425

    Article  PubMed  CAS  Google Scholar 

  4. Bauman JM, Griinari JM (2003) Nutritional regulation of milk fat synthesis. Ann Rev Nutr 23:203–227

    Article  CAS  Google Scholar 

  5. Baylin A, Kabagambe EK, Ascherio A, Spiegelman D, Campos H (2003) High 18:2 trans-fatty acids in adipose tissue are associated with increased risk of nonfatal acute myocardial infaction in costa rican adults. J Nutr 133:1186–1191

    PubMed  CAS  Google Scholar 

  6. Chiang MT, Lu YS (1996) Variation of plasma cholesterol levels in rats fed trans fatty acids or cis fatty acids. Int J Vitam Nutr Res 66:263–269

    PubMed  CAS  Google Scholar 

  7. Chilliard Y, Ferlay A, Mansbridge RM, Doreau M (2000) Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. Ann Zootech 49:181–205

    Article  CAS  Google Scholar 

  8. Chilliard Y, Ferlay A (2004) Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reprod Nutr Dev 44:467–492

    Article  PubMed  CAS  Google Scholar 

  9. Enser M, Scollan ND, Choi NJ, Kurt E, Hallet K, Wood JD (1999) Effect of dietary lipid on the content of CLA in beef muscle. Anim Sci 69:143–146

    CAS  Google Scholar 

  10. Fan J, Watanabe T (2000) Cholesterol-fed and transgenic rabbit models for the study of atherosclerosis. J Atheroscler Thromb 7:26–32

    PubMed  CAS  Google Scholar 

  11. Gatto LM, Lyons MA, Brown AJ, Samman S (2002) Trans fatty acids affect lipoprotein metabolism in rats. J Nutr 132:1242–1248

    PubMed  CAS  Google Scholar 

  12. Griinari JM, Bauman DE (1999) Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk fat in ruminants. In: Yurawecz MP, Mossoba MM, Kramer JKG, Pariza MW, Nelson GJ (eds) Advances in conjugated linoleic acid research, vol 1. AOCS Press, Champaign, pp 180–200

  13. Guidetti AM, Beynen AC, Lemmens AG, Gnoni G, Geelen JH (2003) Hepatic fatty acid metabolism in rats fed diets with different contents of C18:0, C18:1 Cis and C18:1 trans isomers. Br J Nutr 90:887–893

    Article  CAS  Google Scholar 

  14. Guyard-Dangremont V, Desrumaux C, Gambert P, Lallemant C, Lagrost L (1998) Phospholipid and cholesteryl ester transfer activities in plasma from 14 vertebrate species. Relation to atherogenesis susceptibility. Comp Biochem Physiol 120:517–525

    Article  CAS  Google Scholar 

  15. Hunter JE (2005) Dietary levels of trans-fatty acids: basis for health concerns and industry efforts to limit use. Nutr Res 25:499–513

    Article  CAS  Google Scholar 

  16. Khosla P, Fungwe T (2001) Conjugated linoleic acid : effects on plasma lipids and cardiovascular function. Curr Opin Lipidol 12:31–34

    Article  PubMed  CAS  Google Scholar 

  17. Khosla P, Sundram K (1996) Effects of dietary fatty acid composition on plasma cholesterol. Prog Lipid Res 35(2):93–132

    Article  PubMed  CAS  Google Scholar 

  18. Kritchevsky D, Tepper SA, Wright S, Tso P, Czarnecki SK (2000) Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J Am Coll Nutr 19:472S–477S

    PubMed  CAS  Google Scholar 

  19. Kritchevsky D, Tepper SA, Wright S, Czarnecki SK (2002) Influenced f graded levels on conjugated linoleicacid (CLA) on experimental athersclerosis in rabbits. Nutr Res 22:1275–1279

    Article  CAS  Google Scholar 

  20. Lee KN, Kritchevski D, Pariza MW (1994) Conjugated linoleic acid and atherosclerosis. Atherosclerosis 108:19–25

    Article  PubMed  CAS  Google Scholar 

  21. Legay F, Bauchart D (1989) Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soya-bean oil. Br J Nutr 61:725–740

    Article  Google Scholar 

  22. Leplaix-Charlat L, Bauchart D, Durand D, Laplaud PM, Chapman MJ (1996) Plasma lipoproteins in preruminant calves fed diets containing tallow or soybean oil with and without cholesterol. J Dairy Sci 79:1267–1277

    PubMed  CAS  Google Scholar 

  23. Lichtenstein A (2000) Trans fatty acids and cardiovascular disease risk. Curr Opin Lipidol 11:37–42

    Article  PubMed  CAS  Google Scholar 

  24. Lock AL, Horne CAM, Baumann D, Salter AM (2005) Butter naturally enriched in conjugated linoleic acid and vaccenic alters tissue fatty acids and improves the plasma lipoprotein profile in cholesterol-fed hamsters. J Nutr 135:1934–1939

    PubMed  CAS  Google Scholar 

  25. Loor JJ, Lin X, Herbein JH (2003) Effects of dietary cis-9,trans-11 18:2, trans-10,cis-12 18:2, or vaccenic acid (trans-11 18:1) during lactation on body composition, tissue fatty acid profiles, and litter growth in mice. Br J Nutr 90:1039–1048

    Article  PubMed  CAS  Google Scholar 

  26. Loor JJ, Ferlay A, Ollier A, Doreau M, Chilliard Y (2005) Relationship among trans and conjugated fatty acids and bovine milk fat yield due to dietary concentrate and linseed oil. J Dairy Sci 88:726–740

    PubMed  CAS  Google Scholar 

  27. Mauger J-F, Lichtenstein A, Ausman L, Jalbert SM, Jauhiainen M, Ehnholm C, Lamarche B (2003) Effect of different forms of dietary hydrogenated fats on LDL particle size. Am J Clin Nutr 78:370–375

    PubMed  CAS  Google Scholar 

  28. Mir PS, Okine EK, Goonewardene L, He ML, Mir Z (2003) Effects of synthetic conjugated linoleic acid (CLA) or bio-formed CLA as high CLA beef on rat growth and adipose tissue development. Can J Anim Sci 83:583–592

    CAS  Google Scholar 

  29. Moseley EE, Powell GL, Riley MB, Jenkins TC (2002) Microbial biohydrogenation of oleic acid to trans isomers in vitro. J Lipid Res 43:290–296

    Google Scholar 

  30. Ness AR, Davey Smith G, Hart C (2001) Milk, coronary heart disease and mortality. J Epidemiol Community Health 55:379–382

    Article  PubMed  CAS  Google Scholar 

  31. Nicolosi RJ, Laitinen L (1996) Dietary conjugated linoleic acid reduces aortic fatty streak formation greater than linoleic acid in hypercholesterolemic hamsters. FASEB J Abst 2751

  32. Oomen CM, Ocke MC, Feskens EJ, Van Erp-Baart MA, Kok FJ, Kromout D (2001) Association between trans fatty acids intake and 10-year risk coronary heart disease in the Zutphen elderly study; a prospective population-based study. Lancet 357:746–751

    Article  PubMed  CAS  Google Scholar 

  33. Ritzenthaler KL, McGuire MK, Falen R, Shultz TD, Dasgupta N, McGuire MA (2001) Estimation of conjugated linoleic acid intake by written dietary assessment methodologies underestimates actual intake evaluated by food duplicate methodology. J Nutr 131:1548–1554

    PubMed  CAS  Google Scholar 

  34. Roy A, Ferlay A, Shingfield KJ, Chilliard Y (2006) Examination of the persistency of milk fatty acid composition responses to plant oils in cows given different basal diets, with particular empahsis on trans-C18:1 fatty acids and isomers of conjugated linoleic acid. Anim Sci 82:479–492

    Article  CAS  Google Scholar 

  35. Roy A, Ferlay A, Chilliard Y (2006) Production of butter rich in trans-10 C18:1 for use in biomedical studies in rodents. Reprod Nutr Dev 46:211–218

    Article  PubMed  CAS  Google Scholar 

  36. SAS/STAT (1989) Guide for personal computers. SAS Institute, Cary

  37. Scislowski V, Bauchart D, Gruffat D, Laplaud PM, Durand D (2005) Effects of dietary n-6 or n-3 PUFA protected or not against ruminal hydrogenations on plasma lipids and their susceptibility to peroxidation in fattening steers. J Anim Sci 83:2162–2174

    PubMed  CAS  Google Scholar 

  38. Sessions VA, Salter AM (1994) The effects of different dietary fats and cholesterol on serum -lipoprotein concentrations in hamsters. Biochim Biophys Acta 1211:207–214

    PubMed  CAS  Google Scholar 

  39. Szymczyk B, Pisulewski P, Szczurek W, Hanczakowski P (2000) The effects of feeding conjugated linoleic (CLA) on rat growth performance, serum lipoproteins and subsequent lipid composition of selected rat tissues. J Sci Food Agric 80:1553–1558

    Article  CAS  Google Scholar 

  40. Valeille K, Férézou J, Amsler G, Quignard-Boulangé A, Parquet M, Gripois D, Dorosvska-Taran V, Martin JC (2005) A Cis-9, trans 11-conjugated linoleic acid-rich oil reduces the outcome of atherogenic process in hyperlipidemic hamster. Am J Physiol Heart Circ Physiol 289:H652–H659

    Article  PubMed  CAS  Google Scholar 

  41. Warensjö E, Jansson J-H, Berglund L, Boman K, Ahrén B, Weinehall L, Lindhal B, Hallmans G, Vessby B (2004) Estimated intake of milk fat is negatively associated with cardiovascular risk factors and does not increase the risk of a first acute myocardial infarction. A prospective case-control study. Br J Nutr 91:635–642

    Article  PubMed  CAS  Google Scholar 

  42. Woollet LA, Daumerie CM, Dietschy JM (1994) Trans-9 octadecenoic acid is biologically neutral and does not regulate the low-density-lipoprotein receptor as the cis isomer does in the hamster. J Lipid Res 35:1661–1673

    Google Scholar 

Download references

Acknowledgements

The authors wish to gratefully acknowledge Marinett Brunel and Anne-Sophie Bage for skillful technical assistance and Daniel Thomas and Philippe Gaydier for the excellent maintenance and care of the animals. The study was supported in part by the AQS CLA Program with financial aid from the French Research and Technology Ministery.

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Authors and Affiliations

  1. UR1213 Herbivores, Research Unit on Herbivores, Nutrients and Metabolisms Group, INRA, Site de Theix, 63122, Saint-Genès-Champanelle, France

    Dominique Bauchart, Stephanie Lorenz, Dominique Gruffat & Denys Durand

  2. INRA, UMR1019, Clermont-Ferrand, 63001, France

    Alexandre Roy, Jean-Michel Chardigny & Jean-Louis Sébédio

  3. CRNH Auvergne, Clermont-Ferrand, 63001, France

    Alexandre Roy, Jean-Michel Chardigny & Jean-Louis Sébédio

  4. UR1213 Herbivores, Research Unit on Herbivores, Adipose Tissue and Milk Lipid Group, INRA, Site de Theix, 63122, Saint-Genès-Champanelle, France

    Alexandre Roy, Anne Ferlay & Yves Chilliard

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  1. Dominique Bauchart
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  2. Alexandre Roy
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  3. Stephanie Lorenz
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  4. Jean-Michel Chardigny
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  5. Anne Ferlay
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Correspondence to Dominique Bauchart.

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Bauchart, D., Roy, A., Lorenz, S. et al. Butters Varying in trans 18:1 and cis-9,trans-11 Conjugated Linoleic Acid Modify Plasma Lipoproteins in the Hypercholesterolemic Rabbit. Lipids 42, 123–133 (2007). https://doi.org/10.1007/s11745-006-3018-0

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